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• W2953052523 endingPage "610" @default.
• W2953052523 startingPage "601" @default.
• W2953052523 abstract "RNA splicing, an essential part of eukaryotic pre-messenger RNA processing, can be simultaneous with transcription by RNA polymerase II. Here, we compare and review independent next-generation sequencing methods that jointly quantify transcription and splicing in budding yeast. For many yeast transcripts, splicing is fast, taking place within seconds of intron transcription, while polymerase is within a few dozens of nucleotides of the 3' splice site. Ribosomal protein transcripts are spliced particularly fast and cotranscriptionally. However, some transcripts are spliced inefficiently or mainly post-transcriptionally. Intron-mediated regulation of some genes is likely to be cotranscriptional. We suggest that intermediates of the splicing reaction, missing from current data sets, may hold key information about splicing kinetics." @default.
• W2953052523 created "2019-06-27" @default.
• W2953052523 creator A5042990962 @default.
• W2953052523 creator A5073859869 @default.
• W2953052523 date "2017-02-02" @default.
• W2953052523 modified "2023-09-27" @default.
• W2953052523 title "Extremely fast and incredibly close: cotranscriptional splicing in budding yeast" @default.
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• W2953052523 doi "https://doi.org/10.1261/rna.060830.117" @default.
• W2953052523 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5393171" @default.
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