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- W2954790590 abstract "The bovine cumulus-oocyte complex (COC) is capable of converting cortisone, an inert glucocorticoid to active cortisol. This mechanism is mediated by 11β-hydroxysteroid oxidoreductase type 1 (HSD11B1), whose expression dramatically increases in the mature COC. In this study, we investigate the time course expression of HSD11B1 and the enzyme activity in the bovine COC undergoing maturation and fertilization in relation to key events taking place in the COC. Bovine COCs were subjected to in vitro maturation (IVM) and fertilization (IVF). The activities of HSD11B1 and HSD11B2, which mediates the opposite reaction, were measured using a radiometric conversion assay. In parallel studies, cumulus expansion, P4 production and the expression of genes associated with ovulation were measured. The reductive activity of HSD11B1 increased in the latter half of IVM and remained high during IVF, whereas the oxidative activity of HSD11B2 remained unchanged over both periods. Consequently, the net glucocorticoid metabolism in the bovine COC shifted from inactivation to activation around the time of ovulation and fertilization. The increase in HSD11B1 expression lagged behind that of P4 increase and cumulus expansion but ahead of the expressions of genes responsible for PGE2 synthesis. The reductive activity of HSD11B1 was well correlated with the cumulus expansion rate. This outcome indicates that the ability of the cumulus to activate glucocorticoids is related to its ability to synthesize hyaluronan. These results also indicate that the activation of HSD11B1 is an integral part of the sequential events taking place at the ovulation and fertilization in the bovine COC." @default.
- W2954790590 created "2019-07-12" @default.
- W2954790590 creator A5052750469 @default.
- W2954790590 creator A5084078519 @default.
- W2954790590 date "2019-07-01" @default.
- W2954790590 modified "2023-09-27" @default.
- W2954790590 title "Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF" @default.
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- W2954790590 doi "https://doi.org/10.1530/ec-19-0188" @default.
- W2954790590 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6652248" @default.
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- W2954790590 hasPublicationYear "2019" @default.
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