FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953202996> ?p ?o ?g. }

• W2953202996 abstract "Ixodes ricinus is the most important vector of tick-borne diseases in Europe. A better knowledge of its genome and transcriptome is important for developing control strategies. Previous transcriptomic studies of I. ricinus have focused on gene expression during the blood meal in specific tissues. To obtain a broader picture of changes in gene expression during the blood meal, our study analysed the transcriptome at the level of the whole body for both nymphal and adult ticks. Ixodes ricinus ticks from a highly inbred colony at the University of Neuchâtel were used. We also analysed previously published RNAseq studies to compare the genetic variation between three wild strains and three laboratory strains, including the strain from Neuchâtel. RNA was extracted from whole tick bodies and the cDNA was sequenced, producing 162,872,698 paired-end reads. Our reference transcriptome contained 179,316 contigs, of which 31% were annotated using Trinotate. Gene expression was compared between ticks that differed by feeding status (unfed vs partially fed). We found that blood-feeding in nymphs and female adult ticks increased the expression of cuticle-associated genes. Using a set of 3866 single nucleotide polymorphisms to calculate the heterozygosity, we found that the wild tick populations of I. ricinus had much higher levels of heterozygosity than the three laboratory populations. Using high throughput strand-oriented sequencing for whole ticks in different stages and feeding conditions, we obtained a de novo assembly that significantly increased the genomic resources available for I. ricinus. Our study illustrates the importance of analysing the transcriptome at the level of the whole body to gain additional insights into how gene expression changes over the life-cycle of an organism. Our comparison of several RNAseq datasets shows the power of transcriptomic data to accurately characterize genetic polymorphism and for comparing different populations or sources of sequencing material." @default.
• W2953202996 created "2019-06-27" @default.
• W2953202996 creator A5014840793 @default.
• W2953202996 creator A5015252334 @default.
• W2953202996 creator A5026690931 @default.
• W2953202996 creator A5045761528 @default.
• W2953202996 creator A5059911817 @default.
• W2953202996 creator A5075063670 @default.
• W2953202996 creator A5076923399 @default.
• W2953202996 creator A5086432078 @default.
• W2953202996 date "2018-06-26" @default.
• W2953202996 modified "2023-09-27" @default.
• W2953202996 title "Whole body transcriptomes and new insights into the biology of the tick Ixodes ricinus" @default.
• W2953202996 cites W1527927437 @default.
• W2953202996 cites W1886649985 @default.
• W2953202996 cites W1944617749 @default.
• W2953202996 cites W1972185194 @default.
• W2953202996 cites W1984180033 @default.
• W2953202996 cites W2006528936 @default.
• W2953202996 cites W2011657487 @default.
• W2953202996 cites W2012540162 @default.
• W2953202996 cites W2014109817 @default.
• W2953202996 cites W2027102545 @default.
• W2953202996 cites W2027828573 @default.
• W2953202996 cites W2045843097 @default.
• W2953202996 cites W2063206666 @default.
• W2953202996 cites W2065131747 @default.
• W2953202996 cites W2065381296 @default.
• W2953202996 cites W2069488864 @default.
• W2953202996 cites W2070874919 @default.
• W2953202996 cites W2075774945 @default.
• W2953202996 cites W2076515648 @default.
• W2953202996 cites W2088863268 @default.
• W2953202996 cites W2089973643 @default.
• W2953202996 cites W2094201044 @default.
• W2953202996 cites W2103635790 @default.
• W2953202996 cites W2128452565 @default.
• W2953202996 cites W2131271579 @default.
• W2953202996 cites W2135297744 @default.
• W2953202996 cites W2136145671 @default.
• W2953202996 cites W2138122982 @default.
• W2953202996 cites W2141584209 @default.
• W2953202996 cites W2142678478 @default.
• W2953202996 cites W2147250916 @default.
• W2953202996 cites W2147526198 @default.
• W2953202996 cites W2152770371 @default.
• W2953202996 cites W2155133651 @default.
• W2953202996 cites W2155628349 @default.
• W2953202996 cites W2156125289 @default.
• W2953202996 cites W2170551349 @default.
• W2953202996 cites W2179438025 @default.
• W2953202996 cites W2235243555 @default.
• W2953202996 cites W2281061049 @default.
• W2953202996 cites W2323326409 @default.
• W2953202996 cites W2395791915 @default.
• W2953202996 cites W2502274642 @default.
• W2953202996 cites W2553951693 @default.
• W2953202996 cites W2586486806 @default.
• W2953202996 cites W2604985075 @default.
• W2953202996 cites W4211143140 @default.
• W2953202996 doi "https://doi.org/10.1186/s13071-018-2932-3" @default.
• W2953202996 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6019515" @default.
• W2953202996 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29941016" @default.
• W2953202996 hasPublicationYear "2018" @default.
• W2953202996 type Work @default.
• W2953202996 sameAs 2953202996 @default.
• W2953202996 citedByCount "22" @default.
• W2953202996 countsByYear W29532029962019 @default.
• W2953202996 countsByYear W29532029962020 @default.
• W2953202996 countsByYear W29532029962021 @default.
• W2953202996 countsByYear W29532029962022 @default.
• W2953202996 countsByYear W29532029962023 @default.
• W2953202996 crossrefType "journal-article" @default.
• W2953202996 hasAuthorship W2953202996A5014840793 @default.
• W2953202996 hasAuthorship W2953202996A5015252334 @default.
• W2953202996 hasAuthorship W2953202996A5026690931 @default.
• W2953202996 hasAuthorship W2953202996A5045761528 @default.
• W2953202996 hasAuthorship W2953202996A5059911817 @default.
• W2953202996 hasAuthorship W2953202996A5075063670 @default.
• W2953202996 hasAuthorship W2953202996A5076923399 @default.
• W2953202996 hasAuthorship W2953202996A5086432078 @default.
• W2953202996 hasBestOaLocation W29532029961 @default.
• W2953202996 hasConcept C104317684 @default.
• W2953202996 hasConcept C143589142 @default.
• W2953202996 hasConcept C150194340 @default.
• W2953202996 hasConcept C162317418 @default.
• W2953202996 hasConcept C180754005 @default.
• W2953202996 hasConcept C18903297 @default.
• W2953202996 hasConcept C190612196 @default.
• W2953202996 hasConcept C2777127260 @default.
• W2953202996 hasConcept C2777923404 @default.
• W2953202996 hasConcept C2778576062 @default.
• W2953202996 hasConcept C2779620486 @default.
• W2953202996 hasConcept C54355233 @default.
• W2953202996 hasConcept C59822182 @default.
• W2953202996 hasConcept C78458016 @default.
• W2953202996 hasConcept C86803240 @default.
• W2953202996 hasConcept C90856448 @default.
• W2953202996 hasConceptScore W2953202996C104317684 @default.
• W2953202996 hasConceptScore W2953202996C143589142 @default.

• next