FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2954246512> ?p ?o ?g. }

• W2954246512 endingPage "11922" @default.
• W2954246512 startingPage "11912" @default.
• W2954246512 abstract "Small molecule inhibitors often only block a subset of the cellular functions of their protein targets. In many cases, how inhibiting only a portion of a multifunctional protein’s functions affects the state of the cell is not well-understood. Therefore, tools that allow the systematic characterization of the cellular interactions that inhibitor-bound proteins make would be of great utility, especially for multifunctional proteins. Here, we describe a chemoproteomic strategy for interrogating the cellular localization and interactomes of inhibitor-bound kinases. By developing a set of orthogonal inhibitors that contain a trans-cyclooctene (TCO) click handle, we are able to enrich and characterize the proteins complexed to a drug-sensitized variant of the multidomain kinase Src. We show that Src’s cellular interactions are highly influenced by the intermolecular accessibility of its regulatory domains, which can be allosterically modulated through its ATP-binding site. Furthermore, we find that the signaling status of the cell also has a large effect on Src’s interactome. Finally, we demonstrate that our TCO-conjugated probes can be used as a part of a proximity ligation assay to study Src’s localization and interactions in situ. Together, our chemoproteomic strategy represents a comprehensive method for studying the localization and interactomes of inhibitor-bound kinases and, potentially, other druggable protein targets." @default.
• W2954246512 created "2019-07-12" @default.
• W2954246512 creator A5003432548 @default.
• W2954246512 creator A5016646503 @default.
• W2954246512 creator A5032347056 @default.
• W2954246512 creator A5054397869 @default.
• W2954246512 creator A5067945060 @default.
• W2954246512 creator A5068023412 @default.
• W2954246512 date "2019-06-24" @default.
• W2954246512 modified "2023-09-27" @default.
• W2954246512 title "Chemoproteomic Method for Profiling Inhibitor-Bound Kinase Complexes" @default.
• W2954246512 cites W1521881285 @default.
• W2954246512 cites W1526377515 @default.
• W2954246512 cites W1549772491 @default.
• W2954246512 cites W1560020441 @default.
• W2954246512 cites W1897425543 @default.
• W2954246512 cites W1973883065 @default.
• W2954246512 cites W1980422087 @default.
• W2954246512 cites W1981783595 @default.
• W2954246512 cites W1992496728 @default.
• W2954246512 cites W1997099073 @default.
• W2954246512 cites W2010995825 @default.
• W2954246512 cites W2013821696 @default.
• W2954246512 cites W2014557438 @default.
• W2954246512 cites W2019792957 @default.
• W2954246512 cites W2022095303 @default.
• W2954246512 cites W2030205108 @default.
• W2954246512 cites W2030953184 @default.
• W2954246512 cites W2048072131 @default.
• W2954246512 cites W2051165598 @default.
• W2954246512 cites W2053582875 @default.
• W2954246512 cites W2053656371 @default.
• W2954246512 cites W2061324224 @default.
• W2954246512 cites W2062494005 @default.
• W2954246512 cites W2062524026 @default.
• W2954246512 cites W2064990000 @default.
• W2954246512 cites W2069180517 @default.
• W2954246512 cites W2075820316 @default.
• W2954246512 cites W2080752012 @default.
• W2954246512 cites W2088659758 @default.
• W2954246512 cites W2093913323 @default.
• W2954246512 cites W2096173332 @default.
• W2954246512 cites W2103017472 @default.
• W2954246512 cites W2126761758 @default.
• W2954246512 cites W2142358502 @default.
• W2954246512 cites W2149554987 @default.
• W2954246512 cites W2153977062 @default.
• W2954246512 cites W2156719482 @default.
• W2954246512 cites W2225583487 @default.
• W2954246512 cites W2266679693 @default.
• W2954246512 cites W2314815212 @default.
• W2954246512 cites W2317007660 @default.
• W2954246512 cites W2406605691 @default.
• W2954246512 cites W2463195069 @default.
• W2954246512 cites W2465035186 @default.
• W2954246512 cites W2537623931 @default.
• W2954246512 cites W2540583676 @default.
• W2954246512 cites W2604808360 @default.
• W2954246512 cites W2665188720 @default.
• W2954246512 cites W2770101695 @default.
• W2954246512 cites W2932287678 @default.
• W2954246512 cites W41188765 @default.
• W2954246512 doi "https://doi.org/10.1021/jacs.9b02963" @default.
• W2954246512 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6688853" @default.
• W2954246512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31274292" @default.
• W2954246512 hasPublicationYear "2019" @default.
• W2954246512 type Work @default.
• W2954246512 sameAs 2954246512 @default.
• W2954246512 citedByCount "10" @default.
• W2954246512 countsByYear W29542465122019 @default.
• W2954246512 countsByYear W29542465122020 @default.
• W2954246512 countsByYear W29542465122021 @default.
• W2954246512 countsByYear W29542465122022 @default.
• W2954246512 countsByYear W29542465122023 @default.
• W2954246512 crossrefType "journal-article" @default.
• W2954246512 hasAuthorship W2954246512A5003432548 @default.
• W2954246512 hasAuthorship W2954246512A5016646503 @default.
• W2954246512 hasAuthorship W2954246512A5032347056 @default.
• W2954246512 hasAuthorship W2954246512A5054397869 @default.
• W2954246512 hasAuthorship W2954246512A5067945060 @default.
• W2954246512 hasAuthorship W2954246512A5068023412 @default.
• W2954246512 hasBestOaLocation W29542465122 @default.
• W2954246512 hasConcept C104317684 @default.
• W2954246512 hasConcept C10679952 @default.
• W2954246512 hasConcept C108636557 @default.
• W2954246512 hasConcept C11804247 @default.
• W2954246512 hasConcept C161624437 @default.
• W2954246512 hasConcept C184235292 @default.
• W2954246512 hasConcept C185592680 @default.
• W2954246512 hasConcept C46111723 @default.
• W2954246512 hasConcept C51639874 @default.
• W2954246512 hasConcept C55493867 @default.
• W2954246512 hasConcept C70721500 @default.
• W2954246512 hasConcept C74187038 @default.
• W2954246512 hasConcept C86803240 @default.
• W2954246512 hasConcept C95444343 @default.
• W2954246512 hasConcept C9760119 @default.
• W2954246512 hasConceptScore W2954246512C104317684 @default.

• next