FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2955874476> ?p ?o ?g. }

• W2955874476 endingPage "124281" @default.
• W2955874476 startingPage "124281" @default.
• W2955874476 abstract "Microcystinase (MlrA) catalyzes the first and most important biodegradation step of hepatotoxic microcystin-LR (MC-LR) produced and released from cyanobacterial cells, and the underlying catalytic mechanism is not completely understood yet. MlrA was postulated previously to be a metalloprotease with an active site of H260AIH263NE265, a variant of the common metal-binding motif of HEXXH. Through comparison with representative modes in HEXXH-containing metalloproteases, molecular dynamics simulation, homology modeling, and docking, the active sites of MlrA involved in the MC-LR biodegradation by Sphingomonas sp. USTB-05 were predicted. Site-directed mutants of MlrA were constructed for verification then. The results show that MlrA is likely not a metalloprotease, but a glutamate protease belonging to type II CAAX prenyl endopeptidases. Combined with the biodegradation of MC-LR by MlrA and its mutants, a complete enzymatic mechanism for MC-LR biodegradation by MlrA is proposed: Glu172 and His205 activate a water molecule facilitating a nucleophilic attack on the Adda-Arg peptide bond of MC-LR; Trp176 and Trp201 contact the carboxylate side chain of Glu172and, by raising its pKa potentially, accelerate the reaction rates; His260 and Asn264 (located in the previous postulated active center of H260AIH263NE265) function as an oxyanion hole to stabilize the transition states. This study reveals the enzymatic mechanism of MlrA for catalyzing MC-LR in both the representative modes and the experiments of site-directed mutagenesis." @default.
• W2955874476 created "2019-07-12" @default.
• W2955874476 creator A5018838747 @default.
• W2955874476 creator A5023363049 @default.
• W2955874476 creator A5023513916 @default.
• W2955874476 creator A5046212412 @default.
• W2955874476 creator A5048032319 @default.
• W2955874476 creator A5056986683 @default.
• W2955874476 creator A5063889804 @default.
• W2955874476 creator A5070085150 @default.
• W2955874476 creator A5091118994 @default.
• W2955874476 date "2019-12-01" @default.
• W2955874476 modified "2023-10-16" @default.
• W2955874476 title "Structural basis of microcystinase activity for biodegrading microcystin-LR" @default.
• W2955874476 cites W1031578623 @default.
• W2955874476 cites W1488354887 @default.
• W2955874476 cites W1803102843 @default.
• W2955874476 cites W1969048724 @default.
• W2955874476 cites W1971059401 @default.
• W2955874476 cites W1973440793 @default.
• W2955874476 cites W1974792663 @default.
• W2955874476 cites W1976161355 @default.
• W2955874476 cites W1976499671 @default.
• W2955874476 cites W1977548768 @default.
• W2955874476 cites W1981264193 @default.
• W2955874476 cites W1986894327 @default.
• W2955874476 cites W1996423252 @default.
• W2955874476 cites W1999191524 @default.
• W2955874476 cites W2001326121 @default.
• W2955874476 cites W2013897148 @default.
• W2955874476 cites W2014725801 @default.
• W2955874476 cites W2017810877 @default.
• W2955874476 cites W2021807514 @default.
• W2955874476 cites W2029667189 @default.
• W2955874476 cites W2030758085 @default.
• W2955874476 cites W2043110757 @default.
• W2955874476 cites W2046412723 @default.
• W2955874476 cites W2051282756 @default.
• W2955874476 cites W2053000830 @default.
• W2955874476 cites W2053358265 @default.
• W2955874476 cites W2066935601 @default.
• W2955874476 cites W2067102012 @default.
• W2955874476 cites W2069714305 @default.
• W2955874476 cites W2073798474 @default.
• W2955874476 cites W2078301833 @default.
• W2955874476 cites W2082621592 @default.
• W2955874476 cites W2098646455 @default.
• W2955874476 cites W2127322768 @default.
• W2955874476 cites W2141941210 @default.
• W2955874476 cites W2146256775 @default.
• W2955874476 cites W2147993766 @default.
• W2955874476 cites W2149525061 @default.
• W2955874476 cites W2151268516 @default.
• W2955874476 cites W2155413971 @default.
• W2955874476 cites W2159527263 @default.
• W2955874476 cites W2161605421 @default.
• W2955874476 cites W2164572388 @default.
• W2955874476 cites W2224056471 @default.
• W2955874476 cites W2297465100 @default.
• W2955874476 cites W2318066609 @default.
• W2955874476 cites W2326408903 @default.
• W2955874476 cites W2504007471 @default.
• W2955874476 cites W2559191318 @default.
• W2955874476 cites W2578123690 @default.
• W2955874476 cites W2605955550 @default.
• W2955874476 cites W2616074949 @default.
• W2955874476 cites W2741668139 @default.
• W2955874476 cites W2790104596 @default.
• W2955874476 cites W2793425294 @default.
• W2955874476 cites W2797232968 @default.
• W2955874476 cites W2802815640 @default.
• W2955874476 cites W2809204969 @default.
• W2955874476 cites W4234797890 @default.
• W2955874476 doi "https://doi.org/10.1016/j.chemosphere.2019.07.012" @default.
• W2955874476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31310980" @default.
• W2955874476 hasPublicationYear "2019" @default.
• W2955874476 type Work @default.
• W2955874476 sameAs 2955874476 @default.
• W2955874476 citedByCount "13" @default.
• W2955874476 countsByYear W29558744762019 @default.
• W2955874476 countsByYear W29558744762020 @default.
• W2955874476 countsByYear W29558744762021 @default.
• W2955874476 countsByYear W29558744762022 @default.
• W2955874476 countsByYear W29558744762023 @default.
• W2955874476 crossrefType "journal-article" @default.
• W2955874476 hasAuthorship W2955874476A5018838747 @default.
• W2955874476 hasAuthorship W2955874476A5023363049 @default.
• W2955874476 hasAuthorship W2955874476A5023513916 @default.
• W2955874476 hasAuthorship W2955874476A5046212412 @default.
• W2955874476 hasAuthorship W2955874476A5048032319 @default.
• W2955874476 hasAuthorship W2955874476A5056986683 @default.
• W2955874476 hasAuthorship W2955874476A5063889804 @default.
• W2955874476 hasAuthorship W2955874476A5070085150 @default.
• W2955874476 hasAuthorship W2955874476A5091118994 @default.
• W2955874476 hasConcept C104317684 @default.
• W2955874476 hasConcept C107824862 @default.
• W2955874476 hasConcept C143065580 @default.
• W2955874476 hasConcept C145770059 @default.

• next