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• W2955180713 abstract "Alkaline phosphatase (ALP) is extensively used as a clinical biomarker because of its close relevance with a variety of diseases. Thus, exploring reliable and practical methods for ALP analysis is of great significance. In the present work, we explored MIL-53(Fe) as a bifunctional platform with pyrophosphate (PPi)-mediated peroxidase-like activity and oxidation-stimulated fluorescence switching for ALP sensing. The proposed MIL-53(Fe) could exhibit favorable peroxidase-mimicking activity to catalytically decompose H2O2 to hydroxyl radicals, which had strong oxidizing ability to oxidize the terephthalic acid bridging ligand, resulting in the oxidation-stimulated turn-on fluorescence of MIL-53(Fe) itself. Due to the strong coordination interaction between PPi and Fe3+, the former with a relatively large molecular structure was able to inhibit the catalytic activity of MIL-53(Fe) via capping active Fe3+ sites, leading to the suppression of its self-fluorescence response. When ALP was present, it could hydrolyze the PPi inhibitor and restore the dual functions of MIL-53(Fe) to provide fluorescence again. With the above principle, highly sensitive and selective determination of ALP with a linear scope of 2-80 U L-1 and a detection limit down to 0.7 U L-1 was achieved. The MIL-53(Fe) was also demonstrated to be very reliable in measuring the target in human serum, indicating its great promise as an integrated tool for ALP detection in clinical practice." @default.
• W2955180713 created "2019-07-12" @default.
• W2955180713 creator A5022526821 @default.
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• W2955180713 creator A5074021241 @default.
• W2955180713 date "2019-01-01" @default.
• W2955180713 modified "2023-10-14" @default.
• W2955180713 title "Bifunctional MIL-53(Fe) with pyrophosphate-mediated peroxidase-like activity and oxidation-stimulated fluorescence switching for alkaline phosphatase detection" @default.
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• W2955180713 doi "https://doi.org/10.1039/c9tb00951e" @default.
• W2955180713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31389965" @default.
• W2955180713 hasPublicationYear "2019" @default.
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