Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955246896> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2955246896 abstract "Optofluidic single-cell genome amplification was used to obtain genome sequences from sub-micron cells collected from the euphotic and mesopelagic zones of the northwestern Sargasso Sea. Plankton cells were visually selected and manually sorted with an optical trap, yielding 20 partial genome sequences representing seven bacterial phyla. Two organisms, E01-9C-26 (Gammaproteobacteria), represented by four single cell genomes, and Opi.OSU.00C, an uncharacterized Verrucomicrobia, were the first of their types retrieved by single cell genome sequencing and were studied in detail. Metagenomic data showed that E01-9C-26 is found throughout the dark ocean, while Opi.OSU.00C was observed to bloom transiently in the nutrient-depleted euphotic zone of the late spring and early summer. The E01-9C-26 genomes had an estimated size of 4.76-5.05 Mbps, and contained O and W-type monooxygenase genes related to methane and ammonium monooxygenases that were previously reported from ocean metagenomes. Metabolic reconstruction indicated E01-9C-26 are likely versatile methylotrophs capable of scavenging C1 compounds, methylated compounds, reduced sulfur compounds, and a wide range of amines, including D-amino acids. The genome sequences identified E01-9C-26 as a source of O and W-type monooxygenase genes related to methane and ammonium monooxygenases that were previously reported from ocean metagenomes, but are of unknown function. In contrast, Opi.OSU.00C genomes encode genes for catabolizing carbohydrate compounds normally associated with eukaryotic phytoplankton. This exploration of optofluidics showed that it was effective for retrieving diverse single-cell bacterioplankton genomes and has potential advantages in microbiology applications that require working with small sample volumes or targeting cells by their morphology." @default.
- W2955246896 created "2019-07-12" @default.
- W2955246896 creator A5022298420 @default.
- W2955246896 creator A5024611841 @default.
- W2955246896 creator A5044471451 @default.
- W2955246896 creator A5052138076 @default.
- W2955246896 creator A5059724032 @default.
- W2955246896 creator A5067084483 @default.
- W2955246896 creator A5076647536 @default.
- W2955246896 creator A5091052718 @default.
- W2955246896 date "2018-06-01" @default.
- W2955246896 modified "2023-09-28" @default.
- W2955246896 title "Optofluidic Single-Cell Genome Amplification of Sub-micron Bacteria in the Ocean Subsurface" @default.
- W2955246896 hasPublicationYear "2018" @default.
- W2955246896 type Work @default.
- W2955246896 sameAs 2955246896 @default.
- W2955246896 citedByCount "0" @default.
- W2955246896 crossrefType "journal-article" @default.
- W2955246896 hasAuthorship W2955246896A5022298420 @default.
- W2955246896 hasAuthorship W2955246896A5024611841 @default.
- W2955246896 hasAuthorship W2955246896A5044471451 @default.
- W2955246896 hasAuthorship W2955246896A5052138076 @default.
- W2955246896 hasAuthorship W2955246896A5059724032 @default.
- W2955246896 hasAuthorship W2955246896A5067084483 @default.
- W2955246896 hasAuthorship W2955246896A5076647536 @default.
- W2955246896 hasAuthorship W2955246896A5091052718 @default.
- W2955246896 hasConcept C104317684 @default.
- W2955246896 hasConcept C141231307 @default.
- W2955246896 hasConcept C142796444 @default.
- W2955246896 hasConcept C15151743 @default.
- W2955246896 hasConcept C172459030 @default.
- W2955246896 hasConcept C18903297 @default.
- W2955246896 hasConcept C2776989901 @default.
- W2955246896 hasConcept C2777385719 @default.
- W2955246896 hasConcept C2777907754 @default.
- W2955246896 hasConcept C2780892065 @default.
- W2955246896 hasConcept C3742359 @default.
- W2955246896 hasConcept C42062724 @default.
- W2955246896 hasConcept C54355233 @default.
- W2955246896 hasConcept C86803240 @default.
- W2955246896 hasConceptScore W2955246896C104317684 @default.
- W2955246896 hasConceptScore W2955246896C141231307 @default.
- W2955246896 hasConceptScore W2955246896C142796444 @default.
- W2955246896 hasConceptScore W2955246896C15151743 @default.
- W2955246896 hasConceptScore W2955246896C172459030 @default.
- W2955246896 hasConceptScore W2955246896C18903297 @default.
- W2955246896 hasConceptScore W2955246896C2776989901 @default.
- W2955246896 hasConceptScore W2955246896C2777385719 @default.
- W2955246896 hasConceptScore W2955246896C2777907754 @default.
- W2955246896 hasConceptScore W2955246896C2780892065 @default.
- W2955246896 hasConceptScore W2955246896C3742359 @default.
- W2955246896 hasConceptScore W2955246896C42062724 @default.
- W2955246896 hasConceptScore W2955246896C54355233 @default.
- W2955246896 hasConceptScore W2955246896C86803240 @default.
- W2955246896 hasLocation W29552468961 @default.
- W2955246896 hasOpenAccess W2955246896 @default.
- W2955246896 hasPrimaryLocation W29552468961 @default.
- W2955246896 hasRelatedWork W1995070372 @default.
- W2955246896 hasRelatedWork W1997985233 @default.
- W2955246896 hasRelatedWork W2032670022 @default.
- W2955246896 hasRelatedWork W2033398182 @default.
- W2955246896 hasRelatedWork W2075073024 @default.
- W2955246896 hasRelatedWork W2092451278 @default.
- W2955246896 hasRelatedWork W2124737261 @default.
- W2955246896 hasRelatedWork W2147094941 @default.
- W2955246896 hasRelatedWork W2171706806 @default.
- W2955246896 hasRelatedWork W269015836 @default.
- W2955246896 hasRelatedWork W2773228426 @default.
- W2955246896 hasRelatedWork W2803278511 @default.
- W2955246896 hasRelatedWork W2805081581 @default.
- W2955246896 hasRelatedWork W2890727456 @default.
- W2955246896 hasRelatedWork W2915106348 @default.
- W2955246896 hasRelatedWork W2942720745 @default.
- W2955246896 hasRelatedWork W2992628624 @default.
- W2955246896 hasRelatedWork W3022767408 @default.
- W2955246896 hasRelatedWork W3035397899 @default.
- W2955246896 hasRelatedWork W929925792 @default.
- W2955246896 isParatext "false" @default.
- W2955246896 isRetracted "false" @default.
- W2955246896 magId "2955246896" @default.
- W2955246896 workType "article" @default.