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- W2954366991 abstract "T cells play an important role in the immune system where T cell receptors (TCR) identify foreign pathogens bound to major histocompability complex (MHC) molecules on antigen presenting cells (APCs). The detection of an antigen causes the T cell to trigger, which is an activation of the cell that results in a cellular immune response. This triggering process is not fully understood and studying the T cells in vivo is difficult due to the sheer amount of interactions between the hundreds of different proteins on the cell surface. This is circumvented by studying the cells in vitro on a supported lipid bilayer (SLB) which resembles a cell membrane of an opposing cell. In this thesis the interactions between T cells and bilayers of different lipid compositions were studied, where all different surfaces produced triggered cells. Surprisingly, the antibody OKT3 had the lowest number of triggering cells whereas the bilayers with 1%, 2% and 5% of the lipid DGS-NTA produced the highest number of triggering cells independent of the percentage. However, the OKT3 coated surface caused the quickest triggering whereas the other surfaces had slower but similar triggering times. The interaction between cells and membrane proteins are studied by binding membrane proteins to an SLB and adding cells. Next in this thesis, the T cell protein rat CD2 was non-covalently bound to an SLB, where the detachment of the protein was measured over time in different buffer mixtures to observe whether the buffers had an effect on the bound protein. The RPMI media had the highest influence on the detachment of protein. The FBS component of RPMI media stopped rat CD2 from binding to the bilayer but did not influence the detachment of the proteins that successfully bound to the bilayer. In the last experiment the interaction between T cells and rat CD2 bound to an SLB was studied. It was known that the cells would trigger when binding to the CD2 but not how much of the protein is needed for triggering. The protein accumulated under the cell after binding to non-native rat CD48 on the cell surface. The cells were triggered both before and after visible protein accumulations on the bilayer and therefore it could not be concluded whether a certain level of protein accumulation was necessary for the cells to trigger. (Less)" @default.
- W2954366991 created "2019-07-12" @default.
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- W2954366991 date "2019-01-01" @default.
- W2954366991 modified "2023-09-27" @default.
- W2954366991 title "Understanding T-cell triggering under different in vitro conditions" @default.
- W2954366991 hasPublicationYear "2019" @default.
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