Matches in SemOpenAlex for { <https://semopenalex.org/work/W2953646530> ?p ?o ?g. }
- W2953646530 endingPage "e0219163" @default.
- W2953646530 startingPage "e0219163" @default.
- W2953646530 abstract "Non-typhoidal Salmonella enterica serovars continue to be an important food safety issue worldwide. Cranberry (Vaccinium macrocarpon Ait) fruits possess antimicrobial properties due to their various acids and phenolic compounds; however, the underlying mechanism of actions is poorly understood. We evaluated the effects of cranberry extracts on the growth rate of Salmonella enterica serovars Typhimurium, Enteritidis and Heidelberg and on the transcriptomic profile of Salmonella Enteritidis to gain insight into phenotypic and transcriptional changes induced by cranberry extracts on this pathogen. An ethanolic extract from cranberry pomaces (KCOH) and two of its sub-fractions, anthocyanins (CRFa20) and non-anthocyanin polyphenols (CRFp85), were used. The minimum inhibitory (MICs) and bactericidal (MBCs) concentrations of these fractions against tested pathogens were obtained using the broth micro-dilution method according to the Clinical Laboratory Standard Institute’s guidelines. Transcriptional profiles of S. Enteritidis grown in cation-adjusted Mueller-Hinton broth supplemented with or without 2 or 4 mg/ml of KCOH were compared by RNASeq to reveal gene modulations serving as markers for biological activity. The MIC and MBC values of KCOH were 8 and 16 mg/mL, respectively, against all tested S. enterica isolates. The MIC value was 4 mg/mL for both CRFa20 and CRFp85 sub-fractions, and a reduced MBC value was obtained for CRFp85 (4 mg/ml). Treatment of S. Enteritidis with KCOH revealed a concentration-dependent transcriptional signature. Compared to the control, 2 mg/ml of KCOH exposure resulted in 89 differentially expressed genes (DEGs), of which 53 and 36 were downregulated and upregulated, respectively. The upregulated genes included those involved in citrate metabolism, enterobactin synthesis and transport, and virulence. Exposure to 4 mg/ml KCOH led to the modulated expression of 376 genes, of which 233 were downregulated and 143 upregulated, which is 4.2 times more DEGs than from exposure to 2 mg/ml KCOH. The downregulated genes were related to flagellar motility, Salmonella Pathogenicity Island-1 (SPI-1), cell wall/membrane biogenesis, and transcription. Moreover, genes involved in energy production and conversion, carbohydrate transport and metabolism, and coenzyme transport and metabolism were upregulated during exposure to 4 mg/ml KCOH. Overall, 57 genes were differentially expressed (48 downregulated and 9 upregulated) in response to both concentrations. Both concentrations of KCOH downregulated expression of hilA, which is a major SPI-1 transcriptional regulator. This study provides information on the response of Salmonella exposed to cranberry extracts, which could be used in the control of this important foodborne pathogen." @default.
- W2953646530 created "2019-07-12" @default.
- W2953646530 creator A5009770102 @default.
- W2953646530 creator A5037425173 @default.
- W2953646530 creator A5045472476 @default.
- W2953646530 creator A5061435774 @default.
- W2953646530 creator A5073239759 @default.
- W2953646530 creator A5078764103 @default.
- W2953646530 creator A5080029468 @default.
- W2953646530 creator A5088861827 @default.
- W2953646530 date "2019-07-03" @default.
- W2953646530 modified "2023-10-15" @default.
- W2953646530 title "Transcriptional profiling of Salmonella enterica serovar Enteritidis exposed to ethanolic extract of organic cranberry pomace" @default.
- W2953646530 cites W12707783 @default.
- W2953646530 cites W1537021148 @default.
- W2953646530 cites W1543337462 @default.
- W2953646530 cites W1824628255 @default.
- W2953646530 cites W1902901837 @default.
- W2953646530 cites W1969504421 @default.
- W2953646530 cites W1975606159 @default.
- W2953646530 cites W1989274500 @default.
- W2953646530 cites W2000714993 @default.
- W2953646530 cites W2005091504 @default.
- W2953646530 cites W2008788566 @default.
- W2953646530 cites W2015072389 @default.
- W2953646530 cites W2028204615 @default.
- W2953646530 cites W2030071253 @default.
- W2953646530 cites W2045753901 @default.
- W2953646530 cites W2063570013 @default.
- W2953646530 cites W2071980499 @default.
- W2953646530 cites W2078429267 @default.
- W2953646530 cites W2081992825 @default.
- W2953646530 cites W2082753669 @default.
- W2953646530 cites W2084870117 @default.
- W2953646530 cites W2097326265 @default.
- W2953646530 cites W2114104545 @default.
- W2953646530 cites W2115496478 @default.
- W2953646530 cites W2122487467 @default.
- W2953646530 cites W2131271579 @default.
- W2953646530 cites W2134526812 @default.
- W2953646530 cites W2136632285 @default.
- W2953646530 cites W2138271600 @default.
- W2953646530 cites W2142214919 @default.
- W2953646530 cites W2149999650 @default.
- W2953646530 cites W2150868016 @default.
- W2953646530 cites W2160379773 @default.
- W2953646530 cites W2166826452 @default.
- W2953646530 cites W2168416849 @default.
- W2953646530 cites W2171865415 @default.
- W2953646530 cites W2249732093 @default.
- W2953646530 cites W2336123775 @default.
- W2953646530 cites W2488509250 @default.
- W2953646530 cites W2514491726 @default.
- W2953646530 cites W2519628423 @default.
- W2953646530 cites W2527743647 @default.
- W2953646530 cites W2555835127 @default.
- W2953646530 cites W2594469169 @default.
- W2953646530 cites W2751101409 @default.
- W2953646530 cites W2795549399 @default.
- W2953646530 cites W2830281350 @default.
- W2953646530 cites W2903394252 @default.
- W2953646530 cites W2905087251 @default.
- W2953646530 cites W2923647077 @default.
- W2953646530 cites W3011627431 @default.
- W2953646530 cites W4243245296 @default.
- W2953646530 cites W4298020519 @default.
- W2953646530 doi "https://doi.org/10.1371/journal.pone.0219163" @default.
- W2953646530 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6608956" @default.
- W2953646530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31269043" @default.
- W2953646530 hasPublicationYear "2019" @default.
- W2953646530 type Work @default.
- W2953646530 sameAs 2953646530 @default.
- W2953646530 citedByCount "23" @default.
- W2953646530 countsByYear W29536465302020 @default.
- W2953646530 countsByYear W29536465302021 @default.
- W2953646530 countsByYear W29536465302022 @default.
- W2953646530 countsByYear W29536465302023 @default.
- W2953646530 crossrefType "journal-article" @default.
- W2953646530 hasAuthorship W2953646530A5009770102 @default.
- W2953646530 hasAuthorship W2953646530A5037425173 @default.
- W2953646530 hasAuthorship W2953646530A5045472476 @default.
- W2953646530 hasAuthorship W2953646530A5061435774 @default.
- W2953646530 hasAuthorship W2953646530A5073239759 @default.
- W2953646530 hasAuthorship W2953646530A5078764103 @default.
- W2953646530 hasAuthorship W2953646530A5080029468 @default.
- W2953646530 hasAuthorship W2953646530A5088861827 @default.
- W2953646530 hasBestOaLocation W29536465301 @default.
- W2953646530 hasConcept C104317684 @default.
- W2953646530 hasConcept C150194340 @default.
- W2953646530 hasConcept C162317418 @default.
- W2953646530 hasConcept C176947019 @default.
- W2953646530 hasConcept C185592680 @default.
- W2953646530 hasConcept C2778004101 @default.
- W2953646530 hasConcept C2778945092 @default.
- W2953646530 hasConcept C2779607309 @default.
- W2953646530 hasConcept C2780950330 @default.
- W2953646530 hasConcept C2781065037 @default.
- W2953646530 hasConcept C2992956773 @default.