FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955234098> ?p ?o ?g. }

• W2955234098 endingPage "68" @default.
• W2955234098 startingPage "57" @default.
• W2955234098 abstract "Abstract Ribosome profiling (Ribo‐seq) can potentially provide detailed information about ribosome position on transcripts and estimates of protein translation levels in vivo . Hsp90 chaperones, which play a critical role in stress tolerance, have characteristic patterns of differential expression under nonstressed and heat shock conditions. By analyzing published Ribo‐seq data for the Hsp90 chaperones in S. cerevisiae , we find wide‐ranging artifacts originating from “multimapping” reads (reads that cannot be uniquely assigned to one position), which constitute ~25% of typical S. cerevisiae Ribo‐seq datasets and ~80% of the reads from HEK293 cells. Estimates of Hsp90 protein production as determined by Ribo‐seq are reproducible but not robust, with inferred expression levels that can change 10‐fold depending on how multimapping reads are processed. The differential expression of Hsp90 chaperones under nonstressed and heat shock conditions creates artificial peaks and valleys in their ribosome profiles that give a false impression of regulated translational pausing. Indeed, we find that multimapping can even create an appearance of reproducibility to the shape of the Hsp90 ribosome profiles from biological replicates. Adding further complexity, this artificial reproducibility is dependent on the computational method used to construct the ribosome profile. Given the ubiquity of multimapping reads in Ribo‐seq experiments and the complexity of artifacts associated with multimapping, we developed a publicly available computational tool to identify transcripts most at risk for multimapping artifacts. In doing so, we identify biological pathways that are enriched in multimapping transcripts, meaning that particular biological pathways will be highly susceptible to multimapping artifacts." @default.
• W2955234098 created "2019-07-12" @default.
• W2955234098 creator A5008983289 @default.
• W2955234098 creator A5047011249 @default.
• W2955234098 creator A5090261047 @default.
• W2955234098 date "2019-07-19" @default.
• W2955234098 modified "2023-10-16" @default.
• W2955234098 title "Multimapping confounds ribosome profiling analysis: A case‐study of the Hsp90 molecular chaperone" @default.
• W2955234098 cites W1641544182 @default.
• W2955234098 cites W1758870984 @default.
• W2955234098 cites W1898864635 @default.
• W2955234098 cites W1956379070 @default.
• W2955234098 cites W1964330084 @default.
• W2955234098 cites W1974394989 @default.
• W2955234098 cites W1978509346 @default.
• W2955234098 cites W1984329479 @default.
• W2955234098 cites W2036897871 @default.
• W2955234098 cites W2060421156 @default.
• W2955234098 cites W2071849521 @default.
• W2955234098 cites W2079517684 @default.
• W2955234098 cites W2081933515 @default.
• W2955234098 cites W2086157203 @default.
• W2955234098 cites W2086471971 @default.
• W2955234098 cites W2099879295 @default.
• W2955234098 cites W2102221598 @default.
• W2955234098 cites W2102619694 @default.
• W2955234098 cites W2103017472 @default.
• W2955234098 cites W2108234281 @default.
• W2955234098 cites W2109602972 @default.
• W2955234098 cites W2116041602 @default.
• W2955234098 cites W2117131162 @default.
• W2955234098 cites W2124985265 @default.
• W2955234098 cites W2126096918 @default.
• W2955234098 cites W2147411714 @default.
• W2955234098 cites W2147555013 @default.
• W2955234098 cites W2147837708 @default.
• W2955234098 cites W2159675211 @default.
• W2955234098 cites W2160215304 @default.
• W2955234098 cites W2161616648 @default.
• W2955234098 cites W2162170988 @default.
• W2955234098 cites W2163672244 @default.
• W2955234098 cites W2167725902 @default.
• W2955234098 cites W2262417550 @default.
• W2955234098 cites W2266589141 @default.
• W2955234098 cites W2290865014 @default.
• W2955234098 cites W2346311008 @default.
• W2955234098 cites W2549536905 @default.
• W2955234098 cites W2559466477 @default.
• W2955234098 cites W2588482805 @default.
• W2955234098 cites W2604808360 @default.
• W2955234098 cites W2749718530 @default.
• W2955234098 cites W2756085100 @default.
• W2955234098 cites W2766459919 @default.
• W2955234098 cites W2768325741 @default.
• W2955234098 cites W2904663585 @default.
• W2955234098 cites W2950257784 @default.
• W2955234098 cites W2951358362 @default.
• W2955234098 cites W4250359879 @default.
• W2955234098 doi "https://doi.org/10.1002/prot.25766" @default.
• W2955234098 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6901744" @default.
• W2955234098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31254414" @default.
• W2955234098 hasPublicationYear "2019" @default.
• W2955234098 type Work @default.
• W2955234098 sameAs 2955234098 @default.
• W2955234098 citedByCount "4" @default.
• W2955234098 countsByYear W29552340982019 @default.
• W2955234098 countsByYear W29552340982020 @default.
• W2955234098 countsByYear W29552340982021 @default.
• W2955234098 crossrefType "journal-article" @default.
• W2955234098 hasAuthorship W2955234098A5008983289 @default.
• W2955234098 hasAuthorship W2955234098A5047011249 @default.
• W2955234098 hasAuthorship W2955234098A5090261047 @default.
• W2955234098 hasBestOaLocation W29552340982 @default.
• W2955234098 hasConcept C104317684 @default.
• W2955234098 hasConcept C105580179 @default.
• W2955234098 hasConcept C149364088 @default.
• W2955234098 hasConcept C182325514 @default.
• W2955234098 hasConcept C205260736 @default.
• W2955234098 hasConcept C2775932338 @default.
• W2955234098 hasConcept C41008148 @default.
• W2955234098 hasConcept C54355233 @default.
• W2955234098 hasConcept C60644358 @default.
• W2955234098 hasConcept C67705224 @default.
• W2955234098 hasConcept C70721500 @default.
• W2955234098 hasConcept C86803240 @default.
• W2955234098 hasConcept C88478588 @default.
• W2955234098 hasConceptScore W2955234098C104317684 @default.
• W2955234098 hasConceptScore W2955234098C105580179 @default.
• W2955234098 hasConceptScore W2955234098C149364088 @default.
• W2955234098 hasConceptScore W2955234098C182325514 @default.
• W2955234098 hasConceptScore W2955234098C205260736 @default.
• W2955234098 hasConceptScore W2955234098C2775932338 @default.
• W2955234098 hasConceptScore W2955234098C41008148 @default.
• W2955234098 hasConceptScore W2955234098C54355233 @default.
• W2955234098 hasConceptScore W2955234098C60644358 @default.
• W2955234098 hasConceptScore W2955234098C67705224 @default.
• W2955234098 hasConceptScore W2955234098C70721500 @default.
• W2955234098 hasConceptScore W2955234098C86803240 @default.

• next