FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953374039> ?p ?o ?g. }

• W2953374039 endingPage "103413" @default.
• W2953374039 startingPage "103413" @default.
• W2953374039 abstract "The overuse of antibiotics to control bacterial pathogens leads to the generation of their antibiotic-resistant strains including Edwardsiella tarda. Understanding of mechanisms of the antibiotic resistance is crucial to develop novel methods to manage the infection. Here, two-dimensional electrophoresis-based proteomics was used to characterize balofloxacin-responsive proteins. The altered proteome consisted of 19 proteins with differential abundance, where six metabolic pathways were enriched. The metabolic modulation activated the central carbon metabolism with elevation of NADH, PMF, and ATP. Among the 19 proteins, ETAE_1987 (pre-peptidase) and ETAE_2174 (integration host factor beta subunit) were bound with balofloxacin directly. This was further confirmed by the binding of balofloxacin with recombinant ETAE_1987 and ETAE_2174 using Oxford cup method. Compared with bovine serum albumin, a known balofloxacin-binding protein, ETAE_1987 and ETAE_2174 increased the binding capability by 3.3- and 22-fold, respectively. The combination was validated by microscale thermophoresis. These data characterize the balofloxacin-stressed proteome as a result of the increased central carbon metabolism and energy metabolism and determine ETAE_1987 and ETAE_2174 as balofloxacin-binding proteins. These findings have significant implications in understanding bacterial antibiotic-resistant and drug action mechanisms based on balofloxacin-binding proteins. SIGNIFICANCE: Antibiotic-resistant Edwardsiella tarda strains are frequently isolated and cause a great loss in aquaculture since these bacterial strains are insensitivity to antibiotics. The present study showed that the increased central carbon metabolism forms a characteristic feature of the balofloxacin-stressed proteomics. Furthermore, two proteins, ETAE_1987 (pre-peptidase) and ETAE_2174, of the balofloxacin-stressed proteome were identified as balofloxacin-binding proteins. The binding capability is 0.39 ± 0.017 and 2.67 ± 0.066 ng/μg proteins for ETAE_1987 and ETAE_2174, respectively. These results reveal the elevated central carbon metabolism as a key feature of the balofloxacin-stressed proteomics and pre-peptidase and integration host factor as balofloxacin-binding proteins in E. tarda. These findings are useful in the understanding of bacterial balofloxacin-stressed mechanisms and providing new targets for controlling antibiotic-resistant bacteria." @default.
• W2953374039 created "2019-06-27" @default.
• W2953374039 creator A5002550765 @default.
• W2953374039 creator A5009934914 @default.
• W2953374039 creator A5026981516 @default.
• W2953374039 creator A5034753099 @default.
• W2953374039 creator A5043019475 @default.
• W2953374039 creator A5063159210 @default.
• W2953374039 creator A5063575773 @default.
• W2953374039 date "2019-08-01" @default.
• W2953374039 modified "2023-10-12" @default.
• W2953374039 title "Characterization of balofloxacin-stressed proteomics and identification of balofloxacin-binding proteins pre-peptidase and integration host factor in Edwardsiella tarda" @default.
• W2953374039 cites W1833253685 @default.
• W2953374039 cites W1884241479 @default.
• W2953374039 cites W1972292552 @default.
• W2953374039 cites W1972980414 @default.
• W2953374039 cites W1973278214 @default.
• W2953374039 cites W1975240449 @default.
• W2953374039 cites W1977316842 @default.
• W2953374039 cites W1986332448 @default.
• W2953374039 cites W1995808283 @default.
• W2953374039 cites W1996665540 @default.
• W2953374039 cites W1996689601 @default.
• W2953374039 cites W1999223533 @default.
• W2953374039 cites W2007296377 @default.
• W2953374039 cites W2009003782 @default.
• W2953374039 cites W2012439771 @default.
• W2953374039 cites W2023149185 @default.
• W2953374039 cites W2024903387 @default.
• W2953374039 cites W2025216709 @default.
• W2953374039 cites W2029871013 @default.
• W2953374039 cites W2034101964 @default.
• W2953374039 cites W2044167045 @default.
• W2953374039 cites W2044190719 @default.
• W2953374039 cites W2046385870 @default.
• W2953374039 cites W2052221476 @default.
• W2953374039 cites W2052514587 @default.
• W2953374039 cites W2052701065 @default.
• W2953374039 cites W2054550126 @default.
• W2953374039 cites W2062094999 @default.
• W2953374039 cites W2062780978 @default.
• W2953374039 cites W2066118409 @default.
• W2953374039 cites W2075423625 @default.
• W2953374039 cites W2088825653 @default.
• W2953374039 cites W2094298224 @default.
• W2953374039 cites W2095846193 @default.
• W2953374039 cites W2097272693 @default.
• W2953374039 cites W2108813080 @default.
• W2953374039 cites W2109276742 @default.
• W2953374039 cites W2113255205 @default.
• W2953374039 cites W2113403474 @default.
• W2953374039 cites W2146103350 @default.
• W2953374039 cites W2155671374 @default.
• W2953374039 cites W2317638455 @default.
• W2953374039 cites W2344798336 @default.
• W2953374039 cites W2464182791 @default.
• W2953374039 cites W2593536290 @default.
• W2953374039 cites W2774244453 @default.
• W2953374039 cites W2790543048 @default.
• W2953374039 cites W2792624183 @default.
• W2953374039 cites W2793325503 @default.
• W2953374039 cites W2799727629 @default.
• W2953374039 cites W2802146985 @default.
• W2953374039 cites W2897688654 @default.
• W2953374039 cites W2897747897 @default.
• W2953374039 cites W2899111084 @default.
• W2953374039 cites W2899560294 @default.
• W2953374039 cites W2902206225 @default.
• W2953374039 cites W2913045108 @default.
• W2953374039 doi "https://doi.org/10.1016/j.jprot.2019.103413" @default.
• W2953374039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31181269" @default.
• W2953374039 hasPublicationYear "2019" @default.
• W2953374039 type Work @default.
• W2953374039 sameAs 2953374039 @default.
• W2953374039 citedByCount "3" @default.
• W2953374039 countsByYear W29533740392019 @default.
• W2953374039 countsByYear W29533740392021 @default.
• W2953374039 countsByYear W29533740392023 @default.
• W2953374039 crossrefType "journal-article" @default.
• W2953374039 hasAuthorship W2953374039A5002550765 @default.
• W2953374039 hasAuthorship W2953374039A5009934914 @default.
• W2953374039 hasAuthorship W2953374039A5026981516 @default.
• W2953374039 hasAuthorship W2953374039A5034753099 @default.
• W2953374039 hasAuthorship W2953374039A5043019475 @default.
• W2953374039 hasAuthorship W2953374039A5063159210 @default.
• W2953374039 hasAuthorship W2953374039A5063575773 @default.
• W2953374039 hasConcept C104317684 @default.
• W2953374039 hasConcept C104397665 @default.
• W2953374039 hasConcept C185592680 @default.
• W2953374039 hasConcept C2776832872 @default.
• W2953374039 hasConcept C46111723 @default.
• W2953374039 hasConcept C55493867 @default.
• W2953374039 hasConcept C86803240 @default.
• W2953374039 hasConcept C89423630 @default.
• W2953374039 hasConceptScore W2953374039C104317684 @default.
• W2953374039 hasConceptScore W2953374039C104397665 @default.
• W2953374039 hasConceptScore W2953374039C185592680 @default.
• W2953374039 hasConceptScore W2953374039C2776832872 @default.

• next