FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955126051> ?p ?o ?g. }

• W2955126051 endingPage "478" @default.
• W2955126051 startingPage "467" @default.
• W2955126051 abstract "An enzyme-coupled colorimetric assay for quantification of urinary sarcosine was developed. The proposed method is a specific reaction based on hydrogen peroxide (H2O2) formation via sarcosine oxidase (SOX). The liberated H2O2 reacts with Amplex Red in the presence of horseradish peroxidase (HRP) to produce the red-fluorescent oxidation product, resorufin, which can be measured spectrophotometrically (OD570). The method was performed in the 96-well microtiter plate. Reaction conditions, such as pH and reaction time were optimized. At the optimum conditions, the limit of detection (LOD) and quantification (LOQ) were found to be 0.7 and 1 µM, respectively. A good linearity was revealed with a coefficient of 0.990. The assay showed no significant interference from ascorbic acid, glucose and bilirubin. In addition, it is extremely specific for sarcosine rather than other amino acids. The determination of sarcosine in human urine displayed high accuracy and good reproducibility. This method is promising to differentiate prostate cancer patients from healthy subjects according to urinary sarcosine level. Altogether, this study provides a rapid, simple and specific tool to determine urinary sarcosine which could be useful for prostate cancer diagnosis." @default.
• W2955126051 created "2019-07-12" @default.
• W2955126051 creator A5012302046 @default.
• W2955126051 creator A5016654817 @default.
• W2955126051 creator A5039485228 @default.
• W2955126051 creator A5041946423 @default.
• W2955126051 creator A5046083602 @default.
• W2955126051 creator A5056315115 @default.
• W2955126051 creator A5077906262 @default.
• W2955126051 date "2018-01-01" @default.
• W2955126051 modified "2023-10-01" @default.
• W2955126051 title "Development of sarcosine quantification in urine based on enzyme-coupled colorimetric method for prostate cancer diagnosis." @default.
• W2955126051 cites W1536654439 @default.
• W2955126051 cites W1667881669 @default.
• W2955126051 cites W1925569049 @default.
• W2955126051 cites W1972716867 @default.
• W2955126051 cites W1978882112 @default.
• W2955126051 cites W1984121561 @default.
• W2955126051 cites W1984740654 @default.
• W2955126051 cites W2000043291 @default.
• W2955126051 cites W2011707447 @default.
• W2955126051 cites W2013557200 @default.
• W2955126051 cites W2021278678 @default.
• W2955126051 cites W2023517354 @default.
• W2955126051 cites W2027554102 @default.
• W2955126051 cites W2040143283 @default.
• W2955126051 cites W2045706647 @default.
• W2955126051 cites W2052884790 @default.
• W2955126051 cites W2055612753 @default.
• W2955126051 cites W2056476612 @default.
• W2955126051 cites W2064212123 @default.
• W2955126051 cites W2067009296 @default.
• W2955126051 cites W2067535546 @default.
• W2955126051 cites W2084860101 @default.
• W2955126051 cites W2094223469 @default.
• W2955126051 cites W2098354688 @default.
• W2955126051 cites W2128365375 @default.
• W2955126051 cites W2130105421 @default.
• W2955126051 cites W2138495794 @default.
• W2955126051 cites W2149673768 @default.
• W2955126051 cites W2155680682 @default.
• W2955126051 cites W2286163737 @default.
• W2955126051 cites W2331863668 @default.
• W2955126051 cites W2335082892 @default.
• W2955126051 cites W2409674124 @default.
• W2955126051 cites W2519987324 @default.
• W2955126051 cites W2594484552 @default.
• W2955126051 cites W2883994748 @default.
• W2955126051 doi "https://doi.org/10.17179/excli2018-145" @default.
• W2955126051 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6046622" @default.
• W2955126051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30034310" @default.
• W2955126051 hasPublicationYear "2018" @default.
• W2955126051 type Work @default.
• W2955126051 sameAs 2955126051 @default.
• W2955126051 citedByCount "5" @default.
• W2955126051 countsByYear W29551260512018 @default.
• W2955126051 countsByYear W29551260512020 @default.
• W2955126051 countsByYear W29551260512022 @default.
• W2955126051 crossrefType "journal-article" @default.
• W2955126051 hasAuthorship W2955126051A5012302046 @default.
• W2955126051 hasAuthorship W2955126051A5016654817 @default.
• W2955126051 hasAuthorship W2955126051A5039485228 @default.
• W2955126051 hasAuthorship W2955126051A5041946423 @default.
• W2955126051 hasAuthorship W2955126051A5046083602 @default.
• W2955126051 hasAuthorship W2955126051A5056315115 @default.
• W2955126051 hasAuthorship W2955126051A5077906262 @default.
• W2955126051 hasConcept C119128265 @default.
• W2955126051 hasConcept C126322002 @default.
• W2955126051 hasConcept C181199279 @default.
• W2955126051 hasConcept C185592680 @default.
• W2955126051 hasConcept C2777676563 @default.
• W2955126051 hasConcept C2777756961 @default.
• W2955126051 hasConcept C2779943223 @default.
• W2955126051 hasConcept C2780026642 @default.
• W2955126051 hasConcept C43617362 @default.
• W2955126051 hasConcept C515207424 @default.
• W2955126051 hasConcept C533411734 @default.
• W2955126051 hasConcept C55493867 @default.
• W2955126051 hasConcept C64299311 @default.
• W2955126051 hasConcept C71924100 @default.
• W2955126051 hasConcept C77411442 @default.
• W2955126051 hasConceptScore W2955126051C119128265 @default.
• W2955126051 hasConceptScore W2955126051C126322002 @default.
• W2955126051 hasConceptScore W2955126051C181199279 @default.
• W2955126051 hasConceptScore W2955126051C185592680 @default.
• W2955126051 hasConceptScore W2955126051C2777676563 @default.
• W2955126051 hasConceptScore W2955126051C2777756961 @default.
• W2955126051 hasConceptScore W2955126051C2779943223 @default.
• W2955126051 hasConceptScore W2955126051C2780026642 @default.
• W2955126051 hasConceptScore W2955126051C43617362 @default.
• W2955126051 hasConceptScore W2955126051C515207424 @default.
• W2955126051 hasConceptScore W2955126051C533411734 @default.
• W2955126051 hasConceptScore W2955126051C55493867 @default.
• W2955126051 hasConceptScore W2955126051C64299311 @default.
• W2955126051 hasConceptScore W2955126051C71924100 @default.
• W2955126051 hasConceptScore W2955126051C77411442 @default.
• W2955126051 hasLocation W29551260511 @default.
• W2955126051 hasOpenAccess W2955126051 @default.

• next