FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955416986> ?p ?o ?g. }

• W2955416986 abstract "The aim of the current study was to prepare and identify mouse anti-human rotavirus (RV) VP7 monoclonal antibodies and explore their protective effects on RV infection. The mouse anti-human RV VP7 monoclonal antibody was produced using the ascites method and identified via western blot analysis. In vitro neutralization of mouse anti-human RV VP7 monoclonal antibodies was detected by performing an MTT assay. The TCID50 value was calculated to obtain antibody neutralization titers. A mouse RV infection model was generated to assess the protective effect of the mouse anti-human RV VP7 monoclonal antibody in experimental animals. Monoclonal antibodies were successfully prepared and their purity reached ≥90%. Western blotting demonstrated that monoclonal antibodies specifically bound to the purified Wa RV strain, with a specific reaction band at ~40 kDa. Monoclonal antibody in vitro neutralization results demonstrated that cell survival rate in the virus + monoclonal antibody group was higher than that in virus + maintenance fluid group (P<0.05). Monoclonal antibody neutralization titer detection revealed that the cytopathic effect did not extend beyond 4 days. In addition, the calculated monoclonal antibody neutralization titer was 1:446. The results revealed that the positive rate of colloidal gold RV in the 100 µl monoclonal antibody group was significantly lower than that in the control group (P<0.05). Furthermore, the protection rate of the 100 µl monoclonal antibody group was 71.4%, whereas the 50 µl monoclonal antibody group was 42.9% and the ribavirin group was 57.1%. In conclusion, the results of the current study demonstrated that mouse anti-human RV VP7 monoclonal antibodies can be successfully prepared using ascites method. These antibodies also effectively neutralize the cytotoxic effects of the human RV Wa strain in vitro and mouse anti-human RV VP7 monoclonal antibodies also exhibited a good protective role in mice. Furthermore, greater protective effects were observed at a higher dose and the protective effects of these high dose treatments were superior to that of ribavirin." @default.
• W2955416986 created "2019-07-12" @default.
• W2955416986 creator A5000312885 @default.
• W2955416986 creator A5002930476 @default.
• W2955416986 creator A5007681919 @default.
• W2955416986 creator A5013758739 @default.
• W2955416986 creator A5020663305 @default.
• W2955416986 creator A5030376299 @default.
• W2955416986 creator A5032539923 @default.
• W2955416986 creator A5045202428 @default.
• W2955416986 creator A5048784008 @default.
• W2955416986 creator A5050294547 @default.
• W2955416986 date "2019-06-25" @default.
• W2955416986 modified "2023-09-28" @default.
• W2955416986 title "Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection" @default.
• W2955416986 cites W1457117875 @default.
• W2955416986 cites W1460528919 @default.
• W2955416986 cites W148302497 @default.
• W2955416986 cites W1604596271 @default.
• W2955416986 cites W1995032066 @default.
• W2955416986 cites W2005089810 @default.
• W2955416986 cites W2007492632 @default.
• W2955416986 cites W2009204638 @default.
• W2955416986 cites W2029975353 @default.
• W2955416986 cites W2033830055 @default.
• W2955416986 cites W2050909685 @default.
• W2955416986 cites W2059574711 @default.
• W2955416986 cites W2084847486 @default.
• W2955416986 cites W2109554224 @default.
• W2955416986 cites W2111839663 @default.
• W2955416986 cites W2125466256 @default.
• W2955416986 cites W2142646071 @default.
• W2955416986 cites W2150560479 @default.
• W2955416986 cites W2155013736 @default.
• W2955416986 cites W2177044881 @default.
• W2955416986 cites W2188300753 @default.
• W2955416986 cites W2328659162 @default.
• W2955416986 cites W2409546644 @default.
• W2955416986 cites W2410969804 @default.
• W2955416986 cites W2415987610 @default.
• W2955416986 cites W2626184818 @default.
• W2955416986 cites W2734698011 @default.
• W2955416986 cites W2763897213 @default.
• W2955416986 cites W2800135369 @default.
• W2955416986 cites W2801863874 @default.
• W2955416986 cites W2807982539 @default.
• W2955416986 cites W2884966882 @default.
• W2955416986 cites W2904065473 @default.
• W2955416986 cites W427184505 @default.
• W2955416986 cites W622804064 @default.
• W2955416986 cites W68353136 @default.
• W2955416986 cites W93380844 @default.
• W2955416986 doi "https://doi.org/10.3892/etm.2019.7708" @default.
• W2955416986 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6639772" @default.
• W2955416986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31384336" @default.
• W2955416986 hasPublicationYear "2019" @default.
• W2955416986 type Work @default.
• W2955416986 sameAs 2955416986 @default.
• W2955416986 citedByCount "2" @default.
• W2955416986 countsByYear W29554169862022 @default.
• W2955416986 countsByYear W29554169862023 @default.
• W2955416986 crossrefType "journal-article" @default.
• W2955416986 hasAuthorship W2955416986A5000312885 @default.
• W2955416986 hasAuthorship W2955416986A5002930476 @default.
• W2955416986 hasAuthorship W2955416986A5007681919 @default.
• W2955416986 hasAuthorship W2955416986A5013758739 @default.
• W2955416986 hasAuthorship W2955416986A5020663305 @default.
• W2955416986 hasAuthorship W2955416986A5030376299 @default.
• W2955416986 hasAuthorship W2955416986A5032539923 @default.
• W2955416986 hasAuthorship W2955416986A5045202428 @default.
• W2955416986 hasAuthorship W2955416986A5048784008 @default.
• W2955416986 hasAuthorship W2955416986A5050294547 @default.
• W2955416986 hasBestOaLocation W29554169861 @default.
• W2955416986 hasConcept C104317684 @default.
• W2955416986 hasConcept C14086860 @default.
• W2955416986 hasConcept C153911025 @default.
• W2955416986 hasConcept C159047783 @default.
• W2955416986 hasConcept C159654299 @default.
• W2955416986 hasConcept C163864487 @default.
• W2955416986 hasConcept C203014093 @default.
• W2955416986 hasConcept C2522874641 @default.
• W2955416986 hasConcept C2776415932 @default.
• W2955416986 hasConcept C2781367151 @default.
• W2955416986 hasConcept C32611913 @default.
• W2955416986 hasConcept C542903549 @default.
• W2955416986 hasConcept C55493867 @default.
• W2955416986 hasConcept C86803240 @default.
• W2955416986 hasConceptScore W2955416986C104317684 @default.
• W2955416986 hasConceptScore W2955416986C14086860 @default.
• W2955416986 hasConceptScore W2955416986C153911025 @default.
• W2955416986 hasConceptScore W2955416986C159047783 @default.
• W2955416986 hasConceptScore W2955416986C159654299 @default.
• W2955416986 hasConceptScore W2955416986C163864487 @default.
• W2955416986 hasConceptScore W2955416986C203014093 @default.
• W2955416986 hasConceptScore W2955416986C2522874641 @default.
• W2955416986 hasConceptScore W2955416986C2776415932 @default.
• W2955416986 hasConceptScore W2955416986C2781367151 @default.
• W2955416986 hasConceptScore W2955416986C32611913 @default.
• W2955416986 hasConceptScore W2955416986C542903549 @default.
• W2955416986 hasConceptScore W2955416986C55493867 @default.

• next