FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955298745> ?p ?o ?g. }

• W2955298745 endingPage "68" @default.
• W2955298745 startingPage "60" @default.
• W2955298745 abstract "The apical membrane antigen 1 (AMA-1) is a protein of the micronemes that is present in all organisms of the phylum Apicomplexa; it has been shown that AMA-1 plays an essential role for parasite invasion to target cells. It has been reported that AMA-1 is conserved among different isolates of Babesia; however, it is unknown whether the protein contains conserved B-cell epitopes and whether these epitopes are recognized by antibodies from cattle in endemic areas. In this research, using an in silico analysis, four peptides were designed containing exposed and conserved linear B-cell epitopes from the extracellular region of Babesia bovis AMA-1. The selected peptides were chemically synthesized, and then each peptide was emulsified and used to immunize two bovines per peptide. The antibodies produced against these peptides were able to recognize intra-erythrocytic parasites in an IFAT, except peptide 4, which was insoluble. The synthetic peptides were covalently fixed to the wells of an ELISA plate and incubated with sera from B. bovis naturally infected cattle. Peptides P2AMA and P3AMA were recognized by the sera of naturally infected cattle from different regions of Mexico. Statistical analysis showed that the ELISA test for peptides P2AMA and P3AMA had a concordance of 91.2% and 61.1% compared to the IFAT, a sensitivity of 94.56% and 71.74%, and a specificity of 76.19% and 14.2%, respectively. The presence of antibodies in bovine sera from endemic areas that bind to the identified peptides indicates that AMA-1 from B. bovis has conserved B-cell epitopes involved in the immune response under natural conditions. However, to propose their use as vaccine or diagnostics candidates, a further characterization of the humoral immune response elicited in cattle by these peptides is needed." @default.
• W2955298745 created "2019-07-12" @default.
• W2955298745 creator A5026254981 @default.
• W2955298745 creator A5028007333 @default.
• W2955298745 creator A5052165632 @default.
• W2955298745 creator A5064603712 @default.
• W2955298745 creator A5078743375 @default.
• W2955298745 creator A5084647168 @default.
• W2955298745 date "2019-06-24" @default.
• W2955298745 modified "2023-10-01" @default.
• W2955298745 title "Identification of conserved peptides containing B‐cell epitopes of <i>Babesia bovis</i> <scp>AMA</scp> ‐1 and their potential as diagnostics candidates" @default.
• W2955298745 cites W1876869943 @default.
• W2955298745 cites W1905502445 @default.
• W2955298745 cites W1964052312 @default.
• W2955298745 cites W1966867282 @default.
• W2955298745 cites W1968577069 @default.
• W2955298745 cites W1971054074 @default.
• W2955298745 cites W1973560378 @default.
• W2955298745 cites W1974483800 @default.
• W2955298745 cites W1981024153 @default.
• W2955298745 cites W1985814731 @default.
• W2955298745 cites W1989586488 @default.
• W2955298745 cites W2003990171 @default.
• W2955298745 cites W2007270997 @default.
• W2955298745 cites W2019078796 @default.
• W2955298745 cites W2020471029 @default.
• W2955298745 cites W2022538161 @default.
• W2955298745 cites W2032178409 @default.
• W2955298745 cites W2048970223 @default.
• W2955298745 cites W2051885116 @default.
• W2955298745 cites W2052257222 @default.
• W2955298745 cites W2069460651 @default.
• W2955298745 cites W2078641301 @default.
• W2955298745 cites W2085750319 @default.
• W2955298745 cites W2088677483 @default.
• W2955298745 cites W2090387688 @default.
• W2955298745 cites W2105557390 @default.
• W2955298745 cites W2111869141 @default.
• W2955298745 cites W2119892123 @default.
• W2955298745 cites W2120045745 @default.
• W2955298745 cites W2120169200 @default.
• W2955298745 cites W2133627748 @default.
• W2955298745 cites W2133704481 @default.
• W2955298745 cites W2139968244 @default.
• W2955298745 cites W2153756387 @default.
• W2955298745 cites W2162413693 @default.
• W2955298745 cites W2162518989 @default.
• W2955298745 cites W2400954299 @default.
• W2955298745 cites W2789620669 @default.
• W2955298745 cites W2899256563 @default.
• W2955298745 doi "https://doi.org/10.1111/tbed.13213" @default.
• W2955298745 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31231975" @default.
• W2955298745 hasPublicationYear "2019" @default.
• W2955298745 type Work @default.
• W2955298745 sameAs 2955298745 @default.
• W2955298745 citedByCount "3" @default.
• W2955298745 countsByYear W29552987452021 @default.
• W2955298745 countsByYear W29552987452022 @default.
• W2955298745 countsByYear W29552987452023 @default.
• W2955298745 crossrefType "journal-article" @default.
• W2955298745 hasAuthorship W2955298745A5026254981 @default.
• W2955298745 hasAuthorship W2955298745A5028007333 @default.
• W2955298745 hasAuthorship W2955298745A5052165632 @default.
• W2955298745 hasAuthorship W2955298745A5064603712 @default.
• W2955298745 hasAuthorship W2955298745A5078743375 @default.
• W2955298745 hasAuthorship W2955298745A5084647168 @default.
• W2955298745 hasBestOaLocation W29552987451 @default.
• W2955298745 hasConcept C104317684 @default.
• W2955298745 hasConcept C136764020 @default.
• W2955298745 hasConcept C147483822 @default.
• W2955298745 hasConcept C153911025 @default.
• W2955298745 hasConcept C159047783 @default.
• W2955298745 hasConcept C159654299 @default.
• W2955298745 hasConcept C195616568 @default.
• W2955298745 hasConcept C203014093 @default.
• W2955298745 hasConcept C2775905019 @default.
• W2955298745 hasConcept C2777119855 @default.
• W2955298745 hasConcept C2779281246 @default.
• W2955298745 hasConcept C2780581805 @default.
• W2955298745 hasConcept C41008148 @default.
• W2955298745 hasConcept C55493867 @default.
• W2955298745 hasConcept C71928629 @default.
• W2955298745 hasConcept C86803240 @default.
• W2955298745 hasConceptScore W2955298745C104317684 @default.
• W2955298745 hasConceptScore W2955298745C136764020 @default.
• W2955298745 hasConceptScore W2955298745C147483822 @default.
• W2955298745 hasConceptScore W2955298745C153911025 @default.
• W2955298745 hasConceptScore W2955298745C159047783 @default.
• W2955298745 hasConceptScore W2955298745C159654299 @default.
• W2955298745 hasConceptScore W2955298745C195616568 @default.
• W2955298745 hasConceptScore W2955298745C203014093 @default.
• W2955298745 hasConceptScore W2955298745C2775905019 @default.
• W2955298745 hasConceptScore W2955298745C2777119855 @default.
• W2955298745 hasConceptScore W2955298745C2779281246 @default.
• W2955298745 hasConceptScore W2955298745C2780581805 @default.
• W2955298745 hasConceptScore W2955298745C41008148 @default.
• W2955298745 hasConceptScore W2955298745C55493867 @default.
• W2955298745 hasConceptScore W2955298745C71928629 @default.

• next