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- W2952631981 abstract "Chromosome segregation depends on the kinetochore, the machine that establishes force-bearing attachments between DNA and spindle microtubules. Kinetochores are formed every cell cycle via a highly regulated process that requires coordinated assembly of multiple subcomplexes on specialized chromatin. To elucidate the underlying mechanisms, we developed an assay to assemble kinetochores de novo using centromeric DNA and budding yeast extracts. Assembly is enhanced by mitotic phosphorylation of the Dsn1 kinetochore protein and generates kinetochores capable of binding microtubules. We used this assay to investigate why kinetochores recruit the microtubule-binding Ndc80 complex via two receptors: the Mis12 complex and CENP-T. Although the CENP-T pathway is non-essential in yeast, we demonstrate that it becomes essential for viability and Ndc80c recruitment when the Mis12 pathway is crippled by defects in Dsn1 phosphorylation. Assembling kinetochores de novo in yeast extracts provides a powerful and genetically tractable method to elucidate critical regulatory events in the future." @default.
- W2952631981 created "2019-06-27" @default.
- W2952631981 creator A5005300159 @default.
- W2952631981 creator A5014753981 @default.
- W2952631981 creator A5074616878 @default.
- W2952631981 date "2018-08-17" @default.
- W2952631981 modified "2023-10-02" @default.
- W2952631981 title "An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast" @default.
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- W2952631981 doi "https://doi.org/10.7554/elife.37819" @default.
- W2952631981 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6097842" @default.
- W2952631981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30117803" @default.
- W2952631981 hasPublicationYear "2018" @default.
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