Matches in SemOpenAlex for { <https://semopenalex.org/work/W2954632344> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2954632344 endingPage "253" @default.
- W2954632344 startingPage "247" @default.
- W2954632344 abstract "Abstract Background The interest for vitamin D has exponentially increased testing demand for 25-hydroxy vitamin D [25(OH)D]. Consequently, many laboratories are switching from LC-MS/MS methods to automated, high-throughput immunoassays. One of the major potential issues with these assays has been the lack of cross-reactivity with 25(OH)D2. Methods We have evaluated the Roche Elecsys vitamin D total II assay for accuracy by comparing 79 patient samples with LC-MS/MS. The cross-reactivity for 25(OH)D2 was evaluated by analyzing samples with high 25(OH)D2 separately and estimating 25(OH)D2 recovery, as well as by spiking of 25(OH)D2. The assay was further evaluated for precision, linearity, sample type, and common interferences. Results There was mostly good agreement between the Elecsys and LC-MS/MS assays (Deming regression: y = 0.95x + 0.70), with an overall bias of 2.3% (−0.84 ng/mL). However, there were 6 out of 79 (7.6%) discordant samples. The Deming regression for samples with high 25(OH)D2 compared to LC-MS/MS showed similar slope and intercept (y = 0.97x − 1.1). The average recovery of 25(OH)D2 for these samples was 90%. The initial precision studies were in general agreement with the package insert, but long-term clinical use showed higher-than-claimed imprecision (11.7%–14.4% at 12 ng/mL and 6.9%–7.6% at 27 ng/mL; claimed: 7.2% and 5.0%, respectively). We observed 1 falsely high result in plasma, an issue previously addressed by Roche in a medical device correction. Conclusions The analytical performance of the Roche Vitamin D assay was acceptable, and the assay had a good cross-reactivity for 25(OH)D2." @default.
- W2954632344 created "2019-07-12" @default.
- W2954632344 creator A5016171992 @default.
- W2954632344 creator A5023528057 @default.
- W2954632344 creator A5032209506 @default.
- W2954632344 creator A5075934531 @default.
- W2954632344 creator A5091328328 @default.
- W2954632344 date "2019-09-01" @default.
- W2954632344 modified "2023-10-15" @default.
- W2954632344 title "Evaluation of a New Generation Automated Assay for 25-Hydroxy Vitamin D Based on Competitive Protein Binding" @default.
- W2954632344 cites W1464788707 @default.
- W2954632344 cites W1990777688 @default.
- W2954632344 cites W2028458952 @default.
- W2954632344 cites W2055480626 @default.
- W2954632344 cites W2096239379 @default.
- W2954632344 cites W2127609720 @default.
- W2954632344 cites W2153059487 @default.
- W2954632344 cites W2165526847 @default.
- W2954632344 cites W2320922018 @default.
- W2954632344 cites W2751992283 @default.
- W2954632344 cites W2766182190 @default.
- W2954632344 cites W2803443381 @default.
- W2954632344 cites W2808368070 @default.
- W2954632344 cites W2884210702 @default.
- W2954632344 cites W975328870 @default.
- W2954632344 doi "https://doi.org/10.1373/jalm.2018.028415" @default.
- W2954632344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31639671" @default.
- W2954632344 hasPublicationYear "2019" @default.
- W2954632344 type Work @default.
- W2954632344 sameAs 2954632344 @default.
- W2954632344 citedByCount "5" @default.
- W2954632344 countsByYear W29546323442020 @default.
- W2954632344 countsByYear W29546323442021 @default.
- W2954632344 countsByYear W29546323442022 @default.
- W2954632344 crossrefType "journal-article" @default.
- W2954632344 hasAuthorship W2954632344A5016171992 @default.
- W2954632344 hasAuthorship W2954632344A5023528057 @default.
- W2954632344 hasAuthorship W2954632344A5032209506 @default.
- W2954632344 hasAuthorship W2954632344A5075934531 @default.
- W2954632344 hasAuthorship W2954632344A5091328328 @default.
- W2954632344 hasBestOaLocation W29546323441 @default.
- W2954632344 hasConcept C105795698 @default.
- W2954632344 hasConcept C126322002 @default.
- W2954632344 hasConcept C159654299 @default.
- W2954632344 hasConcept C185592680 @default.
- W2954632344 hasConcept C203014093 @default.
- W2954632344 hasConcept C2776940978 @default.
- W2954632344 hasConcept C2780420688 @default.
- W2954632344 hasConcept C2909597000 @default.
- W2954632344 hasConcept C33923547 @default.
- W2954632344 hasConcept C43617362 @default.
- W2954632344 hasConcept C48921125 @default.
- W2954632344 hasConcept C55493867 @default.
- W2954632344 hasConcept C71924100 @default.
- W2954632344 hasConceptScore W2954632344C105795698 @default.
- W2954632344 hasConceptScore W2954632344C126322002 @default.
- W2954632344 hasConceptScore W2954632344C159654299 @default.
- W2954632344 hasConceptScore W2954632344C185592680 @default.
- W2954632344 hasConceptScore W2954632344C203014093 @default.
- W2954632344 hasConceptScore W2954632344C2776940978 @default.
- W2954632344 hasConceptScore W2954632344C2780420688 @default.
- W2954632344 hasConceptScore W2954632344C2909597000 @default.
- W2954632344 hasConceptScore W2954632344C33923547 @default.
- W2954632344 hasConceptScore W2954632344C43617362 @default.
- W2954632344 hasConceptScore W2954632344C48921125 @default.
- W2954632344 hasConceptScore W2954632344C55493867 @default.
- W2954632344 hasConceptScore W2954632344C71924100 @default.
- W2954632344 hasIssue "2" @default.
- W2954632344 hasLocation W29546323441 @default.
- W2954632344 hasLocation W29546323442 @default.
- W2954632344 hasOpenAccess W2954632344 @default.
- W2954632344 hasPrimaryLocation W29546323441 @default.
- W2954632344 hasRelatedWork W2006194096 @default.
- W2954632344 hasRelatedWork W2008373365 @default.
- W2954632344 hasRelatedWork W2026718706 @default.
- W2954632344 hasRelatedWork W2057676963 @default.
- W2954632344 hasRelatedWork W2079372671 @default.
- W2954632344 hasRelatedWork W2084401751 @default.
- W2954632344 hasRelatedWork W2086078716 @default.
- W2954632344 hasRelatedWork W2197250283 @default.
- W2954632344 hasRelatedWork W2415923358 @default.
- W2954632344 hasRelatedWork W4298857202 @default.
- W2954632344 hasVolume "4" @default.
- W2954632344 isParatext "false" @default.
- W2954632344 isRetracted "false" @default.
- W2954632344 magId "2954632344" @default.
- W2954632344 workType "article" @default.