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- W29529963 abstract "A cotransformation system using somatic embryos was developed to produce grapevines free of selectable marker genes. This was achieved by transforming Vitis vinifera L. “Thompson Seedless” somatic embryos with a mixture of two Agrobacterium strains. The first strain contained a binary plasmid with an egfp gene of interest between the T-DNA borders. The second strain harbored the neomycin phosphotransferase (nptII) gene for positive selection and the cytosine deaminase (codA) gene for negative selection, linked together by a bidirectional dual promoter complex. Our technique included a short positive selection phase of cotransformed somatic embryos on liquid medium containing 100 mg/L kanamycin before subjecting cultures to prolonged negative selection on medium containing 250 mg/L 5-fluorocytosine." @default.
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- W29529963 date "2012-01-01" @default.
- W29529963 modified "2023-09-27" @default.
- W29529963 title "Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes" @default.
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- W29529963 doi "https://doi.org/10.1007/978-1-61779-558-9_17" @default.
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