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• W2955646925 abstract "Arginine is an important amino acid in humankind bodies and is of essential clinical significance. This paper presents a novel bioprobe based on fluorescence resonance energy transfer (FRET), which can be used to detect arginine efficiently and economically. In this bioprobe system, positively charged up-conversion phosphor NaYF4 (NYF) acts as energy donor, and negatively charged gold nanoparticle (AuNP) acts as energy acceptor. The oppositely charged donor and acceptor come into close proximity through electrostatic attraction effect, which results in the occurrence of FRET between NYF and AuNP. The FRET process is thus in the “on” state, meanwhile the system is in the “off” state, and the emitting light of NYF quenched. When positively charged arginine is added into the system, the guanidyl of arginine binds to AuNP and leads to the negatively charged AuNP becomes positively charged one, and the AuNP separates from NYF because of the electrostatic repulsion. The FRET process is blocked and the system switches to the “on” state because the distance between NYF and AuNP becomes longer. In the “on” state, the intensity of the restored emitting light is proportional to the concentration of arginine. This approach brings a good linear relationship between the fluorescence intensity and the concentration of arginine in the concentration range of 14.42–115.04 μM. The limit of detection is as low as 2.9 μM. A new method for quantitative determination of arginine by just measuring the fluorescence intensity of the system is established." @default.
• W2955646925 created "2019-07-12" @default.
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• W2955646925 date "2019-11-01" @default.
• W2955646925 modified "2023-09-27" @default.
• W2955646925 title "A novel arginine bioprobe based on up-conversion fluorescence resonance energy transfer" @default.
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• W2955646925 doi "https://doi.org/10.1016/j.aca.2019.06.060" @default.
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• W2955646925 hasPublicationYear "2019" @default.
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