Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955513904> ?p ?o ?g. }
- W2955513904 abstract "Abstract The retinal pigment epithelium (RPE) supports the outer retina through essential roles in the retinoid the visual cycle, nutrient supply, ion exchange and waste removal. Each day the RPE removes the oldest ∼10% of photoreceptor outer segments through phagocytic uptake, which peaks in a synchronous burst following light onset. Impaired degradation of phagocytosed OS material by the RPE can lead to toxic accumulation of lipids, oxidative tissue damage, inflammation and cell death. OSs are rich in very long chain fatty acids which are preferentially catabolized in peroxisomes. Despite the importance of lipid degradation in RPE function, the regulation of peroxisome number and activity relative to diurnal OS ingestion is relatively unexplored. Using immunohistochemistry, immunoblotting and catalase activity assays, we investigated peroxisome abundance and activity at 6 am, 7 am (at lights on), 8 am, and 3 pm, in WT mice and mice lacking microtubule-associated protein 1 light chain 3B (LC3B), that have impaired degradation of phagosomes. We found that catalase activity, but not protein expression, is 50% higher in the morning compared with 3 pm, in RPE of WT but not LC3B -/- mice. Surprisingly, we found that peroxisome abundance was stable during the day, however numbers are elevated overall in LC3B -/- mice, implicating LC3B in autophagic organelle turnover in RPE. Our data suggest that RPE peroxisome function is regulated in coordination with phagocytosis, possibly through direct enzyme regulation, and may serve to prepare RPE peroxisomes for daily surges in ingested lipid-rich OS." @default.
- W2955513904 created "2019-07-12" @default.
- W2955513904 creator A5039751103 @default.
- W2955513904 creator A5041046118 @default.
- W2955513904 creator A5044008862 @default.
- W2955513904 creator A5051841096 @default.
- W2955513904 creator A5071898431 @default.
- W2955513904 creator A5075109208 @default.
- W2955513904 creator A5077453291 @default.
- W2955513904 creator A5090087970 @default.
- W2955513904 date "2019-07-01" @default.
- W2955513904 modified "2023-10-13" @default.
- W2955513904 title "Peroxisome turnover and diurnal modulation of antioxidant activity in retinal pigment epithelia utilizes microtubule-associated protein 1 light chain 3B" @default.
- W2955513904 cites W1553889001 @default.
- W2955513904 cites W1796307613 @default.
- W2955513904 cites W1838356724 @default.
- W2955513904 cites W1846402700 @default.
- W2955513904 cites W1875936957 @default.
- W2955513904 cites W1963591622 @default.
- W2955513904 cites W1965097583 @default.
- W2955513904 cites W1978462231 @default.
- W2955513904 cites W1978810668 @default.
- W2955513904 cites W1987601583 @default.
- W2955513904 cites W1994133882 @default.
- W2955513904 cites W1994965575 @default.
- W2955513904 cites W2010381561 @default.
- W2955513904 cites W2010648114 @default.
- W2955513904 cites W2013238661 @default.
- W2955513904 cites W2017759854 @default.
- W2955513904 cites W2021163002 @default.
- W2955513904 cites W2030131046 @default.
- W2955513904 cites W2032241410 @default.
- W2955513904 cites W2032391189 @default.
- W2955513904 cites W2035245572 @default.
- W2955513904 cites W2035724742 @default.
- W2955513904 cites W2044525934 @default.
- W2955513904 cites W2045834652 @default.
- W2955513904 cites W2051744916 @default.
- W2955513904 cites W2051747007 @default.
- W2955513904 cites W2060387874 @default.
- W2955513904 cites W2061013234 @default.
- W2955513904 cites W2066839216 @default.
- W2955513904 cites W2068551753 @default.
- W2955513904 cites W2069594369 @default.
- W2955513904 cites W2082211576 @default.
- W2955513904 cites W2082484702 @default.
- W2955513904 cites W2090663997 @default.
- W2955513904 cites W2092070727 @default.
- W2955513904 cites W2092348954 @default.
- W2955513904 cites W2093956295 @default.
- W2955513904 cites W2094764360 @default.
- W2955513904 cites W2096494569 @default.
- W2955513904 cites W2105422846 @default.
- W2955513904 cites W2105465142 @default.
- W2955513904 cites W2105817940 @default.
- W2955513904 cites W2112744358 @default.
- W2955513904 cites W2113593409 @default.
- W2955513904 cites W2114108892 @default.
- W2955513904 cites W2114914263 @default.
- W2955513904 cites W2115343507 @default.
- W2955513904 cites W2115956491 @default.
- W2955513904 cites W2122935607 @default.
- W2955513904 cites W2127701223 @default.
- W2955513904 cites W2129321855 @default.
- W2955513904 cites W2147481857 @default.
- W2955513904 cites W2152977496 @default.
- W2955513904 cites W2155385796 @default.
- W2955513904 cites W2160550697 @default.
- W2955513904 cites W2209734561 @default.
- W2955513904 cites W2250849461 @default.
- W2955513904 cites W2299041215 @default.
- W2955513904 cites W2330533782 @default.
- W2955513904 cites W2416561108 @default.
- W2955513904 cites W2484914234 @default.
- W2955513904 cites W2554675960 @default.
- W2955513904 cites W2555734739 @default.
- W2955513904 cites W2569601267 @default.
- W2955513904 cites W2595778161 @default.
- W2955513904 cites W2606699511 @default.
- W2955513904 cites W2772060643 @default.
- W2955513904 cites W2800532708 @default.
- W2955513904 cites W2803977908 @default.
- W2955513904 cites W2804206940 @default.
- W2955513904 cites W2897599900 @default.
- W2955513904 cites W4244687418 @default.
- W2955513904 doi "https://doi.org/10.1101/687533" @default.
- W2955513904 hasPublicationYear "2019" @default.
- W2955513904 type Work @default.
- W2955513904 sameAs 2955513904 @default.
- W2955513904 citedByCount "0" @default.
- W2955513904 crossrefType "posted-content" @default.
- W2955513904 hasAuthorship W2955513904A5039751103 @default.
- W2955513904 hasAuthorship W2955513904A5041046118 @default.
- W2955513904 hasAuthorship W2955513904A5044008862 @default.
- W2955513904 hasAuthorship W2955513904A5051841096 @default.
- W2955513904 hasAuthorship W2955513904A5071898431 @default.
- W2955513904 hasAuthorship W2955513904A5075109208 @default.
- W2955513904 hasAuthorship W2955513904A5077453291 @default.
- W2955513904 hasAuthorship W2955513904A5090087970 @default.
- W2955513904 hasBestOaLocation W29555139041 @default.