FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955493014> ?p ?o ?g. }

• W2955493014 endingPage "218" @default.
• W2955493014 startingPage "217" @default.
• W2955493014 abstract "Nanotechnology is driving scientists to better comprehend the real‐time dynamics and structure‐property relationship of various materials and biological samples under liquid conditions. Such understanding is crucial for a wide range of applications involving, for example, nanoparticle synthesis, self‐assembly processes, (bio) molecular interactions, and biological activity in cells. In‐situ transmission electron microscopy (TEM) observations in the liquid‐phase is expected to lead to better scientific understanding, the discovery of phenomena at the nanoscale in liquid not visible before, and results in novel and innovative applications. Here we present the development of the “Ocean System”, which is an easy‐to‐use add‐on that enables in‐situ liquid studies inside the TEM (Figure 1). It consists of an optimized TEM holder that uses a microfluidic chamber as sample carrier, replacing the traditional copper grid. Such device, referred to as Nano‐Cell, acts as a multi‐functional and micro‐sized laboratory that keeps the sample in a fully hydrated state. Furthermore, the system includes an external test station that guarantees the safe loading of the holder into the TEM. Each Nano‐Cell consists of two chips (Figure 2) that are sandwiched together to form a sealed microfluidic compartment. Both chips are covered with silicon nitride providing an electron transparent window and ensuring their chemical inertness and biocompatibility. Samples are prepared directly onto the electron transparent windows, which allow for the electron beam to pass through for in‐situ imaging. Biological cells can also be directly grown on the chips. In order to control the liquid thickness to improve imaging resolution, the experiment can be customized by selecting the best‐suited spacer based on sample size. Having direct access to the electron transparent windows enables local functionalization of the membrane's surface, empowering the user to further control the microfluidic environment. The holder tip contains a precision slot with various alignment poles that ensure self‐alignment of the top and bottom chips. Similarly, it contains a by‐pass structure that prevents overpressures during liquid handling, and that allows rapid liquid exchange in the tubing, since the flow cross‐section in this channel is much larger than that of the liquid path between the chips. The tip closure mechanism uses alignment balls, so that the tip correctly closes when screws are tightened independently on the applied force. The mechanism prevents over‐compression of the O‐rings and ensures that no shear stress will be transferred to the Nano‐Cell, as these could damage fragile samples (i.e. biological cell). Additionally, the modular design ensures reliable results with easy replacement of all holder parts, such as tubing, holder tip and the Nano‐Cell (Figure 3). This is particularly important, as it prevents cross‐contamination between different experiments, and the tubing can be easily replaced by the user if these become clogged. In addition, the tip can be rotated by 180°, so that depending if one wants to use TEM or STEM, the optimal resolution can be achieved for the sample, i.e. TEM achieves the highest resolution for objects below a liquid layer for a downward traveling electron beam, while the opposite is true for STEM. The Ocean System can be used to study dynamic processes of nanoparticles. E.g. gold nanoparticles can be loosely attached to a SiN membrane. Their detachment during the experiment can be triggered by increasing the induced electron dose. This can provide useful information such as the interaction of nanoobjects (e.g. agglomeration, self‐assembly, sintering) in different liquids. Figure 4 shows Au nanoparticles being attached to the SiN membrane. Upon imaging at higher magnifications the nanoparticles start moving along the SiN membrane and start to form agglomerates. Particle tracking was applied to 4 selected Au nanoparticles to study their movement." @default.
• W2955493014 created "2019-07-12" @default.
• W2955493014 creator A5001678663 @default.
• W2955493014 creator A5005785723 @default.
• W2955493014 creator A5020079500 @default.
• W2955493014 creator A5045853646 @default.
• W2955493014 creator A5055308297 @default.
• W2955493014 creator A5062984209 @default.
• W2955493014 creator A5066670637 @default.
• W2955493014 creator A5069969018 @default.
• W2955493014 date "2016-12-20" @default.
• W2955493014 modified "2023-10-16" @default.
• W2955493014 title "The “Ocean” System: Microfluidic based system for in‐situ analysis of liquid processes inside the <scp>TEM</scp>" @default.
• W2955493014 doi "https://doi.org/10.1002/9783527808465.emc2016.6642" @default.
• W2955493014 hasPublicationYear "2016" @default.
• W2955493014 type Work @default.
• W2955493014 sameAs 2955493014 @default.
• W2955493014 citedByCount "0" @default.
• W2955493014 crossrefType "other" @default.
• W2955493014 hasAuthorship W2955493014A5001678663 @default.
• W2955493014 hasAuthorship W2955493014A5005785723 @default.
• W2955493014 hasAuthorship W2955493014A5020079500 @default.
• W2955493014 hasAuthorship W2955493014A5045853646 @default.
• W2955493014 hasAuthorship W2955493014A5055308297 @default.
• W2955493014 hasAuthorship W2955493014A5062984209 @default.
• W2955493014 hasAuthorship W2955493014A5066670637 @default.
• W2955493014 hasAuthorship W2955493014A5069969018 @default.
• W2955493014 hasBestOaLocation W29554930141 @default.
• W2955493014 hasConcept C146088050 @default.
• W2955493014 hasConcept C155672457 @default.
• W2955493014 hasConcept C171250308 @default.
• W2955493014 hasConcept C178790620 @default.
• W2955493014 hasConcept C185592680 @default.
• W2955493014 hasConcept C192562407 @default.
• W2955493014 hasConcept C2777822432 @default.
• W2955493014 hasConcept C2780841128 @default.
• W2955493014 hasConcept C8673954 @default.
• W2955493014 hasConceptScore W2955493014C146088050 @default.
• W2955493014 hasConceptScore W2955493014C155672457 @default.
• W2955493014 hasConceptScore W2955493014C171250308 @default.
• W2955493014 hasConceptScore W2955493014C178790620 @default.
• W2955493014 hasConceptScore W2955493014C185592680 @default.
• W2955493014 hasConceptScore W2955493014C192562407 @default.
• W2955493014 hasConceptScore W2955493014C2777822432 @default.
• W2955493014 hasConceptScore W2955493014C2780841128 @default.
• W2955493014 hasConceptScore W2955493014C8673954 @default.
• W2955493014 hasLocation W29554930141 @default.
• W2955493014 hasOpenAccess W2955493014 @default.
• W2955493014 hasPrimaryLocation W29554930141 @default.
• W2955493014 hasRelatedWork W2023829906 @default.
• W2955493014 hasRelatedWork W2029557108 @default.
• W2955493014 hasRelatedWork W2737498735 @default.
• W2955493014 hasRelatedWork W2894312583 @default.
• W2955493014 hasRelatedWork W3012559914 @default.
• W2955493014 hasRelatedWork W3138316926 @default.
• W2955493014 hasRelatedWork W3207861065 @default.
• W2955493014 hasRelatedWork W4243387708 @default.
• W2955493014 hasRelatedWork W4249938786 @default.
• W2955493014 hasRelatedWork W4362557324 @default.
• W2955493014 isParatext "false" @default.
• W2955493014 isRetracted "false" @default.
• W2955493014 magId "2955493014" @default.
• W2955493014 workType "other" @default.