FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955606500> ?p ?o ?g. }

• W2955606500 abstract "Brucella, the agent causing brucellosis, is a major zoonotic pathogen with worldwide distribution. Brucella resides and replicates inside infected host cells in membrane-bound compartments called Brucella-containing vacuoles (BCVs). Following uptake, Brucella resides in endosomal BCVs (eBCVs) that gradually mature from early to late endosomal features. Through a poorly understood process that is key to the intracellular lifestyle of Brucella, the eBCV escapes fusion with lysosomes by transitioning to the replicative BCV (rBCV), a replicative niche directly connected to the endoplasmic reticulum (ER). Despite the notion that this complex intracellular lifestyle must depend on a multitude of host factors, a holistic view on which of these components control Brucella cell entry, trafficking, and replication is still missing. Here we used a systematic cell-based small interfering RNA (siRNA) knockdown screen in HeLa cells infected with Brucella abortus and identified 425 components of the human infectome for Brucella infection. These include multiple components of pathways involved in central processes such as the cell cycle, actin cytoskeleton dynamics, or vesicular trafficking. Using assays for pathogen entry, knockdown complementation, and colocalization at single-cell resolution, we identified the requirement of the VPS retromer for Brucella to escape the lysosomal degradative pathway and to establish its intracellular replicative niche. We thus validated the VPS retromer as a novel host factor critical for Brucella intracellular trafficking. Further, our genomewide data shed light on the interplay between central host processes and the biogenesis of the Brucella replicative niche.IMPORTANCE With >300,000 new cases of human brucellosis annually, Brucella is regarded as one of the most important zoonotic bacterial pathogens worldwide. The agent causing brucellosis resides inside host cells within vacuoles termed Brucella-containing vacuoles (BCVs). Although a few host components required to escape the degradative lysosomal pathway and to establish the ER-derived replicative BCV (rBCV) have already been identified, the global understanding of this highly coordinated process is still partial, and many factors remain unknown. To gain deeper insight into these fundamental questions, we performed a genomewide RNA interference (RNAi) screen aiming at discovering novel host factors involved in the Brucella intracellular cycle. We identified 425 host proteins that contribute to Brucella cellular entry, intracellular trafficking, and replication. Together, this study sheds light on previously unknown host pathways required for the Brucella infection cycle and highlights the VPS retromer components as critical factors for the establishment of the Brucella intracellular replicative niche." @default.
• W2955606500 created "2019-07-12" @default.
• W2955606500 creator A5003790381 @default.
• W2955606500 creator A5016336211 @default.
• W2955606500 creator A5018268636 @default.
• W2955606500 creator A5018962844 @default.
• W2955606500 creator A5032772458 @default.
• W2955606500 creator A5033018473 @default.
• W2955606500 creator A5041639548 @default.
• W2955606500 creator A5052262478 @default.
• W2955606500 creator A5069299612 @default.
• W2955606500 creator A5085766621 @default.
• W2955606500 date "2019-06-26" @default.
• W2955606500 modified "2023-10-10" @default.
• W2955606500 title "A Role for the VPS Retromer in <i>Brucella</i> Intracellular Replication Revealed by Genomewide siRNA Screening" @default.
• W2955606500 cites W1516970347 @default.
• W2955606500 cites W1753222877 @default.
• W2955606500 cites W1804367334 @default.
• W2955606500 cites W1958239886 @default.
• W2955606500 cites W1968578345 @default.
• W2955606500 cites W1980103846 @default.
• W2955606500 cites W1991856556 @default.
• W2955606500 cites W2006591980 @default.
• W2955606500 cites W2006992107 @default.
• W2955606500 cites W2009275634 @default.
• W2955606500 cites W2010009494 @default.
• W2955606500 cites W2018635122 @default.
• W2955606500 cites W2018875143 @default.
• W2955606500 cites W2024235347 @default.
• W2955606500 cites W2025291938 @default.
• W2955606500 cites W2033042393 @default.
• W2955606500 cites W2035356844 @default.
• W2955606500 cites W2040615565 @default.
• W2955606500 cites W2045209612 @default.
• W2955606500 cites W2048846412 @default.
• W2955606500 cites W2050896016 @default.
• W2955606500 cites W2056133106 @default.
• W2955606500 cites W2063129130 @default.
• W2955606500 cites W2079911954 @default.
• W2955606500 cites W2083329781 @default.
• W2955606500 cites W2093637310 @default.
• W2955606500 cites W2098166937 @default.
• W2955606500 cites W2098478833 @default.
• W2955606500 cites W2102121381 @default.
• W2955606500 cites W2103090750 @default.
• W2955606500 cites W2107554012 @default.
• W2955606500 cites W2109844604 @default.
• W2955606500 cites W2115955011 @default.
• W2955606500 cites W2121385698 @default.
• W2955606500 cites W2127252546 @default.
• W2955606500 cites W2127845652 @default.
• W2955606500 cites W2138576730 @default.
• W2955606500 cites W2138642050 @default.
• W2955606500 cites W2141859364 @default.
• W2955606500 cites W2154657387 @default.
• W2955606500 cites W2158217645 @default.
• W2955606500 cites W2169222170 @default.
• W2955606500 cites W2186140012 @default.
• W2955606500 cites W2280733329 @default.
• W2955606500 cites W2305217576 @default.
• W2955606500 cites W2314207955 @default.
• W2955606500 cites W2396292748 @default.
• W2955606500 cites W2487844898 @default.
• W2955606500 cites W2537623931 @default.
• W2955606500 cites W2592827120 @default.
• W2955606500 cites W2607729166 @default.
• W2955606500 cites W2746296973 @default.
• W2955606500 cites W2752980047 @default.
• W2955606500 cites W2757339705 @default.
• W2955606500 cites W2758081110 @default.
• W2955606500 cites W2767942359 @default.
• W2955606500 cites W2770032330 @default.
• W2955606500 cites W2773234794 @default.
• W2955606500 cites W2782599677 @default.
• W2955606500 cites W2792995735 @default.
• W2955606500 cites W2793768725 @default.
• W2955606500 cites W2800998762 @default.
• W2955606500 cites W2802729377 @default.
• W2955606500 cites W2887794053 @default.
• W2955606500 doi "https://doi.org/10.1128/msphere.00380-19" @default.
• W2955606500 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6595151" @default.
• W2955606500 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31243080" @default.
• W2955606500 hasPublicationYear "2019" @default.
• W2955606500 type Work @default.
• W2955606500 sameAs 2955606500 @default.
• W2955606500 citedByCount "8" @default.
• W2955606500 countsByYear W29556065002020 @default.
• W2955606500 countsByYear W29556065002021 @default.
• W2955606500 countsByYear W29556065002022 @default.
• W2955606500 countsByYear W29556065002023 @default.
• W2955606500 crossrefType "journal-article" @default.
• W2955606500 hasAuthorship W2955606500A5003790381 @default.
• W2955606500 hasAuthorship W2955606500A5016336211 @default.
• W2955606500 hasAuthorship W2955606500A5018268636 @default.
• W2955606500 hasAuthorship W2955606500A5018962844 @default.
• W2955606500 hasAuthorship W2955606500A5032772458 @default.
• W2955606500 hasAuthorship W2955606500A5033018473 @default.
• W2955606500 hasAuthorship W2955606500A5041639548 @default.
• W2955606500 hasAuthorship W2955606500A5052262478 @default.
• W2955606500 hasAuthorship W2955606500A5069299612 @default.

• next