Matches in SemOpenAlex for { <https://semopenalex.org/work/W2954631326> ?p ?o ?g. }
- W2954631326 abstract "ABSTRACT Unlike C 3 plants, Crassulacean acid metabolism (CAM) plants fix CO 2 in the dark using phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31). PPC combines PEP with CO 2 (as HCO 3 − ), forming oxaloacetate that is rapidly converted to malate, leading to vacuolar malic acid accumulation that peaks phased to dawn. In the light period, malate decarboxylation concentrates CO 2 around RuBisCO for secondary fixation. CAM mutants lacking PPC have not been described. Here, RNAi was employed to silence CAM isogene PPC1 in Kalanchoë laxiflora . Line rPPC1-B lacked PPC1 transcripts, PPC activity, dark period CO 2 fixation, and nocturnal malate accumulation. Light period stomatal closure was also perturbed, and the plants displayed reduced but detectable dark period stomatal conductance, and arrhythmia of the CAM CO 2 fixation circadian rhythm under constant light and temperature (LL) free-running conditions. By contrast, the rhythm of delayed fluorescence was enhanced in plants lacking PPC1 . Furthermore, a subset of gene transcripts within the central circadian oscillator were up-regulated and oscillated robustly. The regulation guard cell genes involved controlling stomatal movements was also altered in rPPC1-B . This provided direct evidence that altered regulatory patterns of key guard cell signaling genes are linked with the characteristic inverse pattern of stomatal opening and closing during CAM." @default.
- W2954631326 created "2019-07-12" @default.
- W2954631326 creator A5003224959 @default.
- W2954631326 creator A5047686097 @default.
- W2954631326 creator A5058762072 @default.
- W2954631326 creator A5062764928 @default.
- W2954631326 creator A5075718407 @default.
- W2954631326 creator A5078809383 @default.
- W2954631326 creator A5084605117 @default.
- W2954631326 date "2019-06-27" @default.
- W2954631326 modified "2023-09-27" @default.
- W2954631326 title "Silencing<i>PHOSPHOENOLPYRUVATE CARBOXYLASE1</i>in the Obligate Crassulacean Acid Metabolism Species<i>Kalanchoë laxiflora</i>causes Reversion to C<sub>3</sub>-like Metabolism and Amplifies Rhythmicity in a Subset of Core Circadian Clock Genes" @default.
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