FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955577047> ?p ?o ?g. }

• W2955577047 endingPage "1887" @default.
• W2955577047 startingPage "1881" @default.
• W2955577047 abstract "Nowadays, Listeria monocytogenes continues to be a major health issue. Therefore, improvements in the speed and reliability of its detection are still needed. In the present study, the combination of real-time Recombinase Polymerase Amplification (qRPA) with immunomagnetic separation (IMS) is described. The proposed methodology was tested against a real-time PCR method, and was successfully applied to 50 smoked salmon samples spiked at levels ranging from 2 to 9.3 × 102 cfu/25 g. L. monocytogenes was detected after a 24 hr pre-enrichment, which represents a great improvement over other previously published RPA methods. Additionally, the evaluation of the method reported a Limit of dDetection 50 (LoD50 ) of 6.3 cfu/25 g, along with relative sensitivity, specificity and accuracy values higher than 90%. Finally, the index of kappa concordance was calculated to be 0.93 which is interpreted as almost complete concordance between the reference and alternative method. Overall, the described methodology proved to be faster, specific, and as sensitive as other methods based on RPA or real-time PCR. PRACTICAL APPLICATION: The methodology described in this study significantly reduces the detection time of L. monocytogenes, when compared with culture-based methods, and it requires fewer steps than other molecular methods, making it a reliable and more convenient method for routine testing. Finally, the evaluation of the methodology in spiked food samples, confirms its reliability." @default.
• W2955577047 created "2019-07-12" @default.
• W2955577047 creator A5007093315 @default.
• W2955577047 creator A5056752498 @default.
• W2955577047 creator A5057297985 @default.
• W2955577047 creator A5066687709 @default.
• W2955577047 date "2019-07-01" @default.
• W2955577047 modified "2023-09-24" @default.
• W2955577047 title "Combination of Immunomagnetic Separation and Real‐Time Recombinase Polymerase Amplification (IMS‐qRPA) for Specific Detection of<i>Listeria monocytogenes</i>in Smoked Salmon Samples" @default.
• W2955577047 cites W1504544447 @default.
• W2955577047 cites W1514543979 @default.
• W2955577047 cites W1577762533 @default.
• W2955577047 cites W1991786586 @default.
• W2955577047 cites W1994407452 @default.
• W2955577047 cites W1996346823 @default.
• W2955577047 cites W2012903757 @default.
• W2955577047 cites W2024568511 @default.
• W2955577047 cites W2029983569 @default.
• W2955577047 cites W2032281711 @default.
• W2955577047 cites W2041287513 @default.
• W2955577047 cites W2049792325 @default.
• W2955577047 cites W2069984876 @default.
• W2955577047 cites W2072454163 @default.
• W2955577047 cites W2103740237 @default.
• W2955577047 cites W2105275554 @default.
• W2955577047 cites W2153350094 @default.
• W2955577047 cites W2162042128 @default.
• W2955577047 cites W2214933808 @default.
• W2955577047 cites W2310765798 @default.
• W2955577047 cites W2320250422 @default.
• W2955577047 cites W2343821368 @default.
• W2955577047 cites W2562719500 @default.
• W2955577047 cites W2590466406 @default.
• W2955577047 cites W2612168837 @default.
• W2955577047 cites W2765537498 @default.
• W2955577047 cites W2766341309 @default.
• W2955577047 cites W2766658376 @default.
• W2955577047 cites W2774029591 @default.
• W2955577047 cites W2780240577 @default.
• W2955577047 cites W2793155109 @default.
• W2955577047 cites W992136934 @default.
• W2955577047 doi "https://doi.org/10.1111/1750-3841.14662" @default.
• W2955577047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31264719" @default.
• W2955577047 hasPublicationYear "2019" @default.
• W2955577047 type Work @default.
• W2955577047 sameAs 2955577047 @default.
• W2955577047 citedByCount "29" @default.
• W2955577047 countsByYear W29555770472020 @default.
• W2955577047 countsByYear W29555770472021 @default.
• W2955577047 countsByYear W29555770472022 @default.
• W2955577047 countsByYear W29555770472023 @default.
• W2955577047 crossrefType "journal-article" @default.
• W2955577047 hasAuthorship W2955577047A5007093315 @default.
• W2955577047 hasAuthorship W2955577047A5056752498 @default.
• W2955577047 hasAuthorship W2955577047A5057297985 @default.
• W2955577047 hasAuthorship W2955577047A5066687709 @default.
• W2955577047 hasConcept C104317684 @default.
• W2955577047 hasConcept C119128265 @default.
• W2955577047 hasConcept C139713532 @default.
• W2955577047 hasConcept C160798450 @default.
• W2955577047 hasConcept C185592680 @default.
• W2955577047 hasConcept C2781053203 @default.
• W2955577047 hasConcept C2781350384 @default.
• W2955577047 hasConcept C43617362 @default.
• W2955577047 hasConcept C48023723 @default.
• W2955577047 hasConcept C49105822 @default.
• W2955577047 hasConcept C523546767 @default.
• W2955577047 hasConcept C54355233 @default.
• W2955577047 hasConcept C60644358 @default.
• W2955577047 hasConcept C86803240 @default.
• W2955577047 hasConceptScore W2955577047C104317684 @default.
• W2955577047 hasConceptScore W2955577047C119128265 @default.
• W2955577047 hasConceptScore W2955577047C139713532 @default.
• W2955577047 hasConceptScore W2955577047C160798450 @default.
• W2955577047 hasConceptScore W2955577047C185592680 @default.
• W2955577047 hasConceptScore W2955577047C2781053203 @default.
• W2955577047 hasConceptScore W2955577047C2781350384 @default.
• W2955577047 hasConceptScore W2955577047C43617362 @default.
• W2955577047 hasConceptScore W2955577047C48023723 @default.
• W2955577047 hasConceptScore W2955577047C49105822 @default.
• W2955577047 hasConceptScore W2955577047C523546767 @default.
• W2955577047 hasConceptScore W2955577047C54355233 @default.
• W2955577047 hasConceptScore W2955577047C60644358 @default.
• W2955577047 hasConceptScore W2955577047C86803240 @default.
• W2955577047 hasFunder F4320335322 @default.
• W2955577047 hasIssue "7" @default.
• W2955577047 hasLocation W29555770471 @default.
• W2955577047 hasLocation W29555770472 @default.
• W2955577047 hasOpenAccess W2955577047 @default.
• W2955577047 hasPrimaryLocation W29555770471 @default.
• W2955577047 hasRelatedWork W1983589436 @default.
• W2955577047 hasRelatedWork W2043990204 @default.
• W2955577047 hasRelatedWork W2049244499 @default.
• W2955577047 hasRelatedWork W2069984876 @default.
• W2955577047 hasRelatedWork W2256997635 @default.
• W2955577047 hasRelatedWork W2359417044 @default.
• W2955577047 hasRelatedWork W2406599417 @default.
• W2955577047 hasRelatedWork W2797708231 @default.

• next