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• W2956064403 endingPage "13618" @default.
• W2956064403 startingPage "13604" @default.
• W2956064403 abstract "[NiFe]-hydrogenase enzymes catalyze the reversible oxidation of hydrogen at a bimetallic cluster and are used by bacteria and archaea for anaerobic growth and pathogenesis. Maturation of the [NiFe]-hydrogenase requires several accessory proteins to assemble and insert the components of the active site. The penultimate maturation step is the delivery of nickel to a primed hydrogenase enzyme precursor protein, a process that is accomplished by two nickel metallochaperones, the accessory protein HypA and the GTPase HypB. Recent work demonstrated that nickel is rapidly transferred to HypA from GDP-loaded HypB within the context of a protein complex in a nickel selective and unidirectional process. To investigate the mechanism of metal transfer, we examined the allosteric effects of nucleotide cofactors and partner proteins on the nickel environments of HypA and HypB by using a combination of biochemical, microbiological, computational, and spectroscopic techniques. We observed that loading HypB with either GDP or a nonhydrolyzable GTP analogue resulted in a similar nickel environment. In addition, interaction with a mutant version of HypA with disrupted nickel binding, H2Q-HypA, does not induce substantial changes to the HypB G-domain nickel site. Instead, the results demonstrate that HypB modifies the acceptor site of HypA. Analysis of a peptide maquette derived from the N-terminus of HypA revealed that nickel is predominately coordinated by atoms from the N-terminal Met-His motif. Furthermore, HypA is capable of two nickel-binding modes at the N-terminus, a HypB-induced mode and a binding mode that mirrors the peptide maquette. Collectively, these results reveal that HypB brings about changes in the nickel coordination of HypA, providing a mechanism for the HypB-dependent control of the acquisition and release of nickel by HypA." @default.
• W2956064403 created "2019-07-12" @default.
• W2956064403 creator A5022764384 @default.
• W2956064403 creator A5033962588 @default.
• W2956064403 creator A5049226429 @default.
• W2956064403 creator A5054664920 @default.
• W2956064403 creator A5058639044 @default.
• W2956064403 date "2019-07-05" @default.
• W2956064403 modified "2023-10-06" @default.
• W2956064403 title "Bimodal Nickel-Binding Site on <i>Escherichia coli</i> [NiFe]-Hydrogenase Metallochaperone HypA" @default.
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• W2956064403 doi "https://doi.org/10.1021/acs.inorgchem.9b00897" @default.
• W2956064403 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6803039" @default.

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