Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955860167> ?p ?o ?g. }
- W2955860167 endingPage "1672" @default.
- W2955860167 startingPage "1663" @default.
- W2955860167 abstract "Despite having a great variety of topologies, most DNA, RNA, and RNA-DNA hybrid (RDH) configurations for single-molecule manipulation are composed of several single-stranded (ss) DNA and ssRNA strands, with functional labels at the two ends for surface tethering. On this basis, we developed a simple, robust, and universal amplification-annealing (AA) assay for making all these configurations in two or three steps without inefficient digestion and ligation reactions. As examples, we made ssDNA, short ssDNA with double-stranded (ds) DNA handles, dsDNA with ssDNA handles, replication-fork shaped DNA/RDH/RNA, DNA holiday junction, three-site multiple-labeled and nicked DNA, torsion-constrained RNA/RDH, and short ssRNA with RDH handles. In addition to single-molecule manipulation techniques including optical tweezers, magnetic tweezers, and atomic force microscopy, these configurations can be applied in other surface-tethering techniques as well." @default.
- W2955860167 created "2019-07-12" @default.
- W2955860167 creator A5000891663 @default.
- W2955860167 creator A5026631806 @default.
- W2955860167 creator A5033071749 @default.
- W2955860167 creator A5034016961 @default.
- W2955860167 creator A5034855502 @default.
- W2955860167 creator A5043234782 @default.
- W2955860167 creator A5077810900 @default.
- W2955860167 creator A5080683833 @default.
- W2955860167 creator A5085386508 @default.
- W2955860167 date "2019-07-02" @default.
- W2955860167 modified "2023-10-16" @default.
- W2955860167 title "A Universal Assay for Making DNA, RNA, and RNA–DNA Hybrid Configurations for Single-Molecule Manipulation in Two or Three Steps without Ligation" @default.
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- W2955860167 doi "https://doi.org/10.1021/acssynbio.9b00241" @default.