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- W2955697588 abstract "A simple, sensitive chiral liquid chromatographic method was developed for the separation and quantification of enantiomers Midodrine. A chiral PAK IG-3 (150 x 4.6 mm) 3µm column was used for the separation of the enantiomers. The mobile phase consists of 10 mM ammonium bicarbonate in water and acetonitrile in the ratio of 95:5, v/v with a flow rate of 0.7 ml/min. The detection was done at 290 nm with column temperature maintained at 40°C. The method linear ranged between 10 – 110 μg/ml and 5 – 100 μg/ml for (+) and (-) Midodrine enantiomers. The recovery of the method was found to be in the range of 99.1 to 101.2 %. The detection limit for the (+) and (-) enantiomers was found to be 4 μg/ml and 1 μg/ml, respectively. A simple validated chiral HPLC method with reverse elusion is described for the separation and quantification of the enantiomers of Midodrine in bulk and formulation." @default.
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- W2955697588 date "2019-07-12" @default.
- W2955697588 modified "2023-10-17" @default.
- W2955697588 title "Enantiomeric separation of Midodrine hydrochloride in bulk and pharma-ceutical dosage form by chiral HPLC" @default.
- W2955697588 doi "https://doi.org/10.26452/ijrps.v10i3.1320" @default.
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