FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953470147> ?p ?o ?g. }

• W2953470147 endingPage "728" @default.
• W2953470147 startingPage "724" @default.
• W2953470147 abstract "1. In order to increase the efficiency of generating transgenic chicken, this trial focused on two points: primordial germ cells (PGCs）transfection in vivo and a germline-specific promoter.2. In order to transfect PGCs in vivo, two plasmids (pZB-CAG-GFP, pCMV-ZB）were co-injected into chicken embryos via the subgerminal cavity at Hamburger and Hamilton (HH) stage 2–3 or via blood vessel at HH stage 13–14. Results showed that the percentage of GFP+ embryos, viability and hatching rate of embryos injected at HH stage 13–14 were significantly higher than that at HH stage 2–3.3. Two plasmid transposon systems were used for chicken embryo micro-injections. The donor plasmid, with a green fluorescent protein (GFP) reporter gene, was mediated by the ZB transposon. The helper plasmid was a transposase expression vector driven by the promoter of the chicken vasa homologue (Cvh) gene or Human cytomegalovirus (CMV) promoter. Results showed that 60.98% of gonads in Cvh group expressed GFP, which was 52.50% higher than seen in the CMV group. Only gonad tissue from the Cvh group showed any GFP signal, whereas both gonads and other tissues in the CMV group showed green fluorescence.4. The data suggested that ZB transposon-mediated gene transfer was efficient for transfecting PGCs in vivo; the Cvh promoter drove the transposase gene specifically in the germline and increased the efficiency of germline transmission. Blood vessels injection at HH stage 13–14 may be a more efficient route for PGCs transfection in vivo." @default.
• W2953470147 created "2019-07-12" @default.
• W2953470147 creator A5000489490 @default.
• W2953470147 creator A5002699262 @default.
• W2953470147 creator A5004808253 @default.
• W2953470147 creator A5044578163 @default.
• W2953470147 creator A5051041351 @default.
• W2953470147 creator A5052618328 @default.
• W2953470147 creator A5059402212 @default.
• W2953470147 creator A5069306557 @default.
• W2953470147 creator A5073815972 @default.
• W2953470147 date "2019-07-29" @default.
• W2953470147 modified "2023-09-26" @default.
• W2953470147 title "ZB transposon and chicken vasa homologue (Cvh) promoter interact to increase transfection efficiency of primordial germ cells<i>in vivo</i>" @default.
• W2953470147 cites W1977269173 @default.
• W2953470147 cites W1988064554 @default.
• W2953470147 cites W1994921625 @default.
• W2953470147 cites W2021290198 @default.
• W2953470147 cites W2030393062 @default.
• W2953470147 cites W2039751785 @default.
• W2953470147 cites W2083961776 @default.
• W2953470147 cites W2155793482 @default.
• W2953470147 cites W2164198084 @default.
• W2953470147 cites W2164618384 @default.
• W2953470147 cites W2293359325 @default.
• W2953470147 cites W4230725901 @default.
• W2953470147 cites W4253075439 @default.
• W2953470147 doi "https://doi.org/10.1080/00071668.2019.1639138" @default.
• W2953470147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31267766" @default.
• W2953470147 hasPublicationYear "2019" @default.
• W2953470147 type Work @default.
• W2953470147 sameAs 2953470147 @default.
• W2953470147 citedByCount "2" @default.
• W2953470147 countsByYear W29534701472020 @default.
• W2953470147 countsByYear W29534701472022 @default.
• W2953470147 crossrefType "journal-article" @default.
• W2953470147 hasAuthorship W2953470147A5000489490 @default.
• W2953470147 hasAuthorship W2953470147A5002699262 @default.
• W2953470147 hasAuthorship W2953470147A5004808253 @default.
• W2953470147 hasAuthorship W2953470147A5044578163 @default.
• W2953470147 hasAuthorship W2953470147A5051041351 @default.
• W2953470147 hasAuthorship W2953470147A5052618328 @default.
• W2953470147 hasAuthorship W2953470147A5059402212 @default.
• W2953470147 hasAuthorship W2953470147A5069306557 @default.
• W2953470147 hasAuthorship W2953470147A5073815972 @default.
• W2953470147 hasConcept C102230213 @default.
• W2953470147 hasConcept C104317684 @default.
• W2953470147 hasConcept C109825262 @default.
• W2953470147 hasConcept C142613039 @default.
• W2953470147 hasConcept C143065580 @default.
• W2953470147 hasConcept C150194340 @default.
• W2953470147 hasConcept C153911025 @default.
• W2953470147 hasConcept C161733203 @default.
• W2953470147 hasConcept C172510086 @default.
• W2953470147 hasConcept C196843134 @default.
• W2953470147 hasConcept C207001950 @default.
• W2953470147 hasConcept C22744801 @default.
• W2953470147 hasConcept C4918238 @default.
• W2953470147 hasConcept C54009773 @default.
• W2953470147 hasConcept C54355233 @default.
• W2953470147 hasConcept C86803240 @default.
• W2953470147 hasConcept C95444343 @default.
• W2953470147 hasConceptScore W2953470147C102230213 @default.
• W2953470147 hasConceptScore W2953470147C104317684 @default.
• W2953470147 hasConceptScore W2953470147C109825262 @default.
• W2953470147 hasConceptScore W2953470147C142613039 @default.
• W2953470147 hasConceptScore W2953470147C143065580 @default.
• W2953470147 hasConceptScore W2953470147C150194340 @default.
• W2953470147 hasConceptScore W2953470147C153911025 @default.
• W2953470147 hasConceptScore W2953470147C161733203 @default.
• W2953470147 hasConceptScore W2953470147C172510086 @default.
• W2953470147 hasConceptScore W2953470147C196843134 @default.
• W2953470147 hasConceptScore W2953470147C207001950 @default.
• W2953470147 hasConceptScore W2953470147C22744801 @default.
• W2953470147 hasConceptScore W2953470147C4918238 @default.
• W2953470147 hasConceptScore W2953470147C54009773 @default.
• W2953470147 hasConceptScore W2953470147C54355233 @default.
• W2953470147 hasConceptScore W2953470147C86803240 @default.
• W2953470147 hasConceptScore W2953470147C95444343 @default.
• W2953470147 hasFunder F4320321001 @default.
• W2953470147 hasIssue "6" @default.
• W2953470147 hasLocation W29534701471 @default.
• W2953470147 hasLocation W29534701472 @default.
• W2953470147 hasOpenAccess W2953470147 @default.
• W2953470147 hasPrimaryLocation W29534701471 @default.
• W2953470147 hasRelatedWork W1967691623 @default.
• W2953470147 hasRelatedWork W1983069895 @default.
• W2953470147 hasRelatedWork W2008114314 @default.
• W2953470147 hasRelatedWork W2038578692 @default.
• W2953470147 hasRelatedWork W2076870585 @default.
• W2953470147 hasRelatedWork W2086105540 @default.
• W2953470147 hasRelatedWork W2113016186 @default.
• W2953470147 hasRelatedWork W2157545440 @default.
• W2953470147 hasRelatedWork W2413743288 @default.
• W2953470147 hasRelatedWork W3151964627 @default.
• W2953470147 hasVolume "60" @default.
• W2953470147 isParatext "false" @default.
• W2953470147 isRetracted "false" @default.

• next