Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955254811> ?p ?o ?g. }
- W2955254811 endingPage "3222" @default.
- W2955254811 startingPage "3222" @default.
- W2955254811 abstract "Interleukin-13 (IL-13) drives symptoms in asthma with high levels of T-helper type 2 cells (Th2-cells). Since tight junctions (TJ) constitute the epithelial diffusion barrier, we investigated the effect of IL-13 on TJ in human tracheal epithelial cells. We observed that IL-13 increases paracellular permeability, changes claudin expression pattern and induces intracellular aggregation of the TJ proteins zonlua occludens protein 1, as well as claudins. Furthermore, IL-13 treatment increases expression of ubiquitin conjugating E2 enzyme UBE2Z. Co-localization and proximity ligation assays further showed that ubiquitin and the proteasomal marker PSMA5 co-localize with TJ proteins in IL-13 treated cells, showing that TJ proteins are ubiquitinated following IL-13 exposure. UBE2Z upregulation occurs within the first day after IL-13 exposure. Proteasomal aggregation of ubiquitinated TJ proteins starts three days after IL-13 exposure and transepithelial electrical resistance (TEER) decrease follows the time course of TJ-protein aggregation. Inhibition of JAK/STAT signaling abolishes IL-13 induced effects. Our data suggest that that IL-13 induces ubiquitination and proteasomal aggregation of TJ proteins via JAK/STAT dependent expression of UBE2Z, resulting in opening of TJs. This may contribute to barrier disturbances in pulmonary epithelia and lung damage of patients with inflammatory lung diseases." @default.
- W2955254811 created "2019-07-12" @default.
- W2955254811 creator A5006030489 @default.
- W2955254811 creator A5015246499 @default.
- W2955254811 creator A5027474720 @default.
- W2955254811 creator A5036645974 @default.
- W2955254811 creator A5050500693 @default.
- W2955254811 creator A5054968820 @default.
- W2955254811 creator A5057799813 @default.
- W2955254811 creator A5063899818 @default.
- W2955254811 creator A5064913810 @default.
- W2955254811 creator A5068639127 @default.
- W2955254811 date "2019-06-30" @default.
- W2955254811 modified "2023-10-18" @default.
- W2955254811 title "IL-13 Impairs Tight Junctions in Airway Epithelia" @default.
- W2955254811 cites W1095614472 @default.
- W2955254811 cites W1489307431 @default.
- W2955254811 cites W1506450640 @default.
- W2955254811 cites W1507367785 @default.
- W2955254811 cites W1507607612 @default.
- W2955254811 cites W1524992328 @default.
- W2955254811 cites W1963695792 @default.
- W2955254811 cites W1967056268 @default.
- W2955254811 cites W1969109349 @default.
- W2955254811 cites W1969354081 @default.
- W2955254811 cites W1979207410 @default.
- W2955254811 cites W1981985646 @default.
- W2955254811 cites W1989173007 @default.
- W2955254811 cites W1991380396 @default.
- W2955254811 cites W1994408278 @default.
- W2955254811 cites W2000614715 @default.
- W2955254811 cites W2008246770 @default.
- W2955254811 cites W2008731758 @default.
- W2955254811 cites W2016041603 @default.
- W2955254811 cites W2020328354 @default.
- W2955254811 cites W2021889502 @default.
- W2955254811 cites W2022690300 @default.
- W2955254811 cites W2034000631 @default.
- W2955254811 cites W2041225773 @default.
- W2955254811 cites W2044942673 @default.
- W2955254811 cites W2045177989 @default.
- W2955254811 cites W2054249334 @default.
- W2955254811 cites W2060763719 @default.
- W2955254811 cites W2064993319 @default.
- W2955254811 cites W2066633143 @default.
- W2955254811 cites W2067324660 @default.
- W2955254811 cites W2068360914 @default.
- W2955254811 cites W2071191421 @default.
- W2955254811 cites W2076159058 @default.
- W2955254811 cites W2079645940 @default.
- W2955254811 cites W2091307474 @default.
- W2955254811 cites W2092582603 @default.
- W2955254811 cites W2093852073 @default.
- W2955254811 cites W2096299453 @default.
- W2955254811 cites W2104197250 @default.
- W2955254811 cites W2108244474 @default.
- W2955254811 cites W2124177183 @default.
- W2955254811 cites W2124566107 @default.
- W2955254811 cites W2128472414 @default.
- W2955254811 cites W2140410130 @default.
- W2955254811 cites W2141977434 @default.
- W2955254811 cites W2147164306 @default.
- W2955254811 cites W2147339916 @default.
- W2955254811 cites W2148325247 @default.
- W2955254811 cites W2149189075 @default.
- W2955254811 cites W2150222712 @default.
- W2955254811 cites W2150814613 @default.
- W2955254811 cites W2151480919 @default.
- W2955254811 cites W2153300494 @default.
- W2955254811 cites W2158210401 @default.
- W2955254811 cites W2163087340 @default.
- W2955254811 cites W2164352733 @default.
- W2955254811 cites W2164797945 @default.
- W2955254811 cites W2293361526 @default.
- W2955254811 cites W229541132 @default.
- W2955254811 cites W2309469789 @default.
- W2955254811 cites W2320593216 @default.
- W2955254811 cites W2338240784 @default.
- W2955254811 cites W2368044794 @default.
- W2955254811 cites W2514711301 @default.
- W2955254811 cites W2554220744 @default.
- W2955254811 cites W2561979978 @default.
- W2955254811 cites W2562179046 @default.
- W2955254811 cites W2579904589 @default.
- W2955254811 cites W2591996381 @default.
- W2955254811 cites W2604513881 @default.
- W2955254811 cites W2608635043 @default.
- W2955254811 cites W2609669805 @default.
- W2955254811 cites W2681594637 @default.
- W2955254811 cites W2740279901 @default.
- W2955254811 cites W2759338004 @default.
- W2955254811 cites W2759775136 @default.
- W2955254811 cites W2766178360 @default.
- W2955254811 cites W2898141577 @default.
- W2955254811 cites W2899621095 @default.
- W2955254811 doi "https://doi.org/10.3390/ijms20133222" @default.
- W2955254811 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6651493" @default.
- W2955254811 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31262043" @default.