Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955758511> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2955758511 endingPage "240" @default.
- W2955758511 startingPage "231" @default.
- W2955758511 abstract "The type of cell death triggered by a particular environmental stimulus influences the outcome of infection or inflammatory disease processes. The ability to identify the cell death pathway that is activated in response to infection is essential for understanding the pathogenesis and host response to infection. Activation of the cysteine protease caspase-1 in various inflammasome complexes indicates that cells are undergoing pyroptosis, a regulated, proinflammatory cell death. Inflammasome assembly and caspase activation can be measured by various methods ranging from detection of inflammasome-dependent cell death, cytokine secretion, cleavage of caspase-1, or the formation of puncta within the cell that contain inflammasome components, such as caspase-1 or the adapter protein ASC. Here we describe a method for detecting caspase-1 activation on a single cell level in the context of infection by the Gram-negative pathogen Yersinia using immunofluorescence microscopy. We previously used this approach to quantify caspase-1 puncta formation in cells containing Yersinia translocon components (Zwack et al., MBio 6:e02095-14, 2015). This is a modification of methods used previously by Broz et al. (Cell Host Microbe 8:471-483, 2010) and Case and Roy (MBio 2:e00117-11, 2011). By taking a microscopy-based approach that allows us to quantify puncta as well as other cell-biological features of infection (i.e., number of bacteria associated with a particular cell; levels of bacterial effector or translocon proteins in caspase-1 puncta-containing cells; or levels or localization of host cellular proteins), we can better quantify the heterogeneity between cells undergoing pyroptosis and cells that are not under the same infection conditions. These approaches have the potential to generate hypotheses that can enable further mechanistic insight into activation of pyroptosis in response to bacterial infection." @default.
- W2955758511 created "2019-07-12" @default.
- W2955758511 creator A5028965776 @default.
- W2955758511 creator A5046556663 @default.
- W2955758511 date "2019-01-01" @default.
- W2955758511 modified "2023-09-25" @default.
- W2955758511 title "Analysis of Inflammasome Activation in Response to Yersinia Infection by Fluorescence Microscopy Detection of Active Caspase-1 Puncta" @default.
- W2955758511 cites W1975593978 @default.
- W2955758511 cites W2008261126 @default.
- W2955758511 cites W2009887653 @default.
- W2955758511 cites W2052853635 @default.
- W2955758511 cites W2061883989 @default.
- W2955758511 cites W2076286334 @default.
- W2955758511 cites W2076319724 @default.
- W2955758511 cites W2080827095 @default.
- W2955758511 cites W2098814976 @default.
- W2955758511 cites W2103195913 @default.
- W2955758511 cites W2118800225 @default.
- W2955758511 cites W2144467901 @default.
- W2955758511 cites W2583964565 @default.
- W2955758511 cites W2744831416 @default.
- W2955758511 doi "https://doi.org/10.1007/978-1-4939-9541-7_16" @default.
- W2955758511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31177442" @default.
- W2955758511 hasPublicationYear "2019" @default.
- W2955758511 type Work @default.
- W2955758511 sameAs 2955758511 @default.
- W2955758511 citedByCount "1" @default.
- W2955758511 countsByYear W29557585112022 @default.
- W2955758511 crossrefType "book-chapter" @default.
- W2955758511 hasAuthorship W2955758511A5028965776 @default.
- W2955758511 hasAuthorship W2955758511A5046556663 @default.
- W2955758511 hasConcept C139964883 @default.
- W2955758511 hasConcept C164027704 @default.
- W2955758511 hasConcept C17172800 @default.
- W2955758511 hasConcept C187831198 @default.
- W2955758511 hasConcept C190283241 @default.
- W2955758511 hasConcept C203014093 @default.
- W2955758511 hasConcept C2776255393 @default.
- W2955758511 hasConcept C2776914184 @default.
- W2955758511 hasConcept C2777209026 @default.
- W2955758511 hasConcept C31573885 @default.
- W2955758511 hasConcept C49039625 @default.
- W2955758511 hasConcept C51785407 @default.
- W2955758511 hasConcept C523546767 @default.
- W2955758511 hasConcept C54355233 @default.
- W2955758511 hasConcept C55493867 @default.
- W2955758511 hasConcept C86803240 @default.
- W2955758511 hasConcept C89423630 @default.
- W2955758511 hasConcept C95444343 @default.
- W2955758511 hasConceptScore W2955758511C139964883 @default.
- W2955758511 hasConceptScore W2955758511C164027704 @default.
- W2955758511 hasConceptScore W2955758511C17172800 @default.
- W2955758511 hasConceptScore W2955758511C187831198 @default.
- W2955758511 hasConceptScore W2955758511C190283241 @default.
- W2955758511 hasConceptScore W2955758511C203014093 @default.
- W2955758511 hasConceptScore W2955758511C2776255393 @default.
- W2955758511 hasConceptScore W2955758511C2776914184 @default.
- W2955758511 hasConceptScore W2955758511C2777209026 @default.
- W2955758511 hasConceptScore W2955758511C31573885 @default.
- W2955758511 hasConceptScore W2955758511C49039625 @default.
- W2955758511 hasConceptScore W2955758511C51785407 @default.
- W2955758511 hasConceptScore W2955758511C523546767 @default.
- W2955758511 hasConceptScore W2955758511C54355233 @default.
- W2955758511 hasConceptScore W2955758511C55493867 @default.
- W2955758511 hasConceptScore W2955758511C86803240 @default.
- W2955758511 hasConceptScore W2955758511C89423630 @default.
- W2955758511 hasConceptScore W2955758511C95444343 @default.
- W2955758511 hasLocation W29557585111 @default.
- W2955758511 hasLocation W29557585112 @default.
- W2955758511 hasOpenAccess W2955758511 @default.
- W2955758511 hasPrimaryLocation W29557585111 @default.
- W2955758511 hasRelatedWork W1891826181 @default.
- W2955758511 hasRelatedWork W1989551786 @default.
- W2955758511 hasRelatedWork W2007506634 @default.
- W2955758511 hasRelatedWork W2028457710 @default.
- W2955758511 hasRelatedWork W2046832808 @default.
- W2955758511 hasRelatedWork W2055408817 @default.
- W2955758511 hasRelatedWork W2060092478 @default.
- W2955758511 hasRelatedWork W2077730689 @default.
- W2955758511 hasRelatedWork W2293295080 @default.
- W2955758511 hasRelatedWork W3211276254 @default.
- W2955758511 isParatext "false" @default.
- W2955758511 isRetracted "false" @default.
- W2955758511 magId "2955758511" @default.
- W2955758511 workType "book-chapter" @default.