FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953093977> ?p ?o ?g. }

• W2953093977 abstract "While it has been known for several years that viral RNAs are subject to the addition of several distinct covalent modifications to individual nucleotides, collectively referred to as epitranscriptomic modifications, the effect of these editing events on viral gene expression has been controversial. Here, we report the purification of murine leukemia virus (MLV) genomic RNA to homogeneity and show that this viral RNA contains levels of N6-methyladenosine (m6A), 5-methylcytosine (m5C), and 2'O-methylated (Nm) ribonucleotides that are an order of magnitude higher than detected on bulk cellular mRNAs. Mapping of m6A and m5C residues on MLV transcripts identified multiple discrete editing sites and allowed the construction of MLV variants bearing silent mutations that removed a subset of these sites. Analysis of the replication potential of these mutants revealed a modest but significant attenuation in viral replication in 3T3 cells in culture. Consistent with a positive role for m6A and m5C in viral replication, we also demonstrate that overexpression of the key m6A reader protein YTHDF2 enhances MLV replication, while downregulation of the m5C writer NSUN2 inhibits MLV replication.IMPORTANCE The data presented in the present study demonstrate that MLV RNAs bear an exceptionally high level of the epitranscriptomic modifications m6A, m5C, and Nm, suggesting that these each facilitate some aspect of the viral replication cycle. Consistent with this hypothesis, we demonstrate that mutational removal of a subset of these m6A or m5C modifications from MLV transcripts inhibits MLV replication in cis, and a similar result was also observed upon manipulation of the level of expression of key cellular epitranscriptomic cofactors in trans Together, these results argue that the addition of several different epitranscriptomic modifications to viral transcripts stimulates viral gene expression and suggest that MLV has therefore evolved to maximize the level of these modifications that are added to viral RNAs." @default.
• W2953093977 created "2019-06-27" @default.
• W2953093977 creator A5010232875 @default.
• W2953093977 creator A5012348306 @default.
• W2953093977 creator A5018283700 @default.
• W2953093977 creator A5040596085 @default.
• W2953093977 creator A5045067166 @default.
• W2953093977 creator A5045734619 @default.
• W2953093977 creator A5066041195 @default.
• W2953093977 date "2019-06-25" @default.
• W2953093977 modified "2023-10-13" @default.
• W2953093977 title "Extensive Epitranscriptomic Methylation of A and C Residues on Murine Leukemia Virus Transcripts Enhances Viral Gene Expression" @default.
• W2953093977 cites W1590309899 @default.
• W2953093977 cites W1986988560 @default.
• W2953093977 cites W2018289835 @default.
• W2953093977 cites W2022268135 @default.
• W2953093977 cites W2053003053 @default.
• W2953093977 cites W2060902122 @default.
• W2953093977 cites W2092030692 @default.
• W2953093977 cites W2100837269 @default.
• W2953093977 cites W2106013133 @default.
• W2953093977 cites W2108234281 @default.
• W2953093977 cites W2124985265 @default.
• W2953093977 cites W2126165721 @default.
• W2953093977 cites W2131837157 @default.
• W2953093977 cites W2167917127 @default.
• W2953093977 cites W2176803664 @default.
• W2953093977 cites W2271995006 @default.
• W2953093977 cites W2327216023 @default.
• W2953093977 cites W2336857745 @default.
• W2953093977 cites W2410047653 @default.
• W2953093977 cites W2434565902 @default.
• W2953093977 cites W2462449071 @default.
• W2953093977 cites W2532091902 @default.
• W2953093977 cites W2538543247 @default.
• W2953093977 cites W2555494550 @default.
• W2953093977 cites W2573627990 @default.
• W2953093977 cites W2592106274 @default.
• W2953093977 cites W2606518593 @default.
• W2953093977 cites W2624427473 @default.
• W2953093977 cites W2627064270 @default.
• W2953093977 cites W2754900935 @default.
• W2953093977 cites W2787303145 @default.
• W2953093977 cites W2848484312 @default.
• W2953093977 cites W2898209133 @default.
• W2953093977 cites W2910341377 @default.
• W2953093977 cites W2940752764 @default.
• W2953093977 cites W2953224225 @default.
• W2953093977 cites W2953714283 @default.
• W2953093977 cites W3121719756 @default.
• W2953093977 doi "https://doi.org/10.1128/mbio.01209-19" @default.
• W2953093977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6561033" @default.
• W2953093977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31186331" @default.
• W2953093977 hasPublicationYear "2019" @default.
• W2953093977 type Work @default.
• W2953093977 sameAs 2953093977 @default.
• W2953093977 citedByCount "43" @default.
• W2953093977 countsByYear W29530939772019 @default.
• W2953093977 countsByYear W29530939772020 @default.
• W2953093977 countsByYear W29530939772021 @default.
• W2953093977 countsByYear W29530939772022 @default.
• W2953093977 countsByYear W29530939772023 @default.
• W2953093977 crossrefType "journal-article" @default.
• W2953093977 hasAuthorship W2953093977A5010232875 @default.
• W2953093977 hasAuthorship W2953093977A5012348306 @default.
• W2953093977 hasAuthorship W2953093977A5018283700 @default.
• W2953093977 hasAuthorship W2953093977A5040596085 @default.
• W2953093977 hasAuthorship W2953093977A5045067166 @default.
• W2953093977 hasAuthorship W2953093977A5045734619 @default.
• W2953093977 hasAuthorship W2953093977A5066041195 @default.
• W2953093977 hasBestOaLocation W29530939771 @default.
• W2953093977 hasConcept C104317684 @default.
• W2953093977 hasConcept C140704245 @default.
• W2953093977 hasConcept C150194340 @default.
• W2953093977 hasConcept C153911025 @default.
• W2953093977 hasConcept C159047783 @default.
• W2953093977 hasConcept C2522874641 @default.
• W2953093977 hasConcept C2779562191 @default.
• W2953093977 hasConcept C33288867 @default.
• W2953093977 hasConcept C54355233 @default.
• W2953093977 hasConcept C67705224 @default.
• W2953093977 hasConcept C86803240 @default.
• W2953093977 hasConcept C95444343 @default.
• W2953093977 hasConceptScore W2953093977C104317684 @default.
• W2953093977 hasConceptScore W2953093977C140704245 @default.
• W2953093977 hasConceptScore W2953093977C150194340 @default.
• W2953093977 hasConceptScore W2953093977C153911025 @default.
• W2953093977 hasConceptScore W2953093977C159047783 @default.
• W2953093977 hasConceptScore W2953093977C2522874641 @default.
• W2953093977 hasConceptScore W2953093977C2779562191 @default.
• W2953093977 hasConceptScore W2953093977C33288867 @default.
• W2953093977 hasConceptScore W2953093977C54355233 @default.
• W2953093977 hasConceptScore W2953093977C67705224 @default.
• W2953093977 hasConceptScore W2953093977C86803240 @default.
• W2953093977 hasConceptScore W2953093977C95444343 @default.
• W2953093977 hasIssue "3" @default.
• W2953093977 hasLocation W29530939771 @default.
• W2953093977 hasLocation W29530939772 @default.
• W2953093977 hasLocation W29530939773 @default.
• W2953093977 hasLocation W29530939774 @default.

• next