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- W2952980588 abstract "Materials capable of directing cell fate by providing spatially-graded mechanical and biomolecular cues are critically important in the reconstitution of living matter. Herein, we report a multi-component inkjet bioprinting method that allows for spatially varying composition and network properties in cell-instructive glycosaminoglycan (GAG)-based biohybrid and pure poly(ethylene glycol) hydrogels with unprecedented (50 μm) resolution. The principle relies on the covalent crosslinking of different polymeric precursors through a very rapid bio-orthogonal Michael type addition scheme adjusted in ways to occur during the fusion of bio-ink droplets prior to and upon contact with the target. Exemplary data show that chemotactic molecular gradients produced by this approach within printed GAG-gels of defined zonal architecture can effectively direct migratory activity and morphogenesis of embedded human bone-marrow derived mesenchymal stem cells. The introduced methodology is expected to enable a new, holistic level of control over reductionistic tissue and organoid models." @default.
- W2952980588 created "2019-06-27" @default.
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- W2952980588 date "2019-07-11" @default.
- W2952980588 modified "2023-10-17" @default.
- W2952980588 title "High resolution bioprinting of multi-component hydrogels" @default.
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- W2952980588 doi "https://doi.org/10.1088/1758-5090/ab2aa1" @default.
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