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- W2954201711 abstract "In the present study, the polycaprolactone (PCL) nanofibers were investigated as a carrier to deliver phytochemicals for bone and cartilage tissue engineering. The PCL nanofibers was blended with phytochemicals hexadecanoic acid, octadecanoic acid and N , N -diisopropyl (2,2,3,3,3-pentafluoropropyl) amine isolated from a medicinal plant, Wattakaka volubilis . The scaffolds were characterized using scanning electron microscope (SEM) and Fourier transform infrared (FTIR) spectroscopy. The average diameter of control and phytochemical loaded nanofiber was 208 ± 9.6 nm and 316 ± 7.0 nm respectively. Biodegradation rate of nanofibers, impact of nanofiber on meniscus and osteoblast cell growth was analyzed using 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) assay, DNA content and extra cellular matrix secretion. Hoechst stain and SEM images were used to visualize and monitor the cell growth on PCL scaffold. The phytochemicals incorporated PCL nanofibers enhanced the growth and proliferation of primary human meniscus and osteoblast like cells and hence may be suitable scaffold for bone and cartilage tissue engineering applications. Wattakaka volubilis active phytochemicals impregnated PCL nanofibers for primary human meniscus and MG63 osteoblast cell proliferation. • Selected phytochemicals of W. volubilis leaf were coelectrospun with PCL as nanofiber. • The phytochemical loaded nanofiber enhanced the growth and proliferation of human primary human meniscus cells and MG63 osteoblast like cells. • Phytochemical loaded nanofibers was nontoxic and enhanced DNA content, extracellular matrix production. • Phytochemical loaded nanofiber can be used as scaffold for bone and cartilage tissue engineering." @default.
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- W2954201711 date "2019-10-01" @default.
- W2954201711 modified "2023-09-26" @default.
- W2954201711 title "Electrospun PCL nanofibers blended with Wattakaka volubilis active phytochemicals for bone and cartilage tissue engineering" @default.
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- W2954201711 doi "https://doi.org/10.1016/j.nano.2019.102044" @default.
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