Matches in SemOpenAlex for { <https://semopenalex.org/work/W2956080765> ?p ?o ?g. }
- W2956080765 abstract "Autoimmune diseases result from aberrant immune attacks by the body itself. It is mysterious how autoantigens, a large cohort of seemingly unconnected molecules expressed in different parts of the body, can induce similar autoimmune responses. We have previously found that dermatan sulfate (DS) can form complexes with molecules of apoptotic cells and stimulate autoreactive CD5+ B cells to produce autoantibodies. Hence, autoantigenic molecules share a unique biochemical property in their affinity to DS. This study sought to further test this uniform principle of autoantigenicity.Proteomes were extracted from freshly collected mouse livers. They were loaded onto columns packed with DS-Sepharose resins. Proteins were eluted with step gradients of increasing salt strength. Proteins that bound to DS with weak, moderate, or strong affinity were eluted with 0.4, 0.6, and 1.0 M NaCl, respectively. After desalting, trypsin digestion, and gel electrophoresis, proteins were sequenced by mass spectrometry. To validate whether these proteins have been previously identified as autoantigens, an extensive literature search was conducted using the protein name or its alternative names as keywords. Of the 41 proteins identified from the strong DS-affinity fraction, 33 (80%) were verified autoantigens. Of the 46 proteins with moderate DS-affinity, 27 (59%) were verified autoantigens. Of the 125 proteins with weak DS-affinity, 44 (35%) were known autoantigens. Strikingly, these autoantigens fell into the classical autoantibody categories of autoimmune liver diseases: ANA (anti-nuclear autoantibodies), SMA (anti-smooth muscle autoantibodies), AMA (anti-mitochondrial autoantibodies), and LKM (liver-kidney microsomal autoantigens).This study of DS-affinity enrichment of liver proteins establishes a comprehensive autoantigen-ome for autoimmune liver diseases, yielding 104 verified and 108 potential autoantigens. The liver autoantigen-ome sheds light on the molecular origins of autoimmune liver diseases and further supports the notion of a unifying biochemical principle of autoantigenicity." @default.
- W2956080765 created "2019-07-12" @default.
- W2956080765 creator A5002327806 @default.
- W2956080765 creator A5015620326 @default.
- W2956080765 creator A5024064130 @default.
- W2956080765 creator A5037381154 @default.
- W2956080765 date "2019-06-26" @default.
- W2956080765 modified "2023-10-10" @default.
- W2956080765 title "A comprehensive autoantigen-ome of autoimmune liver diseases identified from dermatan sulfate affinity enrichment of liver tissue proteins" @default.
- W2956080765 cites W1487845055 @default.
- W2956080765 cites W1498846365 @default.
- W2956080765 cites W1519924338 @default.
- W2956080765 cites W1519935522 @default.
- W2956080765 cites W1571029847 @default.
- W2956080765 cites W1596627601 @default.
- W2956080765 cites W1603081582 @default.
- W2956080765 cites W1628068646 @default.
- W2956080765 cites W1860140559 @default.
- W2956080765 cites W190325642 @default.
- W2956080765 cites W192937535 @default.
- W2956080765 cites W1933656698 @default.
- W2956080765 cites W1960002746 @default.
- W2956080765 cites W1965380773 @default.
- W2956080765 cites W1970682352 @default.
- W2956080765 cites W1974440177 @default.
- W2956080765 cites W1975896031 @default.
- W2956080765 cites W1987496501 @default.
- W2956080765 cites W1988333614 @default.
- W2956080765 cites W1988428427 @default.
- W2956080765 cites W1995932025 @default.
- W2956080765 cites W2005739235 @default.
- W2956080765 cites W2010174826 @default.
- W2956080765 cites W2012653022 @default.
- W2956080765 cites W2014954438 @default.
- W2956080765 cites W2022893506 @default.
- W2956080765 cites W2023563040 @default.
- W2956080765 cites W2024572138 @default.
- W2956080765 cites W2027910619 @default.
- W2956080765 cites W2028367671 @default.
- W2956080765 cites W2031954720 @default.
- W2956080765 cites W2033513826 @default.
- W2956080765 cites W2034676316 @default.
- W2956080765 cites W2041143359 @default.
- W2956080765 cites W2043163828 @default.
- W2956080765 cites W2043236094 @default.
- W2956080765 cites W2045199144 @default.
- W2956080765 cites W2046054174 @default.
- W2956080765 cites W2048824929 @default.
- W2956080765 cites W2049429671 @default.
- W2956080765 cites W2051515276 @default.
- W2956080765 cites W2053007092 @default.
- W2956080765 cites W2053613115 @default.
- W2956080765 cites W2054315248 @default.
- W2956080765 cites W2058684713 @default.
- W2956080765 cites W2060354323 @default.
- W2956080765 cites W2062402155 @default.
- W2956080765 cites W2066406096 @default.
- W2956080765 cites W2068139822 @default.
- W2956080765 cites W2069053046 @default.
- W2956080765 cites W2070079669 @default.
- W2956080765 cites W2078880622 @default.
- W2956080765 cites W2082411507 @default.
- W2956080765 cites W2083359560 @default.
- W2956080765 cites W2083365258 @default.
- W2956080765 cites W2085860353 @default.
- W2956080765 cites W2087358521 @default.
- W2956080765 cites W2091533776 @default.
- W2956080765 cites W2094069017 @default.
- W2956080765 cites W2094530006 @default.
- W2956080765 cites W2109199228 @default.
- W2956080765 cites W2109834875 @default.
- W2956080765 cites W2118389028 @default.
- W2956080765 cites W2121960829 @default.
- W2956080765 cites W2123074088 @default.
- W2956080765 cites W2134723896 @default.
- W2956080765 cites W2137901656 @default.
- W2956080765 cites W2138557113 @default.
- W2956080765 cites W2152363933 @default.
- W2956080765 cites W2154919839 @default.
- W2956080765 cites W2154955118 @default.
- W2956080765 cites W2155216250 @default.
- W2956080765 cites W2161529239 @default.
- W2956080765 cites W2162415760 @default.
- W2956080765 cites W2164929581 @default.
- W2956080765 cites W2165441271 @default.
- W2956080765 cites W2168624940 @default.
- W2956080765 cites W2170200755 @default.
- W2956080765 cites W2171593316 @default.
- W2956080765 cites W2208866624 @default.
- W2956080765 cites W2238529478 @default.
- W2956080765 cites W2271112460 @default.
- W2956080765 cites W2280324618 @default.
- W2956080765 cites W2394889634 @default.
- W2956080765 cites W2402501260 @default.
- W2956080765 cites W2408002563 @default.
- W2956080765 cites W2475318574 @default.
- W2956080765 cites W2538855466 @default.
- W2956080765 cites W2548156535 @default.
- W2956080765 cites W2570288598 @default.
- W2956080765 cites W2588091701 @default.