Matches in SemOpenAlex for { <https://semopenalex.org/work/W2952603668> ?p ?o ?g. }
- W2952603668 abstract "The chloroplast twin arginine transport (cpTat) system distinguishes itself as a protein transport pathway by translocating fully folded proteins, using the proton-motive force (PMF) as the sole source of energy. The cpTat pathway is evolutionarily conserved with the Tat pathway found in the plasma membrane of many prokaryotes. The cpTat (Escherichia coli) system uses three proteins, Tha4 (TatA), Hcf106 (TatB), and cpTatC (TatC), to form a transient translocase allowing the passage of precursor proteins. Briefly, cpTatC and Hcf106, with Tha4, form the initial receptor complex responsible for precursor protein recognition and binding in an energy-independent manner, while a separate pool of Tha4 assembles with the precursor-bound receptor complex in the presence the PMF. Analysis by blue-native polyacrylamide gel electrophoresis (BN-PAGE) shows that the receptor complex, in the absence of precursor, migrates near 700 kDa and contains cpTatC and Hcf106 with little Tha4 remaining after detergent solubilization. To investigate the role that Hcf106 may play in receptor complex oligomerization and/or stability, systematic cysteine substitutions were made in positions from the N-terminal transmembrane domain to the end of the predicted amphipathic helix of the protein. BN-PAGE analysis allowed us to identify the locations of amino acids in Hcf106 that were critical for interacting with cpTatC. Oxidative cross-linking allowed us to map interactions of the transmembrane domain and amphipathic helix region of Hcf106. In addition, we showed that in vitro expressed, integrated Hcf106 can interact with the precursor signal peptide domain and imported cpTatC, strongly suggesting that a subpopulation of the integrated Hcf106 is participating in competent cpTat complexes." @default.
- W2952603668 created "2019-06-27" @default.
- W2952603668 creator A5004017266 @default.
- W2952603668 creator A5006811862 @default.
- W2952603668 creator A5072148806 @default.
- W2952603668 creator A5086991435 @default.
- W2952603668 date "2018-10-01" @default.
- W2952603668 modified "2023-10-16" @default.
- W2952603668 title "Thylakoid-integrated recombinant Hcf106 participates in the chloroplast twin arginine transport system" @default.
- W2952603668 cites W1485082960 @default.
- W2952603668 cites W1526372251 @default.
- W2952603668 cites W1544155990 @default.
- W2952603668 cites W1967585754 @default.
- W2952603668 cites W1970799971 @default.
- W2952603668 cites W1974160527 @default.
- W2952603668 cites W1975615339 @default.
- W2952603668 cites W1982484796 @default.
- W2952603668 cites W1985205346 @default.
- W2952603668 cites W1991795057 @default.
- W2952603668 cites W1996132538 @default.
- W2952603668 cites W1999767289 @default.
- W2952603668 cites W2000758354 @default.
- W2952603668 cites W2008293899 @default.
- W2952603668 cites W2011371548 @default.
- W2952603668 cites W2031442031 @default.
- W2952603668 cites W2036524595 @default.
- W2952603668 cites W2047624429 @default.
- W2952603668 cites W2051080407 @default.
- W2952603668 cites W2057796783 @default.
- W2952603668 cites W2061775535 @default.
- W2952603668 cites W2070594018 @default.
- W2952603668 cites W2076876639 @default.
- W2952603668 cites W2083665920 @default.
- W2952603668 cites W2085614442 @default.
- W2952603668 cites W2099540110 @default.
- W2952603668 cites W2104543109 @default.
- W2952603668 cites W2109036246 @default.
- W2952603668 cites W2110510188 @default.
- W2952603668 cites W2111165479 @default.
- W2952603668 cites W2117847784 @default.
- W2952603668 cites W2122598117 @default.
- W2952603668 cites W2133245867 @default.
- W2952603668 cites W2140156945 @default.
- W2952603668 cites W2141560226 @default.
- W2952603668 cites W2146236816 @default.
- W2952603668 cites W2147553807 @default.
- W2952603668 cites W2158054139 @default.
- W2952603668 cites W2159071226 @default.
- W2952603668 cites W2163603099 @default.
- W2952603668 cites W2312552384 @default.
- W2952603668 cites W2797201988 @default.
- W2952603668 cites W2952603668 @default.
- W2952603668 cites W4210651271 @default.
- W2952603668 cites W890946837 @default.
- W2952603668 doi "https://doi.org/10.1002/pld3.90" @default.
- W2952603668 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6508782" @default.
- W2952603668 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31245690" @default.
- W2952603668 hasPublicationYear "2018" @default.
- W2952603668 type Work @default.
- W2952603668 sameAs 2952603668 @default.
- W2952603668 citedByCount "6" @default.
- W2952603668 countsByYear W29526036682019 @default.
- W2952603668 countsByYear W29526036682020 @default.
- W2952603668 countsByYear W29526036682021 @default.
- W2952603668 crossrefType "journal-article" @default.
- W2952603668 hasAuthorship W2952603668A5004017266 @default.
- W2952603668 hasAuthorship W2952603668A5006811862 @default.
- W2952603668 hasAuthorship W2952603668A5072148806 @default.
- W2952603668 hasAuthorship W2952603668A5086991435 @default.
- W2952603668 hasBestOaLocation W29526036681 @default.
- W2952603668 hasConcept C101692577 @default.
- W2952603668 hasConcept C104317684 @default.
- W2952603668 hasConcept C10858879 @default.
- W2952603668 hasConcept C113035283 @default.
- W2952603668 hasConcept C118892022 @default.
- W2952603668 hasConcept C12554922 @default.
- W2952603668 hasConcept C138626823 @default.
- W2952603668 hasConcept C167625842 @default.
- W2952603668 hasConcept C170493617 @default.
- W2952603668 hasConcept C181199279 @default.
- W2952603668 hasConcept C24530287 @default.
- W2952603668 hasConcept C2779201268 @default.
- W2952603668 hasConcept C33292294 @default.
- W2952603668 hasConcept C515207424 @default.
- W2952603668 hasConcept C55493867 @default.
- W2952603668 hasConcept C57581600 @default.
- W2952603668 hasConcept C69305403 @default.
- W2952603668 hasConcept C86803240 @default.
- W2952603668 hasConcept C95444343 @default.
- W2952603668 hasConceptScore W2952603668C101692577 @default.
- W2952603668 hasConceptScore W2952603668C104317684 @default.
- W2952603668 hasConceptScore W2952603668C10858879 @default.
- W2952603668 hasConceptScore W2952603668C113035283 @default.
- W2952603668 hasConceptScore W2952603668C118892022 @default.
- W2952603668 hasConceptScore W2952603668C12554922 @default.
- W2952603668 hasConceptScore W2952603668C138626823 @default.
- W2952603668 hasConceptScore W2952603668C167625842 @default.
- W2952603668 hasConceptScore W2952603668C170493617 @default.
- W2952603668 hasConceptScore W2952603668C181199279 @default.
- W2952603668 hasConceptScore W2952603668C24530287 @default.