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• W2954334555 abstract "AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 83:161-166 (2019) - DOI: https://doi.org/10.3354/ame01914 NOTE Assessing extracellular enzymatic activities of biofilms on natural and standardized organic substrata immersed in a stream Muriel Joly*, Clarisse Mallet, Joan Artigas Université Clermont Auvergne, CNRS, Laboratoire Microorganismes Génome et Environnement, 63000 Clermont-Ferrand, France *Corresponding author: muriel.joly@uca.fr ABSTRACT: Extracellular enzymatic activities (EEAs) of microbial biofilms are a key component of organic matter transformation in streams, but their measurement, in particular on leaves, is often associated with high variability. We investigated the potential of standardized substrata (cotton strips and wooden [poplar] sticks) to reduce measurement heterogeneity of 3 microbial EEAs (β-D-glucosidase [GLU], N-acetyl-glucosaminidase [NAG] and phosphatase [PHO]) in comparison with leaves. Substrata were placed in a temperate forest stream for 8 wk. Global enzymatic activities were within the same order of magnitude among substrata. Variability among replicates was dependent on the time phase of the experiment: in the short term (≤2 wk), leaves, wood and cotton exhibited the lowest variation among replicates for PHO, GLU and NAG activity measurements, respectively, but over a longer period (>2 wk), cotton was the most suitable substrata to precisely evaluate the 3 activities. Cotton is thus of great interest for monitoring extracellular enzymatic activities in lotic environments. KEY WORDS: Stream · Cotton · Wood · β-glucosidase · N-acetylglucosaminidase · Phosphatase Full text in pdf format PreviousNextCite this article as: Joly M, Mallet C, Artigas J (2019) Assessing extracellular enzymatic activities of biofilms on natural and standardized organic substrata immersed in a stream. Aquat Microb Ecol 83:161-166. https://doi.org/10.3354/ame01914 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 83, No. 2. Online publication date: August 08, 2019 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2019 Inter-Research." @default.
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• W2954334555 title "Assessing extracellular enzymatic activities of biofilms on natural and standardized organic substrata immersed in a stream" @default.
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