Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953157439> ?p ?o ?g. }
- W2953157439 abstract "Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas proteins provide an immune-like response in many prokaryotes against extraneous nucleic acids. CRISPR-Cas systems are classified into different classes and types. Class 1 CRISPR-Cas systems form multi-protein effector complexes that includes a guide RNA (crRNA) used to identify the target for destruction. Here we present crystal structures of Staphylococcus epidermidis Type III-A CRISPR subunits Csm2 and Csm3 and a 5.2 Å resolution single-particle cryo-electron microscopy (cryo-EM) reconstruction of an effector subcomplex including the crRNA. The structures help to clarify the quaternary architecture of Type III-A effector complexes, as well as to provide details on crRNA binding, target RNA binding and cleavage, and intermolecular interactions essential for effector complex assembly. The structures allow a better understanding of the organization of Type III-A CRISPR effector complexes as well as highlighting the overall similarities and differences with other Class 1 effector complexes." @default.
- W2953157439 created "2019-06-27" @default.
- W2953157439 creator A5022616937 @default.
- W2953157439 creator A5026477155 @default.
- W2953157439 creator A5034562839 @default.
- W2953157439 date "2018-10-24" @default.
- W2953157439 modified "2023-09-27" @default.
- W2953157439 title "Structural Organization of a Type III-A CRISPR Effector Subcomplex Determined by X-ray Crystallography and Cryo-EM" @default.
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