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• W2953367055 abstract "Although the genome of Trypanosoma cruzi, the causative agent of Chagas disease, was first made available in 2005, with additional strains reported later, the intrinsic genome complexity of this parasite (the abundance of repetitive sequences and genes organized in tandem) has traditionally hindered high-quality genome assembly and annotation. This also limits diverse types of analyses that require high degrees of precision. Long reads generated by third-generation sequencing technologies are particularly suitable to address the challenges associated with T. cruzi’s genome since they permit direct determination of the full sequence of large clusters of repetitive sequences without collapsing them. This, in turn, not only allows accurate estimation of gene copy numbers but also circumvents assembly fragmentation. Here, we present the analysis of the genome sequences of two T. cruzi clones: the hybrid TCC (TcVI) and the non-hybrid Dm28c (TcI), determined by PacBio Single Molecular Real-Time (SMRT) technology. The improved assemblies herein obtained permitted us to accurately estimate gene copy numbers, abundance and distribution of repetitive sequences (including satellites and retroelements). We found that the genome of T. cruzi is composed of a ‘core compartment’ and a ‘disruptive compartment’ which exhibit opposite GC content and gene composition. Novel tandem and dispersed repetitive sequences were identified, including some located inside coding sequences. Additionally, homologous chromosomes were separately assembled, allowing us to retrieve haplotypes as separate contigs instead of a unique mosaic sequence. Finally, manual annotation of surface multigene families, mucins and trans-sialidases allows now a better overview of these complex groups of genes." @default.
• W2953367055 created "2019-06-27" @default.
• W2953367055 creator A5051885185 @default.
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• W2953367055 date "2018-05-01" @default.
• W2953367055 modified "2023-10-18" @default.
• W2953367055 title "Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi" @default.
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• W2953367055 doi "https://doi.org/10.1099/mgen.0.000177" @default.
• W2953367055 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5994713" @default.
• W2953367055 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29708484" @default.
• W2953367055 hasPublicationYear "2018" @default.
• W2953367055 type Work @default.
• W2953367055 sameAs 2953367055 @default.

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