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• W2953452020 abstract "Expansins are cell wall‐loosening proteins found in all land plants and many microbial species. Despite homologous structures, bacterial expansins have much weaker cellulose binding and wall‐loosening activity than plant expansins. We hypothesized stronger cellulose binding would result in greater wall‐loosening activity and used in vitro evolution of Bacillus subtilis BsEXLX1 to test this hypothesis. Mutants with stronger binding generally had greater wall‐loosening activity, but the relationship was nonlinear and plateaued at ~ 40% higher than wild‐type. Mutant E191K exhibited stronger cellulose binding but failed to induce creep, evidently due to protein mistargeting. These results reveal the complexity of interactions between plant cell walls and wall‐modifying proteins, an important consideration when engineering proteins for applications in biofuel production and plant pathogen resistance." @default.
• W2953452020 created "2019-07-12" @default.
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• W2953452020 creator A5057487274 @default.
• W2953452020 date "2019-07-19" @default.
• W2953452020 modified "2023-09-23" @default.
• W2953452020 title "Directed <i>in vitro</i> evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall‐loosening activities" @default.
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• W2953452020 doi "https://doi.org/10.1002/1873-3468.13528" @default.
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