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• W2953663843 abstract "The soluble interleukin-2 receptor α (sIL-2Rα) is a broad indicator of clinical disease activity in various inflammatory diseases. Here we have developed, for the first time, a rapid, washing-free colorimetric aptasensor based on a sIL-2Rα aptamer (Kd=1.33 nm). The aptasensor was fabricated with Au nanoparticles (AuNPs) adsorbing sIL-2Rα aptamers. On addition of sIL-2Rα, the aptamers become desorbed from the AuNPs, and this in turn weakens the absorption corresponding to AuNP-catalyzed oxidation of ortho-phenylenediamine (oPD) with H2O2. The aptasensor was characterized by TEM imaging, ζ potential measurements, dynamic light scattering (DLS) analysis, and UV/Vis spectrometry, followed by further optimization. The fabricated sensor exhibited great analytical performance, with a linear range of 1 to 100 nm and a detection limit of 1 nm both in buffer and in spiked human serum within 25 min. Other proteins, such as bovine serum albumin (BSA), IL-17Rα, IL-5Rα, IL-13Rα2, and CD166, showed negligible effects on the aptasensor. Thanks to the great advantages of the aptamers and AuNPs, this aptasensor provides a rapid, simple, and inexpensive process that might offer insights into various diagnostic applications of sIL-2Rα." @default.
• W2953663843 created "2019-07-12" @default.
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• W2953663843 date "2019-07-04" @default.
• W2953663843 modified "2023-10-10" @default.
• W2953663843 title "A Rapid Colorimetric Sensor for Soluble Interleukin‐2 Receptor α, Based on Aptamer‐Adsorbed AuNP" @default.
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• W2953663843 doi "https://doi.org/10.1002/cbic.201900109" @default.
• W2953663843 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31270926" @default.
• W2953663843 hasPublicationYear "2019" @default.
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