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• W2954619346 endingPage "12013" @default.
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• W2954619346 abstract "Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C+ ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C+ Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes." @default.
• W2954619346 created "2019-07-12" @default.
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• W2954619346 date "2019-08-26" @default.
• W2954619346 modified "2023-10-16" @default.
• W2954619346 title "Infrared Spectroscopic Observation of a G–C ⁺ Hoogsteen Base Pair in the DNA:TATA‐Box Binding Protein Complex Under Solution Conditions" @default.
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• W2954619346 doi "https://doi.org/10.1002/anie.201902693" @default.
• W2954619346 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6719543" @default.
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