Matches in SemOpenAlex for { <https://semopenalex.org/work/W2954028412> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2954028412 abstract "Author(s): Sos, Brandon | Advisor(s): Zhang, Kun | Abstract: Chromatin accessibility captures in vivo protein-chromosome binding status, and is considered an informative proxy for protein-DNA interactions. DNase I and Tn5 transposase assays require thousands to millions of fresh cells for comprehensive chromatin mapping. Applying Tn5 tagmentation to hundreds of cells and to single cells results in sparse chromatin maps at high throughput or dense chromatin maps at low throughput. We present a high throughput transposome hypersensitive sites sequencing assay for highly sensitive characterization of chromatin accessibility. Linear amplification of accessible DNA ends with in vitro transcription, coupled with an engineered Tn5 super-mutant, demonstrates improved sensitivity on limited input materials, and accessibility of small regions near distal enhancers, compared with ATAC-seq. Application to single cells was achieved by adapting THS-seq to combinatorial indexing to generate high sensitivity DNA accessibility maps for tens of thousands of single cells from the human adult visual and frontal cortex. Integrative analysis of snDrop-seq and scTHS-seq has allowed us to identify transcription factors and regulatory elements shaping the state of different brain cell types, and to map genetic risk factors of common human brain diseases to specific pathogenic cell types and subtypes. Overall we demonstrate the viability of scTHS-seq to quantitate DNA accessibility in single cells, and application to biological samples to shed insight on human brain diseases." @default.
- W2954028412 created "2019-07-12" @default.
- W2954028412 creator A5057223773 @default.
- W2954028412 date "2017-01-01" @default.
- W2954028412 modified "2023-09-27" @default.
- W2954028412 title "The Single Cell Transposome Hypersensitive Sites Sequencing (scTHS-seq) assay for Chromatin Accessibility and Assessment of Epigenetic States in the Human Adult Brain" @default.
- W2954028412 hasPublicationYear "2017" @default.
- W2954028412 type Work @default.
- W2954028412 sameAs 2954028412 @default.
- W2954028412 citedByCount "0" @default.
- W2954028412 crossrefType "journal-article" @default.
- W2954028412 hasAuthorship W2954028412A5057223773 @default.
- W2954028412 hasConcept C104317684 @default.
- W2954028412 hasConcept C111936080 @default.
- W2954028412 hasConcept C167227067 @default.
- W2954028412 hasConcept C41091548 @default.
- W2954028412 hasConcept C54355233 @default.
- W2954028412 hasConcept C552990157 @default.
- W2954028412 hasConcept C67258246 @default.
- W2954028412 hasConcept C70721500 @default.
- W2954028412 hasConcept C83640560 @default.
- W2954028412 hasConcept C86339819 @default.
- W2954028412 hasConcept C86803240 @default.
- W2954028412 hasConcept C93946551 @default.
- W2954028412 hasConcept C95444343 @default.
- W2954028412 hasConceptScore W2954028412C104317684 @default.
- W2954028412 hasConceptScore W2954028412C111936080 @default.
- W2954028412 hasConceptScore W2954028412C167227067 @default.
- W2954028412 hasConceptScore W2954028412C41091548 @default.
- W2954028412 hasConceptScore W2954028412C54355233 @default.
- W2954028412 hasConceptScore W2954028412C552990157 @default.
- W2954028412 hasConceptScore W2954028412C67258246 @default.
- W2954028412 hasConceptScore W2954028412C70721500 @default.
- W2954028412 hasConceptScore W2954028412C83640560 @default.
- W2954028412 hasConceptScore W2954028412C86339819 @default.
- W2954028412 hasConceptScore W2954028412C86803240 @default.
- W2954028412 hasConceptScore W2954028412C93946551 @default.
- W2954028412 hasConceptScore W2954028412C95444343 @default.
- W2954028412 hasLocation W29540284121 @default.
- W2954028412 hasOpenAccess W2954028412 @default.
- W2954028412 hasPrimaryLocation W29540284121 @default.
- W2954028412 hasRelatedWork W2159103435 @default.
- W2954028412 hasRelatedWork W2463512177 @default.
- W2954028412 hasRelatedWork W2472955106 @default.
- W2954028412 hasRelatedWork W2746829434 @default.
- W2954028412 hasRelatedWork W2767145741 @default.
- W2954028412 hasRelatedWork W2799147145 @default.
- W2954028412 hasRelatedWork W2896445416 @default.
- W2954028412 hasRelatedWork W2901586501 @default.
- W2954028412 hasRelatedWork W2921996670 @default.
- W2954028412 hasRelatedWork W2948332103 @default.
- W2954028412 hasRelatedWork W2949426619 @default.
- W2954028412 hasRelatedWork W3000046370 @default.
- W2954028412 hasRelatedWork W3016715268 @default.
- W2954028412 hasRelatedWork W3016866540 @default.
- W2954028412 hasRelatedWork W3080090219 @default.
- W2954028412 hasRelatedWork W3084200104 @default.
- W2954028412 hasRelatedWork W3116849561 @default.
- W2954028412 hasRelatedWork W3119669192 @default.
- W2954028412 hasRelatedWork W3164839005 @default.
- W2954028412 hasRelatedWork W3186945407 @default.
- W2954028412 isParatext "false" @default.
- W2954028412 isRetracted "false" @default.
- W2954028412 magId "2954028412" @default.
- W2954028412 workType "article" @default.