Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953042703> ?p ?o ?g. }
- W2953042703 endingPage "1748" @default.
- W2953042703 startingPage "1735" @default.
- W2953042703 abstract "Iron deficiency is a serious agricultural problem, particularly in alkaline soils. Secretion of coumarins by Arabidopsis thaliana roots is induced under iron deficiency. An essential enzyme for the biosynthesis of the major Arabidopsis coumarins, scopoletin and its derivatives, is Feruloyl-CoA 6′-Hydroxylase1 (F6′H1), which belongs to a large enzyme family of the 2-oxoglutarate and Fe2+-dependent dioxygenases. We have functionally characterized another enzyme of this family, which is a close homologue of F6′H1 and is encoded by a strongly iron-responsive gene, At3g12900. We purified At3g12900 protein heterologously expressed in Escherichia coli and demonstrated that it is involved in the conversion of scopoletin into fraxetin, via hydroxylation at the C8 position, and that it thus functions as a scopoletin 8-hydroxylase (S8H). Its function in plant cells was confirmed by the transient expression of S8H protein in Nicotiana benthamiana leaves, followed by metabolite profiling and biochemical and ionomic characterization of Arabidopsis s8h knockout lines grown under various iron regimes. Our results indicate that S8H is involved in coumarin biosynthesis, as part of mechanisms used by plants to assimilate iron." @default.
- W2953042703 created "2019-06-27" @default.
- W2953042703 creator A5001376012 @default.
- W2953042703 creator A5014582871 @default.
- W2953042703 creator A5027956126 @default.
- W2953042703 creator A5033300577 @default.
- W2953042703 creator A5035808256 @default.
- W2953042703 creator A5046424792 @default.
- W2953042703 creator A5071465958 @default.
- W2953042703 creator A5078140856 @default.
- W2953042703 creator A5085005880 @default.
- W2953042703 creator A5087017796 @default.
- W2953042703 date "2018-01-18" @default.
- W2953042703 modified "2023-10-12" @default.
- W2953042703 title "Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis" @default.
- W2953042703 cites W102715436 @default.
- W2953042703 cites W1566827244 @default.
- W2953042703 cites W1588913699 @default.
- W2953042703 cites W1597130528 @default.
- W2953042703 cites W1968824780 @default.
- W2953042703 cites W1975334905 @default.
- W2953042703 cites W1978901208 @default.
- W2953042703 cites W1982251843 @default.
- W2953042703 cites W1998819058 @default.
- W2953042703 cites W2002084790 @default.
- W2953042703 cites W2011764011 @default.
- W2953042703 cites W2021628152 @default.
- W2953042703 cites W2025370856 @default.
- W2953042703 cites W2029327342 @default.
- W2953042703 cites W2031414015 @default.
- W2953042703 cites W2035913075 @default.
- W2953042703 cites W2045397058 @default.
- W2953042703 cites W2046455823 @default.
- W2953042703 cites W2054302409 @default.
- W2953042703 cites W2060273135 @default.
- W2953042703 cites W2065796463 @default.
- W2953042703 cites W2069576582 @default.
- W2953042703 cites W2070831559 @default.
- W2953042703 cites W2073146875 @default.
- W2953042703 cites W2073317899 @default.
- W2953042703 cites W2075034684 @default.
- W2953042703 cites W2086491289 @default.
- W2953042703 cites W2087420618 @default.
- W2953042703 cites W2087582673 @default.
- W2953042703 cites W2087918547 @default.
- W2953042703 cites W2090028236 @default.
- W2953042703 cites W2101987783 @default.
- W2953042703 cites W2106365596 @default.
- W2953042703 cites W2106882534 @default.
- W2953042703 cites W2108733083 @default.
- W2953042703 cites W2113800550 @default.
- W2953042703 cites W2116808860 @default.
- W2953042703 cites W2119750302 @default.
- W2953042703 cites W2124946184 @default.
- W2953042703 cites W2126213436 @default.
- W2953042703 cites W2129055411 @default.
- W2953042703 cites W2129746249 @default.
- W2953042703 cites W2135299792 @default.
- W2953042703 cites W2135983909 @default.
- W2953042703 cites W2141102916 @default.
- W2953042703 cites W2144362235 @default.
- W2953042703 cites W2148751307 @default.
- W2953042703 cites W2150749399 @default.
- W2953042703 cites W2156081954 @default.
- W2953042703 cites W2156145828 @default.
- W2953042703 cites W2156654220 @default.
- W2953042703 cites W2162834837 @default.
- W2953042703 cites W2219795105 @default.
- W2953042703 cites W2244874004 @default.
- W2953042703 cites W2289166828 @default.
- W2953042703 cites W2291877499 @default.
- W2953042703 cites W2546622011 @default.
- W2953042703 cites W2588879639 @default.
- W2953042703 cites W2613901416 @default.
- W2953042703 cites W2626773522 @default.
- W2953042703 cites W4234993691 @default.
- W2953042703 cites W4246757476 @default.
- W2953042703 doi "https://doi.org/10.1093/jxb/ery005" @default.
- W2953042703 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5888981" @default.
- W2953042703 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29361149" @default.
- W2953042703 hasPublicationYear "2018" @default.
- W2953042703 type Work @default.
- W2953042703 sameAs 2953042703 @default.
- W2953042703 citedByCount "76" @default.
- W2953042703 countsByYear W29530427032018 @default.
- W2953042703 countsByYear W29530427032019 @default.
- W2953042703 countsByYear W29530427032020 @default.
- W2953042703 countsByYear W29530427032021 @default.
- W2953042703 countsByYear W29530427032022 @default.
- W2953042703 countsByYear W29530427032023 @default.
- W2953042703 crossrefType "journal-article" @default.
- W2953042703 hasAuthorship W2953042703A5001376012 @default.
- W2953042703 hasAuthorship W2953042703A5014582871 @default.
- W2953042703 hasAuthorship W2953042703A5027956126 @default.
- W2953042703 hasAuthorship W2953042703A5033300577 @default.
- W2953042703 hasAuthorship W2953042703A5035808256 @default.
- W2953042703 hasAuthorship W2953042703A5046424792 @default.
- W2953042703 hasAuthorship W2953042703A5071465958 @default.