Matches in SemOpenAlex for { <https://semopenalex.org/work/W2954926818> ?p ?o ?g. }
- W2954926818 abstract "Outer membrane proteins (OMPs) in Gram-negative bacteria dictate permeability of metabolites, antibiotics, and toxins. Elucidating the structure-function relationships governing OMPs within native membrane environments remains challenging. We constructed a diverse library of >3000 monoclonal antibodies to assess the roles of extracellular loops (ECLs) in LptD, an essential OMP that inserts lipopolysaccharide into the outer membrane of Escherichia coli. Epitope binning and mapping experiments with LptD-loop-deletion mutants demonstrated that 7 of the 13 ECLs are targeted by antibodies. Only ECLs inaccessible to antibodies were required for the structure or function of LptD. Our results suggest that antibody-accessible loops evolved to protect key extracellular regions of LptD, but are themselves dispensable. Supporting this hypothesis, no α-LptD antibody interfered with essential functions of LptD. Our experimental workflow enables structure-function studies of OMPs in native cellular environments, provides unexpected insight into LptD, and presents a method to assess the therapeutic potential of antibody targeting." @default.
- W2954926818 created "2019-07-12" @default.
- W2954926818 creator A5001385114 @default.
- W2954926818 creator A5011088509 @default.
- W2954926818 creator A5013120773 @default.
- W2954926818 creator A5018702345 @default.
- W2954926818 creator A5024034539 @default.
- W2954926818 creator A5029608268 @default.
- W2954926818 creator A5039127243 @default.
- W2954926818 creator A5043416054 @default.
- W2954926818 creator A5046085402 @default.
- W2954926818 creator A5046256348 @default.
- W2954926818 creator A5048565515 @default.
- W2954926818 creator A5054080482 @default.
- W2954926818 creator A5058739493 @default.
- W2954926818 creator A5066331952 @default.
- W2954926818 creator A5066701038 @default.
- W2954926818 creator A5067614500 @default.
- W2954926818 creator A5068583888 @default.
- W2954926818 creator A5077068463 @default.
- W2954926818 creator A5077675694 @default.
- W2954926818 creator A5084815841 @default.
- W2954926818 creator A5085512866 @default.
- W2954926818 creator A5088493467 @default.
- W2954926818 date "2019-06-25" @default.
- W2954926818 modified "2023-10-17" @default.
- W2954926818 title "Massive antibody discovery used to probe structure-function relationships of the essential outer membrane protein LptD." @default.
- W2954926818 cites W1477452881 @default.
- W2954926818 cites W1493374012 @default.
- W2954926818 cites W1531521794 @default.
- W2954926818 cites W1895465241 @default.
- W2954926818 cites W1967725603 @default.
- W2954926818 cites W1969689981 @default.
- W2954926818 cites W1971803523 @default.
- W2954926818 cites W1972858806 @default.
- W2954926818 cites W1972963835 @default.
- W2954926818 cites W1980427104 @default.
- W2954926818 cites W1989699984 @default.
- W2954926818 cites W1994121837 @default.
- W2954926818 cites W2004916218 @default.
- W2954926818 cites W2006365556 @default.
- W2954926818 cites W2020580205 @default.
- W2954926818 cites W2033042708 @default.
- W2954926818 cites W2034401306 @default.
- W2954926818 cites W2058645085 @default.
- W2954926818 cites W2070978351 @default.
- W2954926818 cites W2074867344 @default.
- W2954926818 cites W2079228339 @default.
- W2954926818 cites W2085932417 @default.
- W2954926818 cites W2094749886 @default.
- W2954926818 cites W2096665650 @default.
- W2954926818 cites W2100548922 @default.
- W2954926818 cites W2102528366 @default.
- W2954926818 cites W2106680629 @default.
- W2954926818 cites W2108871577 @default.
- W2954926818 cites W2111967267 @default.
- W2954926818 cites W2116137883 @default.
- W2954926818 cites W2119587675 @default.
- W2954926818 cites W2120061656 @default.
- W2954926818 cites W2129123584 @default.
- W2954926818 cites W2134141380 @default.
- W2954926818 cites W2150014697 @default.
- W2954926818 cites W2162089935 @default.
- W2954926818 cites W2165134866 @default.
- W2954926818 cites W2283351635 @default.
- W2954926818 cites W2286244333 @default.
- W2954926818 cites W2314285321 @default.
- W2954926818 cites W2346561353 @default.
- W2954926818 cites W2560253264 @default.
- W2954926818 cites W2568765466 @default.
- W2954926818 cites W2598833252 @default.
- W2954926818 cites W2734573011 @default.
- W2954926818 cites W2753791808 @default.
- W2954926818 cites W2793159453 @default.
- W2954926818 cites W2802039831 @default.
- W2954926818 cites W2806396616 @default.
- W2954926818 cites W2883390882 @default.
- W2954926818 cites W2897895147 @default.
- W2954926818 cites W2901735769 @default.
- W2954926818 cites W2952135714 @default.
- W2954926818 doi "https://doi.org/10.7554/elife.46258" @default.
- W2954926818 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6592684" @default.
- W2954926818 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31237236" @default.
- W2954926818 hasPublicationYear "2019" @default.
- W2954926818 type Work @default.
- W2954926818 sameAs 2954926818 @default.
- W2954926818 citedByCount "22" @default.
- W2954926818 countsByYear W29549268182019 @default.
- W2954926818 countsByYear W29549268182020 @default.
- W2954926818 countsByYear W29549268182021 @default.
- W2954926818 countsByYear W29549268182022 @default.
- W2954926818 countsByYear W29549268182023 @default.
- W2954926818 crossrefType "journal-article" @default.
- W2954926818 hasAuthorship W2954926818A5001385114 @default.
- W2954926818 hasAuthorship W2954926818A5011088509 @default.
- W2954926818 hasAuthorship W2954926818A5013120773 @default.
- W2954926818 hasAuthorship W2954926818A5018702345 @default.
- W2954926818 hasAuthorship W2954926818A5024034539 @default.
- W2954926818 hasAuthorship W2954926818A5029608268 @default.
- W2954926818 hasAuthorship W2954926818A5039127243 @default.