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• W2954420967 abstract "Microbial production of fuels and chemicals from renewable and readily available biomass is a sustainable and economically attractive alternative to petroleum-based production. Because of its unusual tolerance to highly acidic conditions, I. orientalis is a promising potential candidate for the manufacture of valued organic acids. Nevertheless, reliable and efficient genetic engineering tools in I. orientalis are limited. The results outlined in this paper describe a stable episomal ARS-containing plasmid and the first CRISPR/Cas9-based system for gene disruptions in I. orientalis , paving the way for applying genome engineering and metabolic engineering strategies and tools in this microorganism for production of fuels and chemicals." @default.
• W2954420967 created "2019-07-12" @default.
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• W2954420967 date "2019-06-26" @default.
• W2954420967 modified "2023-10-16" @default.
• W2954420967 title "Development of a CRISPR/Cas9-Based Tool for Gene Deletion in <i>Issatchenkia orientalis</i>" @default.
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• W2954420967 doi "https://doi.org/10.1128/msphere.00345-19" @default.
• W2954420967 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6595149" @default.
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• W2954420967 hasPublicationYear "2019" @default.
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