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- W2953717599 abstract "Rhamnolipids (RLs) are glycolipidic compounds produced by a few of bacterial species, especially Pseudomonasand Burkholderia spp. These compounds display excellent surfactant properties and environmentaladvantages. Nevertheless, their high production cost hampers their practical use in industry. Inaddition, bacterial strains that produce RLs generate a mixture of congeners with varying lipophilicchain lengths, therefore affecting their macromolecular behavior. Since the physicochemical characteristicsof RLs are directly influenced by their molecular structure, modification or improvement of their surfactantproperties can be achieved by controlling the length of their alkyl chains. RhlA acts as a keyenzyme in the RL biosynthesis pathway. The enzyme catalyzes the esterification reaction between twounits of hydroxylated fatty acids to form a dimer, s-3-(3-hydroxyalkanoyl) alkanoic acid (HAA), the dilipidprecursor of RLs. HAA biosynthesis is the rate-limiting step in RL biosynthesis. Here, we present asemi-rational evolution approach to engineer RhlA from P. aeruginosa to increase in vivo HAA productionand to modulate substrate selectivity. Using a structural homology model of the enzyme, we predicteda number of substrate-interacting residues and performed intragenic suppression-typemutagenesis to increase the catalytic efficiency of RhlA. We also employed a chimeric approach to identifyprotein domains involved in enzyme selectivity, in addition to performing site-directed mutagenesison residues located in the substrate binding pocket to modulate RL congener biosynthesis. Our resultsprovide evidence that protein engineering approaches can be efficiently employed to improve RL productionin P. aeruginosa." @default.
- W2953717599 created "2019-07-12" @default.
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- W2953717599 date "2017-01-01" @default.
- W2953717599 modified "2023-09-27" @default.
- W2953717599 title "Semi-rational evolution of the Pseudomonas aeruginosa Rhamnosyltransferase 1 subunit A (RhlA) for the synthesis of industrially relevant rhamnolipids" @default.
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