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• W2953670082 abstract "As an effective and well-recognized strategy used in many delivery systems, such as polycation gene vectors, charge reversal refers to the alternation of vector surface charge from negative (in blood circulation) to positive (in the targeted tissue) in response to specific stimuli to simultaneously satisfy the requirements of biocompatibility and targeting. Although charge reversal vectors are intended to avoid interactions with blood in their application, no overall or systematic investigation has been carried out to verify the role of charge reversal in the blood compatibility. Herein, we comprehensively mapped the effects of a typical charge-reversible polycation gene vector based on pH-responsive 2,3-dimethylmaleic anhydride (DMMA)-modified polyethylenimine (PEI)/pDNA complex in terms of blood components, coagulation function, and immune response as compared to conventional PEGylated modification. The in vitro and in vivo results displayed that charge-reversal modification significantly improves the PEI/pDNA-induced abnormal effect on vascular endothelial cells, platelet activation, clotting factor activity, fibrinogen polymerization, blood coagulation process, and pro-inflammatory cytokine expression. Unexpectedly, (PEI/pDNA)-DMMA induced the cytoskeleton impairment-mediated erythrocyte morphological alternation and complement activation even more than PEI/pDNA. Further, transcriptome sequencing demonstrated that the overexpression of pro-inflammatory cytokines was correlated with vector-induced differentially expressed gene number and mediated by inflammation-related signaling pathways (MAPK, NF-κB, Toll-like receptor, and JAK-STAT) activation. By comparison, charge-reversal modification improved the hemocompatibility to a greater extent than dose PEGylation except for erythrocyte rupture. Nevertheless, it is inferior to mPEG modification in terms of immunocompatibility. These findings provide comprehensive insights to understand the molecular mechanisms of the effects of charge reversal on blood components and their function and to provide valuable information for its potential applications from laboratory to clinic. The seemingly revolutionary charge reversal strategy has been believed to possess stealth character with negative charge eluding interaction with blood components during circulation. However to date, no overall or systematic investigation has been carried out to verify the role of charge-reversal on the blood/immune compatibility, which impede their development from laboratory to bedside. Therefore, we comprehensively mapped the effects of a typical charge-reversible polycationic gene vector on blood components (vascular endothelial cell, platelet, clotting factors, fibrinogen, RBCs and coagulation function) and immune response (complement and pro-inflammatory cytokines) at cellular and molecular level in comparison to PEGylation modification. These findings help to elucidate the molecular mechanisms for the effects of charge-reversal on blood components and functions, and provide valuable information for the possible application in clinical settings." @default.
• W2953670082 created "2019-07-12" @default.
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• W2953670082 date "2019-09-01" @default.
• W2953670082 modified "2023-10-06" @default.
• W2953670082 title "Role of charge-reversal in the hemo/immuno-compatibility of polycationic gene delivery systems" @default.
• W2953670082 cites W1187007897 @default.
• W2953670082 cites W1911744880 @default.
• W2953670082 cites W1966218664 @default.
• W2953670082 cites W1969951819 @default.
• W2953670082 cites W1975603353 @default.
• W2953670082 cites W1980671882 @default.
• W2953670082 cites W1987665445 @default.
• W2953670082 cites W1989444997 @default.
• W2953670082 cites W1996098588 @default.
• W2953670082 cites W1998348474 @default.
• W2953670082 cites W2001079236 @default.
• W2953670082 cites W2001617694 @default.
• W2953670082 cites W2007513387 @default.
• W2953670082 cites W2009894847 @default.
• W2953670082 cites W2024738645 @default.
• W2953670082 cites W2025193872 @default.
• W2953670082 cites W2025990308 @default.
• W2953670082 cites W2026257148 @default.
• W2953670082 cites W2036816204 @default.
• W2953670082 cites W2038278541 @default.
• W2953670082 cites W2040053115 @default.
• W2953670082 cites W2042208046 @default.
• W2953670082 cites W2044626782 @default.
• W2953670082 cites W2045857341 @default.
• W2953670082 cites W2047668623 @default.
• W2953670082 cites W2053383601 @default.
• W2953670082 cites W2054045589 @default.
• W2953670082 cites W2055434207 @default.
• W2953670082 cites W2056311063 @default.
• W2953670082 cites W2056645396 @default.
• W2953670082 cites W2061695565 @default.
• W2953670082 cites W2064920706 @default.
• W2953670082 cites W2080091719 @default.
• W2953670082 cites W2083175247 @default.
• W2953670082 cites W2083466958 @default.
• W2953670082 cites W2085163663 @default.
• W2953670082 cites W2092319622 @default.
• W2953670082 cites W2109477306 @default.
• W2953670082 cites W2114104545 @default.
• W2953670082 cites W2121781418 @default.
• W2953670082 cites W2131888732 @default.
• W2953670082 cites W2138485536 @default.
• W2953670082 cites W2144350652 @default.
• W2953670082 cites W2145169943 @default.
• W2953670082 cites W2159227890 @default.
• W2953670082 cites W2167933060 @default.
• W2953670082 cites W2170885324 @default.
• W2953670082 cites W2298468466 @default.
• W2953670082 cites W2301650719 @default.
• W2953670082 cites W2314799819 @default.
• W2953670082 cites W2323895118 @default.
• W2953670082 cites W2331408352 @default.
• W2953670082 cites W2339751706 @default.
• W2953670082 cites W2504945737 @default.
• W2953670082 cites W2535295987 @default.
• W2953670082 cites W2566835558 @default.
• W2953670082 cites W2581974543 @default.
• W2953670082 cites W2589800990 @default.
• W2953670082 cites W2611647652 @default.
• W2953670082 cites W2765754868 @default.
• W2953670082 cites W2770162756 @default.
• W2953670082 cites W2774216383 @default.
• W2953670082 cites W2787426757 @default.
• W2953670082 cites W2802198795 @default.
• W2953670082 cites W2804065022 @default.
• W2953670082 cites W2805861692 @default.
• W2953670082 cites W2806460138 @default.
• W2953670082 cites W2913761453 @default.
• W2953670082 doi "https://doi.org/10.1016/j.actbio.2019.06.043" @default.
• W2953670082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31254682" @default.
• W2953670082 hasPublicationYear "2019" @default.
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