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- W2953555886 abstract "Peste des petits ruminants (PPR) is an economically important, highly contagious viral disease of sheep and goats characterized by high morbidity and mortality rates. A live attenuated PPR vaccine has been produced in Veterinary Serum and Vaccines Research Institute (VSVRI), Abbasia, Cairo, Egypt. It has and still been utilized to control PPR disease in endemic areas of the Arabian Gulf. In this study, the identity of four batches of PPR vaccines was tested as per the Office International des Epizooties (OIE) guidelines using RT-PCR technique that based on the amplification of fragments of F-protein and fragments of N-protein. All four batches are also tested for identity using real time RT-PCR. Results in this study showed that molecular techniques could be used for rapid evaluation of PPR vaccine including RT-PCR and real time RT-PCR for identity testing. However, the one-step real-time RT-PCR is proved to be the most rapid, sensitive and specific assay for identification of peste des petits ruminants virus in peste des petits ruminants (PPR) vaccines. In the meanwhile, the conventional RT-PCR using primer set that targets N gene is more sensitive in identification of PPRV than RT-PCR using primer set that targets F gene which could give false negative results." @default.
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- W2953555886 date "2017-01-01" @default.
- W2953555886 modified "2023-09-27" @default.
- W2953555886 title "Some molecular assays used for identity of Peste des petits ruminants (PPR) vaccines" @default.
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