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• W2952433391 endingPage "111331" @default.
• W2952433391 startingPage "111331" @default.
• W2952433391 abstract "This study describes the construction of highly-sensitive photo-electrochemical (PEC) immunosensor for the detection of neuron-specific enolase (NSE). The biosensing platform is comprised of photo-active NiWO4 nanostructures, in-situ-grown over a conductive substrate (indium tin oxide) using a low-temperature template-based co-precipitation approach. The discussed approach enables the formation of discrete, yet morphologically-analogous, nanostructures with complete coverage (pinhole-free) of the electrode surface. The in-situ-grown nanostructure possess dense population with sharp saw-blade like morphological features that can support substantial immobilisation of anti-NSE agent. The constructed platform demonstrated excellent photo-catalytic activity towards uric acid (UA) which served as the base for the Electrochemical -mechanism (EC) based PEC inhibition sensing. The detection of NSE, relied on its obstruction in analytical signal observed for the photo-oxidation of UA after binding to the electrode surface via protein-antibody interaction. The constructed PEC immunosensor exhibits signal sensitivity up to 0.12 ng mL-1 of NSE with excellent signal reproducibility and electrode replicability. Moreover, the constructed platform was successfully used for NSE determination in human serum samples." @default.
• W2952433391 created "2019-06-27" @default.
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• W2952433391 date "2019-09-01" @default.
• W2952433391 modified "2023-09-24" @default.
• W2952433391 title "In-situ growth of NiWO4 saw-blade-like nanostructures and their application in photo-electrochemical (PEC) immunosensor system designed for the detection of neuron-specific enolase" @default.
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• W2952433391 doi "https://doi.org/10.1016/j.bios.2019.111331" @default.
• W2952433391 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31233985" @default.
• W2952433391 hasPublicationYear "2019" @default.
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