Matches in SemOpenAlex for { <https://semopenalex.org/work/W2954734963> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2954734963 abstract "Clinical applications of precision oncology require accurate tests that can distinguish tumor-specific mutations from errors introduced at each step of next generation sequencing (NGS). For NGS to successfully improve patient lives, discriminating between true mutations and artifacts is crucial. We systematically interrogated somatic mutations in paired tumor normal cell lines to identify factors affecting detection reproducibility and accuracy. Different types of samples with varying input amount and tumor purity were processed using multiple library construction protocols. Whole-genome and whole-exome sequencing were carried out at six sequencing centers followed by processing with nine bioinformatics pipelines to evaluate their reproducibility. We identified artifacts due to sample and library processing and evaluated the capabilities and limitations of bioinformatics tools for artifact detection and removal. By examining the interaction and effect of various wet lab and computational parameters concomitantly, here we recommend actionable best practices for mutation detection in clinical applications using NGS technologies. Citation Format: Wenming Xiao, The Somatic Mutation Working Group of the SEQC-IIConsortium. Achieving reproducibility and accuracy in cancer mutation detection with whole-genome and whole-exome sequencing [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 3525." @default.
- W2954734963 created "2019-07-12" @default.
- W2954734963 creator A5079978766 @default.
- W2954734963 date "2019-07-01" @default.
- W2954734963 modified "2023-09-27" @default.
- W2954734963 title "Abstract 3525: Achieving reproducibility and accuracy in cancer mutation detection with whole-genome and whole-exome sequencing" @default.
- W2954734963 doi "https://doi.org/10.1158/1538-7445.sabcs18-3525" @default.
- W2954734963 hasPublicationYear "2019" @default.
- W2954734963 type Work @default.
- W2954734963 sameAs 2954734963 @default.
- W2954734963 citedByCount "0" @default.
- W2954734963 crossrefType "proceedings-article" @default.
- W2954734963 hasAuthorship W2954734963A5079978766 @default.
- W2954734963 hasConcept C104317684 @default.
- W2954734963 hasConcept C105795698 @default.
- W2954734963 hasConcept C10590036 @default.
- W2954734963 hasConcept C121608353 @default.
- W2954734963 hasConcept C126322002 @default.
- W2954734963 hasConcept C141231307 @default.
- W2954734963 hasConcept C16671776 @default.
- W2954734963 hasConcept C24432333 @default.
- W2954734963 hasConcept C33923547 @default.
- W2954734963 hasConcept C501734568 @default.
- W2954734963 hasConcept C51679486 @default.
- W2954734963 hasConcept C54355233 @default.
- W2954734963 hasConcept C60644358 @default.
- W2954734963 hasConcept C70721500 @default.
- W2954734963 hasConcept C71924100 @default.
- W2954734963 hasConcept C86803240 @default.
- W2954734963 hasConcept C9893847 @default.
- W2954734963 hasConceptScore W2954734963C104317684 @default.
- W2954734963 hasConceptScore W2954734963C105795698 @default.
- W2954734963 hasConceptScore W2954734963C10590036 @default.
- W2954734963 hasConceptScore W2954734963C121608353 @default.
- W2954734963 hasConceptScore W2954734963C126322002 @default.
- W2954734963 hasConceptScore W2954734963C141231307 @default.
- W2954734963 hasConceptScore W2954734963C16671776 @default.
- W2954734963 hasConceptScore W2954734963C24432333 @default.
- W2954734963 hasConceptScore W2954734963C33923547 @default.
- W2954734963 hasConceptScore W2954734963C501734568 @default.
- W2954734963 hasConceptScore W2954734963C51679486 @default.
- W2954734963 hasConceptScore W2954734963C54355233 @default.
- W2954734963 hasConceptScore W2954734963C60644358 @default.
- W2954734963 hasConceptScore W2954734963C70721500 @default.
- W2954734963 hasConceptScore W2954734963C71924100 @default.
- W2954734963 hasConceptScore W2954734963C86803240 @default.
- W2954734963 hasConceptScore W2954734963C9893847 @default.
- W2954734963 hasLocation W29547349631 @default.
- W2954734963 hasOpenAccess W2954734963 @default.
- W2954734963 hasPrimaryLocation W29547349631 @default.
- W2954734963 hasRelatedWork W1524235283 @default.
- W2954734963 hasRelatedWork W2277089966 @default.
- W2954734963 hasRelatedWork W2285921773 @default.
- W2954734963 hasRelatedWork W2340271117 @default.
- W2954734963 hasRelatedWork W2344121078 @default.
- W2954734963 hasRelatedWork W2482598544 @default.
- W2954734963 hasRelatedWork W2565978311 @default.
- W2954734963 hasRelatedWork W2605525619 @default.
- W2954734963 hasRelatedWork W2739858610 @default.
- W2954734963 hasRelatedWork W2741073307 @default.
- W2954734963 hasRelatedWork W2887434772 @default.
- W2954734963 hasRelatedWork W2887438700 @default.
- W2954734963 hasRelatedWork W2954629631 @default.
- W2954734963 hasRelatedWork W2954846908 @default.
- W2954734963 hasRelatedWork W3011714190 @default.
- W2954734963 hasRelatedWork W3082993973 @default.
- W2954734963 hasRelatedWork W3135295354 @default.
- W2954734963 hasRelatedWork W3143203499 @default.
- W2954734963 hasRelatedWork W3194965953 @default.
- W2954734963 hasRelatedWork W3196953684 @default.
- W2954734963 isParatext "false" @default.
- W2954734963 isRetracted "false" @default.
- W2954734963 magId "2954734963" @default.
- W2954734963 workType "article" @default.