Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953201991> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2953201991 abstract "Abstract Background Balancer chromosomes are tools used by fruit fly geneticists to prevent meiotic recombination. Recently, CRISPR/Cas9 genome editing has been shown capable of generating inversions similar to the chromosomal rearrangements present in balancer chromosomes. Extending the benefits of balancer chromosomes to other multicellular organisms could significantly accelerate biomedical and plant genetics research. Results Here, we present GRIBCG (Guide RNA Identifier for Balancer Chromosome Generation), a tool for the rational design of balancer chromosomes. GRIBCG identifies single guide RNAs (sgRNAs) for use with Streptococcus pyogenes Cas9 (SpCas9). These sgRNAs would efficiently cut a chromosome multiple times while minimizing off-target cutting in the rest of the genome. We describe the performance of this tool on six model organisms and compare our results to two routinely used fruit fly balancer chromosomes. Conclusion GRIBCG is the first of its kind tool for the design of balancer chromosomes using CRISPR/Cas9. GRIBCG can accelerate genetics research by providing a fast, systematic and simple to use framework to induce chromosomal rearrangements." @default.
- W2953201991 created "2019-06-27" @default.
- W2953201991 creator A5062157355 @default.
- W2953201991 creator A5064810387 @default.
- W2953201991 date "2018-12-02" @default.
- W2953201991 modified "2023-10-16" @default.
- W2953201991 title "GRIBCG: A software for selection of sgRNAs in the design of balancer chromosomes" @default.
- W2953201991 cites W1607870348 @default.
- W2953201991 cites W1879165674 @default.
- W2953201991 cites W1977709885 @default.
- W2953201991 cites W1999903619 @default.
- W2953201991 cites W2036176224 @default.
- W2953201991 cites W2041217592 @default.
- W2953201991 cites W2050480410 @default.
- W2953201991 cites W2158051911 @default.
- W2953201991 cites W2159523911 @default.
- W2953201991 cites W2252568502 @default.
- W2953201991 cites W2379711956 @default.
- W2953201991 cites W2522190706 @default.
- W2953201991 cites W2622033752 @default.
- W2953201991 doi "https://doi.org/10.1101/484360" @default.
- W2953201991 hasPublicationYear "2018" @default.
- W2953201991 type Work @default.
- W2953201991 sameAs 2953201991 @default.
- W2953201991 citedByCount "0" @default.
- W2953201991 crossrefType "posted-content" @default.
- W2953201991 hasAuthorship W2953201991A5062157355 @default.
- W2953201991 hasAuthorship W2953201991A5064810387 @default.
- W2953201991 hasBestOaLocation W29532019911 @default.
- W2953201991 hasConcept C104317684 @default.
- W2953201991 hasConcept C132455925 @default.
- W2953201991 hasConcept C141231307 @default.
- W2953201991 hasConcept C30481170 @default.
- W2953201991 hasConcept C41008148 @default.
- W2953201991 hasConcept C54355233 @default.
- W2953201991 hasConcept C70721500 @default.
- W2953201991 hasConcept C86803240 @default.
- W2953201991 hasConcept C97702854 @default.
- W2953201991 hasConcept C98108389 @default.
- W2953201991 hasConceptScore W2953201991C104317684 @default.
- W2953201991 hasConceptScore W2953201991C132455925 @default.
- W2953201991 hasConceptScore W2953201991C141231307 @default.
- W2953201991 hasConceptScore W2953201991C30481170 @default.
- W2953201991 hasConceptScore W2953201991C41008148 @default.
- W2953201991 hasConceptScore W2953201991C54355233 @default.
- W2953201991 hasConceptScore W2953201991C70721500 @default.
- W2953201991 hasConceptScore W2953201991C86803240 @default.
- W2953201991 hasConceptScore W2953201991C97702854 @default.
- W2953201991 hasConceptScore W2953201991C98108389 @default.
- W2953201991 hasLocation W29532019911 @default.
- W2953201991 hasLocation W29532019912 @default.
- W2953201991 hasLocation W29532019913 @default.
- W2953201991 hasOpenAccess W2953201991 @default.
- W2953201991 hasPrimaryLocation W29532019911 @default.
- W2953201991 hasRelatedWork W2134298288 @default.
- W2953201991 hasRelatedWork W2172149993 @default.
- W2953201991 hasRelatedWork W2344454439 @default.
- W2953201991 hasRelatedWork W2375351600 @default.
- W2953201991 hasRelatedWork W2794852126 @default.
- W2953201991 hasRelatedWork W2898360779 @default.
- W2953201991 hasRelatedWork W2951741674 @default.
- W2953201991 hasRelatedWork W2952884279 @default.
- W2953201991 hasRelatedWork W2994205747 @default.
- W2953201991 hasRelatedWork W3021321114 @default.
- W2953201991 isParatext "false" @default.
- W2953201991 isRetracted "false" @default.
- W2953201991 magId "2953201991" @default.
- W2953201991 workType "article" @default.